Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study
Although single-lipid bilayers are usually considered models of eukaryotic plasma membranes, their research drops drastically when it comes to exclusively anionic lipid membranes. Being a major anionic phospholipid in the inner leaflet of eukaryote membranes, phosphatidylserine-constituted lipid mem...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Membranes |
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| Online Access: | https://www.mdpi.com/2077-0375/13/1/83 |
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| author | Lea Pašalić Barbara Pem Danijela Bakarić |
| author_facet | Lea Pašalić Barbara Pem Danijela Bakarić |
| author_sort | Lea Pašalić |
| collection | DOAJ |
| description | Although single-lipid bilayers are usually considered models of eukaryotic plasma membranes, their research drops drastically when it comes to exclusively anionic lipid membranes. Being a major anionic phospholipid in the inner leaflet of eukaryote membranes, phosphatidylserine-constituted lipid membranes were occasionally explored in the form of multilamellar liposomes (MLV), but their inherent instability caused a serious lack of efforts undertaken on large unilamellar liposomes (LUVs) as more realistic model membrane systems. In order to compensate the existing shortcomings, we performed a comprehensive calorimetric, spectroscopic and MD simulation study of time-varying structural features of LUV made from 1,2-dipalmitoyl-<i>sn</i>-glycero-3-phospho-L-serine (DPPS), whereas the corresponding MLV were examined as a reference. A substantial uncertainty of UV/Vis data of LUV from which only <i>T</i><sub>m</sub> was unambiguously determined (53.9 ± 0.8 °C), along with rather high uncertainty on the high-temperature range of DPPS melting profile obtained from DSC (≈50–59 °C), presumably reflect distinguished surface structural features in LUV. The FTIR signatures of glycerol moiety and those originated from carboxyl group serve as a strong support that in LUV, unlike in MLV, highly curved surfaces occur continuously, whereas the details on the attenuation of surface features in MLV were unraveled by molecular dynamics. |
| first_indexed | 2024-03-09T11:43:36Z |
| format | Article |
| id | doaj.art-7b90f2a4184e4343af5af0fb6cded4dd |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-09T11:43:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-7b90f2a4184e4343af5af0fb6cded4dd2023-11-30T23:26:59ZengMDPI AGMembranes2077-03752023-01-011318310.3390/membranes13010083Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational StudyLea Pašalić0Barbara Pem1Danijela Bakarić2Division for Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, CroatiaDivision for Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, CroatiaDivision for Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, CroatiaAlthough single-lipid bilayers are usually considered models of eukaryotic plasma membranes, their research drops drastically when it comes to exclusively anionic lipid membranes. Being a major anionic phospholipid in the inner leaflet of eukaryote membranes, phosphatidylserine-constituted lipid membranes were occasionally explored in the form of multilamellar liposomes (MLV), but their inherent instability caused a serious lack of efforts undertaken on large unilamellar liposomes (LUVs) as more realistic model membrane systems. In order to compensate the existing shortcomings, we performed a comprehensive calorimetric, spectroscopic and MD simulation study of time-varying structural features of LUV made from 1,2-dipalmitoyl-<i>sn</i>-glycero-3-phospho-L-serine (DPPS), whereas the corresponding MLV were examined as a reference. A substantial uncertainty of UV/Vis data of LUV from which only <i>T</i><sub>m</sub> was unambiguously determined (53.9 ± 0.8 °C), along with rather high uncertainty on the high-temperature range of DPPS melting profile obtained from DSC (≈50–59 °C), presumably reflect distinguished surface structural features in LUV. The FTIR signatures of glycerol moiety and those originated from carboxyl group serve as a strong support that in LUV, unlike in MLV, highly curved surfaces occur continuously, whereas the details on the attenuation of surface features in MLV were unraveled by molecular dynamics.https://www.mdpi.com/2077-0375/13/1/831,2-dipalmitoyl-<i>sn</i>-glycero-3-phospho-L-serine sodium salt (DPPS)multilamellar and large unilamellar vesicles (MLV and LUV)surface curvature fluctuationsinterbilayer waterspectroscopic and calorimetric studyMD simulations |
| spellingShingle | Lea Pašalić Barbara Pem Danijela Bakarić Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study Membranes 1,2-dipalmitoyl-<i>sn</i>-glycero-3-phospho-L-serine sodium salt (DPPS) multilamellar and large unilamellar vesicles (MLV and LUV) surface curvature fluctuations interbilayer water spectroscopic and calorimetric study MD simulations |
| title | Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study |
| title_full | Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study |
| title_fullStr | Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study |
| title_full_unstemmed | Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study |
| title_short | Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study |
| title_sort | lamellarity driven differences in surface structural features of dpps lipids spectroscopic calorimetric and computational study |
| topic | 1,2-dipalmitoyl-<i>sn</i>-glycero-3-phospho-L-serine sodium salt (DPPS) multilamellar and large unilamellar vesicles (MLV and LUV) surface curvature fluctuations interbilayer water spectroscopic and calorimetric study MD simulations |
| url | https://www.mdpi.com/2077-0375/13/1/83 |
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