MicroRNA-Related Polymorphism and Their Association with Fibromyalgia
MicroRNAs are tissue-specific expressed short RNAs that serve post-transcriptional gene regulation. A specific microRNA can bind to mRNAs of different genes and thereby suppress their protein production. In the context of the complex phenotype of fibromyalgia, we used the Axiom miRNA Target Site Gen...
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MDPI AG
2023-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/7/1312 |
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| author | Fabian Berg Dirk A. Moser Verena Hagena Fabian Streit Benjamin Mosch Robert Kumsta Stephan Herpertz Martin Diers |
| author_facet | Fabian Berg Dirk A. Moser Verena Hagena Fabian Streit Benjamin Mosch Robert Kumsta Stephan Herpertz Martin Diers |
| author_sort | Fabian Berg |
| collection | DOAJ |
| description | MicroRNAs are tissue-specific expressed short RNAs that serve post-transcriptional gene regulation. A specific microRNA can bind to mRNAs of different genes and thereby suppress their protein production. In the context of the complex phenotype of fibromyalgia, we used the Axiom miRNA Target Site Genotyping Array to search genome-wide for DNA variations in microRNA genes, their regulatory regions, and in the 3’UTR of protein-coding genes. To identify disease-relevant DNA polymorphisms, a cohort of 176 female fibromyalgia patients was studied in comparison to a cohort of 162 healthy women. The association between 48,329 markers and fibromyalgia was investigated using logistic regression adjusted for population stratification. Results show that 29 markers had <i>p</i>-values < 1 × 10<sup>−3</sup>, and the strongest association was observed for rs758459 (<i>p</i>-value of 0.0001), located in the <i>Neurogenin 1</i> gene which is targeted by hsa-miR-130a-3p. Furthermore, variant rs2295963 is predicted to affect binding of hsa-miR-1-3p. Both microRNAs were previously reported to be differentially expressed in fibromyalgia patients. Despite its limited statistical power, this study reports two microRNA-related polymorphisms which may play a functional role in the pathogenesis of fibromyalgia. For a better understanding of the disease pattern, further functional analyses on the biological significance of microRNAs and microRNA-related polymorphisms are required. |
| first_indexed | 2024-03-11T01:02:44Z |
| format | Article |
| id | doaj.art-7b92248b59364ea79b962f9f74ac3baf |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T01:02:44Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| series | Genes |
| spelling | doaj.art-7b92248b59364ea79b962f9f74ac3baf2023-11-18T19:28:34ZengMDPI AGGenes2073-44252023-06-01147131210.3390/genes14071312MicroRNA-Related Polymorphism and Their Association with FibromyalgiaFabian Berg0Dirk A. Moser1Verena Hagena2Fabian Streit3Benjamin Mosch4Robert Kumsta5Stephan Herpertz6Martin Diers7Department of Genetic Psychology, Faculty of Psychology, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, GermanyDepartment of Genetic Psychology, Faculty of Psychology, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, GermanyDepartment of Psychosomatic Medicine and Psychotherapy, LWL University Hospital, Ruhr University Bochum, 448791 Bochum, GermanyDepartment of Genetic Epidemiology in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, 68159 Mannheim, GermanyDepartment of Psychosomatic Medicine and Psychotherapy, LWL University Hospital, Ruhr University Bochum, 448791 Bochum, GermanyDepartment of Behavioural and Cognitive Sciences, Laboratory for Stress and Gene-Environment Interplay, University of Luxemburg, Porte des Sciences, L-4366 Esch-sur-Alzette, LuxembourgDepartment of Psychosomatic Medicine and Psychotherapy, LWL University Hospital, Ruhr University Bochum, 448791 Bochum, GermanyDepartment of Psychosomatic Medicine and Psychotherapy, LWL University Hospital, Ruhr University Bochum, 448791 Bochum, GermanyMicroRNAs are tissue-specific expressed short RNAs that serve post-transcriptional gene regulation. A specific microRNA can bind to mRNAs of different genes and thereby suppress their protein production. In the context of the complex phenotype of fibromyalgia, we used the Axiom miRNA Target Site Genotyping Array to search genome-wide for DNA variations in microRNA genes, their regulatory regions, and in the 3’UTR of protein-coding genes. To identify disease-relevant DNA polymorphisms, a cohort of 176 female fibromyalgia patients was studied in comparison to a cohort of 162 healthy women. The association between 48,329 markers and fibromyalgia was investigated using logistic regression adjusted for population stratification. Results show that 29 markers had <i>p</i>-values < 1 × 10<sup>−3</sup>, and the strongest association was observed for rs758459 (<i>p</i>-value of 0.0001), located in the <i>Neurogenin 1</i> gene which is targeted by hsa-miR-130a-3p. Furthermore, variant rs2295963 is predicted to affect binding of hsa-miR-1-3p. Both microRNAs were previously reported to be differentially expressed in fibromyalgia patients. Despite its limited statistical power, this study reports two microRNA-related polymorphisms which may play a functional role in the pathogenesis of fibromyalgia. For a better understanding of the disease pattern, further functional analyses on the biological significance of microRNAs and microRNA-related polymorphisms are required.https://www.mdpi.com/2073-4425/14/7/1312fibromyalgia (FM)miRNApleiotropygene regulationepigenetics |
| spellingShingle | Fabian Berg Dirk A. Moser Verena Hagena Fabian Streit Benjamin Mosch Robert Kumsta Stephan Herpertz Martin Diers MicroRNA-Related Polymorphism and Their Association with Fibromyalgia Genes fibromyalgia (FM) miRNA pleiotropy gene regulation epigenetics |
| title | MicroRNA-Related Polymorphism and Their Association with Fibromyalgia |
| title_full | MicroRNA-Related Polymorphism and Their Association with Fibromyalgia |
| title_fullStr | MicroRNA-Related Polymorphism and Their Association with Fibromyalgia |
| title_full_unstemmed | MicroRNA-Related Polymorphism and Their Association with Fibromyalgia |
| title_short | MicroRNA-Related Polymorphism and Their Association with Fibromyalgia |
| title_sort | microrna related polymorphism and their association with fibromyalgia |
| topic | fibromyalgia (FM) miRNA pleiotropy gene regulation epigenetics |
| url | https://www.mdpi.com/2073-4425/14/7/1312 |
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