Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes
Transgenic Nipponbare which over-expressed a Na+/H+ antiporter gene OsNHX1 was used to compare its growth performance, water status and photosynthetic efficiency with its wild type under varying salinity regimes. Chlorophyll content, quantum yield and photosynthetic rate were measured to assess the...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-11-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630815000694 |
| _version_ | 1818669462571712512 |
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| author | Nurul Kahrani ISHAK Zohrah SULAIMAN Kushan U. TENNAKOON |
| author_facet | Nurul Kahrani ISHAK Zohrah SULAIMAN Kushan U. TENNAKOON |
| author_sort | Nurul Kahrani ISHAK |
| collection | DOAJ |
| description | Transgenic Nipponbare which over-expressed a Na+/H+ antiporter gene OsNHX1 was used to compare its growth performance, water status and photosynthetic efficiency with its wild type under varying salinity regimes. Chlorophyll content, quantum yield and photosynthetic rate were measured to assess the impact of salinity stress on photosynthetic efficiency for transgenic and wild-type Nipponbare. Effects of salinity on water status and gas exchange to both lines were studied by measuring water use efficiency, instantaneous transpiration rate and stomatal conductance. Dry shoot weight and leaf area were determined after three months of growth to assess the impacts of salinity on the growth of those two lines. Our study showed that both lines were affected by salinity stress, however, the transgenic line showed higher photosynthetic efficiency, better utilization of water, and better growth due to low transpiration rate and stomatal conductance. Reduction of photosynthetic efficiency exhibited by the wild-type Nipponbare was correlated to its poor growth under salinity stress. |
| first_indexed | 2024-12-17T06:52:36Z |
| format | Article |
| id | doaj.art-7b9939380a7a4cd28887295cecc98636 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-12-17T06:52:36Z |
| publishDate | 2015-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-7b9939380a7a4cd28887295cecc986362022-12-21T21:59:33ZengElsevierRice Science1672-63082015-11-0122627528210.1016/j.rsci.2015.06.002Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity RegimesNurul Kahrani ISHAK0Zohrah SULAIMAN1Kushan U. TENNAKOON2Environmental and Life Sciences, Faculty of Science, University Brunei Darussalam, Jln Tungku Link, Gadong BE 1410, Brunei DarussalamEnvironmental and Life Sciences, Faculty of Science, University Brunei Darussalam, Jln Tungku Link, Gadong BE 1410, Brunei DarussalamEnvironmental and Life Sciences, Faculty of Science, University Brunei Darussalam, Jln Tungku Link, Gadong BE 1410, Brunei DarussalamTransgenic Nipponbare which over-expressed a Na+/H+ antiporter gene OsNHX1 was used to compare its growth performance, water status and photosynthetic efficiency with its wild type under varying salinity regimes. Chlorophyll content, quantum yield and photosynthetic rate were measured to assess the impact of salinity stress on photosynthetic efficiency for transgenic and wild-type Nipponbare. Effects of salinity on water status and gas exchange to both lines were studied by measuring water use efficiency, instantaneous transpiration rate and stomatal conductance. Dry shoot weight and leaf area were determined after three months of growth to assess the impacts of salinity on the growth of those two lines. Our study showed that both lines were affected by salinity stress, however, the transgenic line showed higher photosynthetic efficiency, better utilization of water, and better growth due to low transpiration rate and stomatal conductance. Reduction of photosynthetic efficiency exhibited by the wild-type Nipponbare was correlated to its poor growth under salinity stress.http://www.sciencedirect.com/science/article/pii/S1672630815000694growth performancesalinity stressNa+/H+ antiporter gene OsNHX1transgenic ricephotosynthetic efficiencywater status |
| spellingShingle | Nurul Kahrani ISHAK Zohrah SULAIMAN Kushan U. TENNAKOON Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes Rice Science growth performance salinity stress Na+/H+ antiporter gene OsNHX1 transgenic rice photosynthetic efficiency water status |
| title | Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes |
| title_full | Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes |
| title_fullStr | Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes |
| title_full_unstemmed | Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes |
| title_short | Comparative Study on Growth Performance of Transgenic (Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes |
| title_sort | comparative study on growth performance of transgenic over expressed osnhx1 and wild type nipponbare under different salinity regimes |
| topic | growth performance salinity stress Na+/H+ antiporter gene OsNHX1 transgenic rice photosynthetic efficiency water status |
| url | http://www.sciencedirect.com/science/article/pii/S1672630815000694 |
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