A conserved function for pericentromeric satellite DNA
A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, whi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/34122 |
| _version_ | 1811200763524808704 |
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| author | Madhav Jagannathan Ryan Cummings Yukiko M Yamashita |
| author_facet | Madhav Jagannathan Ryan Cummings Yukiko M Yamashita |
| author_sort | Madhav Jagannathan |
| collection | DOAJ |
| description | A universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarded as junk, is a critical constituent of the chromosome, allowing the packaging of all chromosomes into a single nucleus. We show that the multi-AT-hook satellite DNA-binding proteins, Drosophila melanogaster D1 and mouse HMGA1, play an evolutionarily conserved role in bundling pericentromeric satellite DNA from heterologous chromosomes into ‘chromocenters’, a cytological association of pericentromeric heterochromatin. Defective chromocenter formation leads to micronuclei formation due to budding from the interphase nucleus, DNA damage and cell death. We propose that chromocenter and satellite DNA serve a fundamental role in encapsulating the full complement of the genome within a single nucleus, the universal characteristic of eukaryotic cells. |
| first_indexed | 2024-04-12T02:09:07Z |
| format | Article |
| id | doaj.art-7b95e734e5284859b509a81c185a0556 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:09:07Z |
| publishDate | 2018-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7b95e734e5284859b509a81c185a05562022-12-22T03:52:27ZengeLife Sciences Publications LtdeLife2050-084X2018-03-01710.7554/eLife.34122A conserved function for pericentromeric satellite DNAMadhav Jagannathan0https://orcid.org/0000-0003-3428-6812Ryan Cummings1https://orcid.org/0000-0003-0540-9174Yukiko M Yamashita2https://orcid.org/0000-0001-5541-0216Life Sciences Institute, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United States; Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United StatesLife Sciences Institute, University of Michigan, Ann Arbor, United States; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, United States; Howard Hughes Medical Institute, University of Michigan, Ann Arbor, United StatesA universal and unquestioned characteristic of eukaryotic cells is that the genome is divided into multiple chromosomes and encapsulated in a single nucleus. However, the underlying mechanism to ensure such a configuration is unknown. Here, we provide evidence that pericentromeric satellite DNA, which is often regarded as junk, is a critical constituent of the chromosome, allowing the packaging of all chromosomes into a single nucleus. We show that the multi-AT-hook satellite DNA-binding proteins, Drosophila melanogaster D1 and mouse HMGA1, play an evolutionarily conserved role in bundling pericentromeric satellite DNA from heterologous chromosomes into ‘chromocenters’, a cytological association of pericentromeric heterochromatin. Defective chromocenter formation leads to micronuclei formation due to budding from the interphase nucleus, DNA damage and cell death. We propose that chromocenter and satellite DNA serve a fundamental role in encapsulating the full complement of the genome within a single nucleus, the universal characteristic of eukaryotic cells.https://elifesciences.org/articles/34122Satellite DNAchromocentermicronucleipericentromeric heterochromatin |
| spellingShingle | Madhav Jagannathan Ryan Cummings Yukiko M Yamashita A conserved function for pericentromeric satellite DNA eLife Satellite DNA chromocenter micronuclei pericentromeric heterochromatin |
| title | A conserved function for pericentromeric satellite DNA |
| title_full | A conserved function for pericentromeric satellite DNA |
| title_fullStr | A conserved function for pericentromeric satellite DNA |
| title_full_unstemmed | A conserved function for pericentromeric satellite DNA |
| title_short | A conserved function for pericentromeric satellite DNA |
| title_sort | conserved function for pericentromeric satellite dna |
| topic | Satellite DNA chromocenter micronuclei pericentromeric heterochromatin |
| url | https://elifesciences.org/articles/34122 |
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