Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products
Michele E Barbour,1 Sarah E Maddocks,2 Natalie J Wood,1,3 Andrew M Collins3 1Oral Nanoscience, School of Oral and Dental Sciences, University of Bristol, Bristol, UK; 2Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK; 3Bristol Centre for Functional Nanomaterials, Unive...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2013-09-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/synthesis-characterization-and-efficacy-of-antimicrobial-chlorhexidine-a14418 |
| _version_ | 1819289560366972928 |
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| author | Barbour ME Maddocks SE Wood NJ Collins AM |
| author_facet | Barbour ME Maddocks SE Wood NJ Collins AM |
| author_sort | Barbour ME |
| collection | DOAJ |
| description | Michele E Barbour,1 Sarah E Maddocks,2 Natalie J Wood,1,3 Andrew M Collins3 1Oral Nanoscience, School of Oral and Dental Sciences, University of Bristol, Bristol, UK; 2Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK; 3Bristol Centre for Functional Nanomaterials, University of Bristol, Bristol, UK Abstract: Chlorhexidine (CHX) is an antimicrobial agent that is efficacious against gram-negative and -positive bacteria and yeasts. Its mechanism of action is based on cell membrane disruption and, as such, it does not promote the development of bacterial resistance, which is associated with the widespread use of antibiotics. In this manuscript, we report the development of novel antimicrobial nanoparticles (NPs) based on a hexametaphosphate salt of CHX. These are synthesized by instantaneous reaction between equimolar aqueous solutions of CHX digluconate and sodium hexametaphosphate, under room temperature and pressure. The reaction results in a stable colloid composed of highly negatively charged NPs (−50 mV), of size 20-160 nm. The NPs adhere rapidly to specimens of glass, titanium, and an elastomeric wound dressing, in a dose-dependent manner. The functionalized materials exhibit a gradual leaching of soluble CHX over a period of at least 50 days. The NP colloid is efficacious against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in both planktonic and biofilm conditions. These NPs may find application in a range of biomedical and consumer materials. Keywords: MRSA, biomaterials, chlorhexidine, drug delivery, slow release |
| first_indexed | 2024-12-24T03:08:47Z |
| format | Article |
| id | doaj.art-7b945d6ca68341eea8320c1eefec7531 |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-24T03:08:47Z |
| publishDate | 2013-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-7b945d6ca68341eea8320c1eefec75312022-12-21T17:17:54ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132013-09-012013default35073519Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer productsBarbour MEMaddocks SEWood NJCollins AMMichele E Barbour,1 Sarah E Maddocks,2 Natalie J Wood,1,3 Andrew M Collins3 1Oral Nanoscience, School of Oral and Dental Sciences, University of Bristol, Bristol, UK; 2Cardiff School of Health Sciences, Cardiff Metropolitan University, Cardiff, UK; 3Bristol Centre for Functional Nanomaterials, University of Bristol, Bristol, UK Abstract: Chlorhexidine (CHX) is an antimicrobial agent that is efficacious against gram-negative and -positive bacteria and yeasts. Its mechanism of action is based on cell membrane disruption and, as such, it does not promote the development of bacterial resistance, which is associated with the widespread use of antibiotics. In this manuscript, we report the development of novel antimicrobial nanoparticles (NPs) based on a hexametaphosphate salt of CHX. These are synthesized by instantaneous reaction between equimolar aqueous solutions of CHX digluconate and sodium hexametaphosphate, under room temperature and pressure. The reaction results in a stable colloid composed of highly negatively charged NPs (−50 mV), of size 20-160 nm. The NPs adhere rapidly to specimens of glass, titanium, and an elastomeric wound dressing, in a dose-dependent manner. The functionalized materials exhibit a gradual leaching of soluble CHX over a period of at least 50 days. The NP colloid is efficacious against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in both planktonic and biofilm conditions. These NPs may find application in a range of biomedical and consumer materials. Keywords: MRSA, biomaterials, chlorhexidine, drug delivery, slow releasehttp://www.dovepress.com/synthesis-characterization-and-efficacy-of-antimicrobial-chlorhexidine-a14418 |
| spellingShingle | Barbour ME Maddocks SE Wood NJ Collins AM Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products International Journal of Nanomedicine |
| title | Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products |
| title_full | Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products |
| title_fullStr | Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products |
| title_full_unstemmed | Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products |
| title_short | Synthesis, characterization, and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products |
| title_sort | synthesis characterization and efficacy of antimicrobial chlorhexidine hexametaphosphate nanoparticles for applications in biomedical materials and consumer products |
| url | http://www.dovepress.com/synthesis-characterization-and-efficacy-of-antimicrobial-chlorhexidine-a14418 |
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