The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats
Background: Hypothalamus is mentioned as the major center of appetite and energy balance. Physical activity and the exersice are able to disturb the energy balance to negative. Nesfatin-1 is a regulating neuropeptide that is produced by hypothalamus and has an important role in establishing energy b...
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2012-09-01
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| Series: | Iranian South Medical Journal |
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| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-266&slc_lang=en&sid=1 |
| _version_ | 1818139331304357888 |
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| author | Abbas Ghanbari Niaki Fatemeh Hosseinpour Rozita Fathi Alireza Safai-Kenari |
| author_facet | Abbas Ghanbari Niaki Fatemeh Hosseinpour Rozita Fathi Alireza Safai-Kenari |
| author_sort | Abbas Ghanbari Niaki |
| collection | DOAJ |
| description | Background: Hypothalamus is mentioned as the major center of appetite and energy balance. Physical activity and the exersice are able to disturb the energy balance to negative. Nesfatin-1 is a regulating neuropeptide that is produced by hypothalamus and has an important role in establishing energy balance. The purpose of this study was to examine the effect of endurance training regimen on nesfatin-1 gene expression and its concentration in the male rat hypothalamus. Materials and Methods: Eleven adult wistar male rats (8-10 week old, 130-145g) assigned into control(C, n=5) and training (E, n=6) groups. Training group was given exercise on a motor-driven treadmill (20m/min, 0% grade, 60 min/session, 5days/week for 8 weeks). Rats were sacrificed 72h after the last training session and then the hypothalamus tissue was excised for determination of nesfatin-1 gene expression and its concentration by RT-PCR & ELIZA methods, respectively. Four hours before the experiment the food not tap water was removed from the animal cages. Data was analyzed by using an independent t-student test. Results: The current results indicated that the levels of nesfatin-1 gene expression and its concentration, ATP, and glycogen concentrations were non-significantly lower in trained group when compared with control group. Conclusion: This research showed for the first time, that a low-intensity exercises, decreases nesfatin-1 expression and concentration in the hypothalamus, which accompanied insignificant reduction in energy source. It seems that in the present research, the exercise has had the same fasting and being hungry like effect on nesfatin-1 expression and concentration in the hypothalamus. |
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| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-12-11T10:26:23Z |
| publishDate | 2012-09-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-7b9347e991164ca5a524d9e580c2e93a2022-12-22T01:11:09ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542012-09-01153171182The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male ratsAbbas Ghanbari Niaki0Fatemeh Hosseinpour1Rozita Fathi2Alireza Safai-Kenari3 Faculty of Physical Education & Sport Sciences, University of Mazandaran, Mazandaran, IRAN Faculty of Physical Education & Sport Sciences, University of Mazandaran, Mazandaran, IRAN Faculty of Physical Education & Sport Sciences, University of Mazandaran, Mazandaran, IRAN Faculty of Physical Education & Sport Sciences, University of Mazandaran, Mazandaran, IRAN Background: Hypothalamus is mentioned as the major center of appetite and energy balance. Physical activity and the exersice are able to disturb the energy balance to negative. Nesfatin-1 is a regulating neuropeptide that is produced by hypothalamus and has an important role in establishing energy balance. The purpose of this study was to examine the effect of endurance training regimen on nesfatin-1 gene expression and its concentration in the male rat hypothalamus. Materials and Methods: Eleven adult wistar male rats (8-10 week old, 130-145g) assigned into control(C, n=5) and training (E, n=6) groups. Training group was given exercise on a motor-driven treadmill (20m/min, 0% grade, 60 min/session, 5days/week for 8 weeks). Rats were sacrificed 72h after the last training session and then the hypothalamus tissue was excised for determination of nesfatin-1 gene expression and its concentration by RT-PCR & ELIZA methods, respectively. Four hours before the experiment the food not tap water was removed from the animal cages. Data was analyzed by using an independent t-student test. Results: The current results indicated that the levels of nesfatin-1 gene expression and its concentration, ATP, and glycogen concentrations were non-significantly lower in trained group when compared with control group. Conclusion: This research showed for the first time, that a low-intensity exercises, decreases nesfatin-1 expression and concentration in the hypothalamus, which accompanied insignificant reduction in energy source. It seems that in the present research, the exercise has had the same fasting and being hungry like effect on nesfatin-1 expression and concentration in the hypothalamus.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-266&slc_lang=en&sid=1Nesfatin-1 Nucleobindin-2 anorexigenic protein gene expression male rat |
| spellingShingle | Abbas Ghanbari Niaki Fatemeh Hosseinpour Rozita Fathi Alireza Safai-Kenari The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats Iranian South Medical Journal Nesfatin-1 Nucleobindin-2 anorexigenic protein gene expression male rat |
| title | The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats |
| title_full | The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats |
| title_fullStr | The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats |
| title_full_unstemmed | The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats |
| title_short | The effect of 8 weeks of endurance training on hypothalamic Nesfatin-1 gene expression and its concentration in male rats |
| title_sort | effect of 8 weeks of endurance training on hypothalamic nesfatin 1 gene expression and its concentration in male rats |
| topic | Nesfatin-1 Nucleobindin-2 anorexigenic protein gene expression male rat |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-266&slc_lang=en&sid=1 |
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