Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry
This study has been designed to analyse the secreted and soluble proteins in bovine seminal plasma by mass spectrometry. The majority of these proteins are produced by accessory glands, and partially by testis, epididymis, ductus deferens and vas deferens of male reproductive tract. Seminal plasma...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2019-12-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/3308 |
| _version_ | 1797364089399279616 |
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| author | Hazem Almhanna |
| author_facet | Hazem Almhanna |
| author_sort | Hazem Almhanna |
| collection | DOAJ |
| description | This study has been designed to analyse the secreted and soluble proteins in bovine seminal plasma by mass spectrometry. The majority of these proteins are produced by accessory glands, and partially by testis, epididymis, ductus deferens and vas deferens of male reproductive tract.
Seminal plasma of bovine was collected freshly and isolated after centrifuged and removed the sperm. Non boiled and boiled seminal plasma lysate were run to identify and detect the total proteins, as well as the individual single member of ADAMs protein is determined.
The non-boiled lysate of seminal plasma was displayed diversity of proteins more than boiled sample lysate of seminal plasma.
However, boiled and non-boiled seminal plasma have distinguished several types of ADAMs protein which are included: ADAM10, ADAM9, ADAM7, ADAM15 in non-boiled samples, while boiled lysate of bovine seminal plasma was displayed ADAM10, ADAM9, ADAM28, and ADAM22.
Our finding concluded that bovine seminal plasma is very rich in different types of soluble, cleaved and shed proteins such ADAMs protein which could potentially have a biological and physiological role in protection and interactions of sperm during motility inside female reproductive tract, as it might support it to fertilise the ovum. This protection might be via immunosuppression behaviour or by block specific receptors in female reproductive tract for enhancing sperm motility, and avoid sperm the singling response of immunity. |
| first_indexed | 2024-03-08T16:30:20Z |
| format | Article |
| id | doaj.art-7b8f9f6a481f43bd9f4aeceeb56c712e |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:30:20Z |
| publishDate | 2019-12-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-7b8f9f6a481f43bd9f4aeceeb56c712e2024-01-06T10:11:45ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782019-12-0110210.36326/kjvs/2019/v10i23308Characterisation of ADAMs (protein)of bovine seminal plasma by Mass SpectrometryHazem Almhanna0Department of anatomy, histology and embryology, faculty of veterinary medicine, University of KufaThis study has been designed to analyse the secreted and soluble proteins in bovine seminal plasma by mass spectrometry. The majority of these proteins are produced by accessory glands, and partially by testis, epididymis, ductus deferens and vas deferens of male reproductive tract. Seminal plasma of bovine was collected freshly and isolated after centrifuged and removed the sperm. Non boiled and boiled seminal plasma lysate were run to identify and detect the total proteins, as well as the individual single member of ADAMs protein is determined. The non-boiled lysate of seminal plasma was displayed diversity of proteins more than boiled sample lysate of seminal plasma. However, boiled and non-boiled seminal plasma have distinguished several types of ADAMs protein which are included: ADAM10, ADAM9, ADAM7, ADAM15 in non-boiled samples, while boiled lysate of bovine seminal plasma was displayed ADAM10, ADAM9, ADAM28, and ADAM22. Our finding concluded that bovine seminal plasma is very rich in different types of soluble, cleaved and shed proteins such ADAMs protein which could potentially have a biological and physiological role in protection and interactions of sperm during motility inside female reproductive tract, as it might support it to fertilise the ovum. This protection might be via immunosuppression behaviour or by block specific receptors in female reproductive tract for enhancing sperm motility, and avoid sperm the singling response of immunity.https://journal.uokufa.edu.iq/index.php/kjvs/article/view/3308 ADAMsBovine seminal plasmaImmunosuppression and MotilityMass SpectrometryProteins |
| spellingShingle | Hazem Almhanna Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry Kufa Journal for Veterinary Medical Sciences ADAMs Bovine seminal plasma Immunosuppression and Motility Mass Spectrometry Proteins |
| title | Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry |
| title_full | Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry |
| title_fullStr | Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry |
| title_full_unstemmed | Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry |
| title_short | Characterisation of ADAMs (protein)of bovine seminal plasma by Mass Spectrometry |
| title_sort | characterisation of adams protein of bovine seminal plasma by mass spectrometry |
| topic | ADAMs Bovine seminal plasma Immunosuppression and Motility Mass Spectrometry Proteins |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/3308 |
| work_keys_str_mv | AT hazemalmhanna characterisationofadamsproteinofbovineseminalplasmabymassspectrometry |