HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines
Transgenic lines of grapevine containing silencing constructs of F3´5´H based on ihpRNA mechanism were studied for their flavonoids and for the expression of F3´5´H gene copies. 83-89 % of transgenic lines showed various degrees of silencing, and increase of di-hydroxylated anthocyanins and kaempfer...
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| Language: | deu |
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Julius Kühn-Institut
2016-07-01
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| Series: | Julius-Kühn-Archiv |
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| Online Access: | http://pub.jki.bund.de/index.php/JKA/article/view/6502/6101 |
| _version_ | 1828496415326732288 |
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| author | Pezhmanmehr, Maryam Moosavi, Amir Ebadi, Ali McDavid, Debra Walker, Amanda R. |
| author_facet | Pezhmanmehr, Maryam Moosavi, Amir Ebadi, Ali McDavid, Debra Walker, Amanda R. |
| author_sort | Pezhmanmehr, Maryam |
| collection | DOAJ |
| description | Transgenic lines of grapevine containing silencing constructs of F3´5´H based on ihpRNA mechanism were studied for their flavonoids and for the expression of F3´5´H gene copies. 83-89 % of transgenic lines showed various degrees of silencing, and increase of di-hydroxylated anthocyanins and kaempferol were observed in the leaves of transgenic lines. |
| first_indexed | 2024-12-11T12:29:22Z |
| format | Article |
| id | doaj.art-7b97436dcf654400b755a61e2ec0705f |
| institution | Directory Open Access Journal |
| issn | 1868-9892 1868-9892 |
| language | deu |
| last_indexed | 2024-12-11T12:29:22Z |
| publishDate | 2016-07-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Julius-Kühn-Archiv |
| spelling | doaj.art-7b97436dcf654400b755a61e2ec0705f2022-12-22T01:07:17ZdeuJulius Kühn-InstitutJulius-Kühn-Archiv1868-98921868-98922016-07-01453939610.5073/jka.2016.453.031HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic linesPezhmanmehr, Maryam 0Moosavi, Amir 1Ebadi, Ali 2McDavid, Debra 3Walker, Amanda R. 4Department of Horticulture, Science and Research Branch, Islamic Azad University, Tehran, IranNational Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, IranDepartment of Horticultural and Landscape Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, IranCSIRO Plant Industries, Adelaide, PO Box 350, Glen Osmond, SA 5064, AustraliaCSIRO Plant Industries, Adelaide, PO Box 350, Glen Osmond, SA 5064, AustraliaTransgenic lines of grapevine containing silencing constructs of F3´5´H based on ihpRNA mechanism were studied for their flavonoids and for the expression of F3´5´H gene copies. 83-89 % of transgenic lines showed various degrees of silencing, and increase of di-hydroxylated anthocyanins and kaempferol were observed in the leaves of transgenic lines.http://pub.jki.bund.de/index.php/JKA/article/view/6502/6101Vitis viniferaanthocyaninflavonolF3'5'Hsilencing |
| spellingShingle | Pezhmanmehr, Maryam Moosavi, Amir Ebadi, Ali McDavid, Debra Walker, Amanda R. HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines Julius-Kühn-Archiv Vitis vinifera anthocyanin flavonol F3'5'H silencing |
| title | HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines |
| title_full | HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines |
| title_fullStr | HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines |
| title_full_unstemmed | HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines |
| title_short | HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines |
| title_sort | hplc analysis of anthocyanins and flavonols and expressions of different copies of f3´5´h in grapevine transgenic lines |
| topic | Vitis vinifera anthocyanin flavonol F3'5'H silencing |
| url | http://pub.jki.bund.de/index.php/JKA/article/view/6502/6101 |
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