A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot study
The present work defines a modified critical size rabbit ulna defect model for bone regeneration in which a non-resorbable barrier membrane was used to separate the radius from the ulna to create a valid model for evaluation of tissue-engineered periosteal substitutes. Eight rabbits divided into two...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fbioe.2014.00080/full |
| _version_ | 1818213835136303104 |
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| author | Rania M El Backly Rania M El Backly Rania M El Backly Danilo eChiapale Anita eMuraglia Giuliana eTromba Chiara eOttonello Federico eSantolini Ranieri eCancedda Ranieri eCancedda Maddalena eMastrogiacomo Maddalena eMastrogiacomo |
| author_facet | Rania M El Backly Rania M El Backly Rania M El Backly Danilo eChiapale Anita eMuraglia Giuliana eTromba Chiara eOttonello Federico eSantolini Ranieri eCancedda Ranieri eCancedda Maddalena eMastrogiacomo Maddalena eMastrogiacomo |
| author_sort | Rania M El Backly |
| collection | DOAJ |
| description | The present work defines a modified critical size rabbit ulna defect model for bone regeneration in which a non-resorbable barrier membrane was used to separate the radius from the ulna to create a valid model for evaluation of tissue-engineered periosteal substitutes. Eight rabbits divided into two groups were used. Critical defects (15 mm) were made in the ulna completely eliminating periosteum. For group I, defects were filled with a nanohydroxyapatite poly(ester urethane) scaffold soaked in PBS and left as such (group Ia) or wrapped with a tissue-engineered periosteal substitute (group Ib). For group II, an e-PTFE (GORE-TEX®) membrane was inserted around the radius then the defects received either scaffold alone (group IIa) or scaffold wrapped with periosteal substitute (group IIb). Animals were euthanized after 12-16 weeks, and bone regeneration was evaluated by radiography, computed microtomography (µCT), and histology. In the first group, we observed formation of radio-ulnar synostosis irrespective of the treatment. This was completely eliminated upon placement of the e-PTFE (GORE-TEX®) membrane in the second group of animals. In conclusion, modification of the model using a non-resorbable e-PTFE membrane to isolate the ulna from the radius was a valuable addition allowing for objective evaluation of the tissue-engineered periosteal substitute. |
| first_indexed | 2024-12-12T06:10:36Z |
| format | Article |
| id | doaj.art-7b9028226dc246eab85e9809c85f85b1 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T06:10:36Z |
| publishDate | 2015-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-7b9028226dc246eab85e9809c85f85b12022-12-22T00:35:10ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852015-01-01210.3389/fbioe.2014.00080124948A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot studyRania M El Backly0Rania M El Backly1Rania M El Backly2Danilo eChiapale3Anita eMuraglia4Giuliana eTromba5Chiara eOttonello6Federico eSantolini7Ranieri eCancedda8Ranieri eCancedda9Maddalena eMastrogiacomo10Maddalena eMastrogiacomo11University of GenoaIRCCS AOU San Martino–IST Istituto Nazionale per la Ricerca sul CancroAlexandria UniversityIRCCS AOU San Martino–IST Istituto Nazionale per la Ricerca sul CancroBiorigen SrlSincrotrone Trieste S.C.p.A.Biorigen SrlIRCCS AOU San Martino–IST Istituto Nazionale per la Ricerca sul CancroUniversity of GenoaIRCCS AOU San Martino–IST Istituto Nazionale per la Ricerca sul CancroUniversity of GenoaIRCCS AOU San Martino–IST Istituto Nazionale per la Ricerca sul CancroThe present work defines a modified critical size rabbit ulna defect model for bone regeneration in which a non-resorbable barrier membrane was used to separate the radius from the ulna to create a valid model for evaluation of tissue-engineered periosteal substitutes. Eight rabbits divided into two groups were used. Critical defects (15 mm) were made in the ulna completely eliminating periosteum. For group I, defects were filled with a nanohydroxyapatite poly(ester urethane) scaffold soaked in PBS and left as such (group Ia) or wrapped with a tissue-engineered periosteal substitute (group Ib). For group II, an e-PTFE (GORE-TEX®) membrane was inserted around the radius then the defects received either scaffold alone (group IIa) or scaffold wrapped with periosteal substitute (group IIb). Animals were euthanized after 12-16 weeks, and bone regeneration was evaluated by radiography, computed microtomography (µCT), and histology. In the first group, we observed formation of radio-ulnar synostosis irrespective of the treatment. This was completely eliminated upon placement of the e-PTFE (GORE-TEX®) membrane in the second group of animals. In conclusion, modification of the model using a non-resorbable e-PTFE membrane to isolate the ulna from the radius was a valuable addition allowing for objective evaluation of the tissue-engineered periosteal substitute.http://journal.frontiersin.org/Journal/10.3389/fbioe.2014.00080/fullBone RegenerationPlatelet-Rich PlasmaCritical size defecte-PTFEperiosteal substitute |
| spellingShingle | Rania M El Backly Rania M El Backly Rania M El Backly Danilo eChiapale Anita eMuraglia Giuliana eTromba Chiara eOttonello Federico eSantolini Ranieri eCancedda Ranieri eCancedda Maddalena eMastrogiacomo Maddalena eMastrogiacomo A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot study Frontiers in Bioengineering and Biotechnology Bone Regeneration Platelet-Rich Plasma Critical size defect e-PTFE periosteal substitute |
| title | A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot study |
| title_full | A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot study |
| title_fullStr | A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot study |
| title_full_unstemmed | A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot study |
| title_short | A modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering: a pilot study |
| title_sort | modified rabbit ulna defect model for evaluating periosteal substitutes in bone engineering a pilot study |
| topic | Bone Regeneration Platelet-Rich Plasma Critical size defect e-PTFE periosteal substitute |
| url | http://journal.frontiersin.org/Journal/10.3389/fbioe.2014.00080/full |
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