GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
Over the past two decades soybeans grown in western Canada have persistently had lower seed protein than those grown in eastern Canada. To understand the discrepancy in seed protein content between eastern- and western-grown soybeans, RNA-seq and differential expression analysis have been investigat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/18/2337 |
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| author | Julia C. Hooker Nour Nissan Doris Luckert Gerardo Zapata Anfu Hou Ramona M. Mohr Aaron J. Glenn Brent Barlow Ketema A. Daba Thomas D. Warkentin François Lefebvre Ashkan Golshani Elroy R. Cober Bahram Samanfar |
| author_facet | Julia C. Hooker Nour Nissan Doris Luckert Gerardo Zapata Anfu Hou Ramona M. Mohr Aaron J. Glenn Brent Barlow Ketema A. Daba Thomas D. Warkentin François Lefebvre Ashkan Golshani Elroy R. Cober Bahram Samanfar |
| author_sort | Julia C. Hooker |
| collection | DOAJ |
| description | Over the past two decades soybeans grown in western Canada have persistently had lower seed protein than those grown in eastern Canada. To understand the discrepancy in seed protein content between eastern- and western-grown soybeans, RNA-seq and differential expression analysis have been investigated. Ten soybean genotypes, ranging from low to high in seed protein content, were grown in four locations across eastern (Ottawa) and western (Morden, Brandon, and Saskatoon) Canada. Differential expression analysis revealed 34 differentially expressed genes encoding <i>Glycine max</i> Sugars Will Eventually be Exported Transporters (GmSWEETs), including paralogs GmSWEET29 and GmSWEET34 (AtSWEET2 homologs) that were consistently upregulated across all ten genotypes in each of the western locations over three years. GmSWEET29 and GmSWEET34 are likely candidates underlying the lower seed protein content of western soybeans. GmSWEET20 (AtSWEET12 homolog) was downregulated in the western locations and may also play a role in lower seed protein content. These findings are valuable for improving soybean agriculture in western growing regions, establishing more strategic and efficient agricultural practices. |
| first_indexed | 2024-03-09T22:47:29Z |
| format | Article |
| id | doaj.art-7b8f8a5a1d694e1a81479a9d3c22cd93 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T22:47:29Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-7b8f8a5a1d694e1a81479a9d3c22cd932023-11-23T18:26:04ZengMDPI AGPlants2223-77472022-09-011118233710.3390/plants11182337GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western CanadaJulia C. Hooker0Nour Nissan1Doris Luckert2Gerardo Zapata3Anfu Hou4Ramona M. Mohr5Aaron J. Glenn6Brent Barlow7Ketema A. Daba8Thomas D. Warkentin9François Lefebvre10Ashkan Golshani11Elroy R. Cober12Bahram Samanfar13Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaAgriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaAgriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaCanadian Centre for Computational Genomics, Montréal, QC H3A 0G1, CanadaAgriculture and Agri-Food Canada, Morden, MB R6M 1Y5, CanadaAgriculture and Agri-Food Canada, Brandon, MB R7A 5Y3, CanadaAgriculture and Agri-Food Canada, Brandon, MB R7A 5Y3, CanadaCrop Development Centre, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaCrop Development Centre, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaCrop Development Centre, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaCanadian Centre for Computational Genomics, Montréal, QC H3A 0G1, CanadaDepartment of Biology, Ottawa Institute of Systems Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaAgriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaAgriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaOver the past two decades soybeans grown in western Canada have persistently had lower seed protein than those grown in eastern Canada. To understand the discrepancy in seed protein content between eastern- and western-grown soybeans, RNA-seq and differential expression analysis have been investigated. Ten soybean genotypes, ranging from low to high in seed protein content, were grown in four locations across eastern (Ottawa) and western (Morden, Brandon, and Saskatoon) Canada. Differential expression analysis revealed 34 differentially expressed genes encoding <i>Glycine max</i> Sugars Will Eventually be Exported Transporters (GmSWEETs), including paralogs GmSWEET29 and GmSWEET34 (AtSWEET2 homologs) that were consistently upregulated across all ten genotypes in each of the western locations over three years. GmSWEET29 and GmSWEET34 are likely candidates underlying the lower seed protein content of western soybeans. GmSWEET20 (AtSWEET12 homolog) was downregulated in the western locations and may also play a role in lower seed protein content. These findings are valuable for improving soybean agriculture in western growing regions, establishing more strategic and efficient agricultural practices.https://www.mdpi.com/2223-7747/11/18/2337<i>Glycine max</i>SWEETsugar transportRNA-seqtranscriptomics |
| spellingShingle | Julia C. Hooker Nour Nissan Doris Luckert Gerardo Zapata Anfu Hou Ramona M. Mohr Aaron J. Glenn Brent Barlow Ketema A. Daba Thomas D. Warkentin François Lefebvre Ashkan Golshani Elroy R. Cober Bahram Samanfar GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada Plants <i>Glycine max</i> SWEET sugar transport RNA-seq transcriptomics |
| title | GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada |
| title_full | GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada |
| title_fullStr | GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada |
| title_full_unstemmed | GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada |
| title_short | GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada |
| title_sort | gmsweet29 and paralog gmsweet34 are differentially expressed between soybeans grown in eastern and western canada |
| topic | <i>Glycine max</i> SWEET sugar transport RNA-seq transcriptomics |
| url | https://www.mdpi.com/2223-7747/11/18/2337 |
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