Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i>
Heat and drought stresses are increasingly relevant topics in the context of climate change, particularly in the Alps, which are warming faster than the global average. Previously, we have shown that alpine plants, including <i>Primula minima</i>, can be gradually heat hardened under fie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/5/1093 |
| _version_ | 1827742277696487424 |
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| author | Thomas Roach Gilbert Neuner Ilse Kranner Othmar Buchner |
| author_facet | Thomas Roach Gilbert Neuner Ilse Kranner Othmar Buchner |
| author_sort | Thomas Roach |
| collection | DOAJ |
| description | Heat and drought stresses are increasingly relevant topics in the context of climate change, particularly in the Alps, which are warming faster than the global average. Previously, we have shown that alpine plants, including <i>Primula minima</i>, can be gradually heat hardened under field conditions in situ to achieve maximum tolerance within a week. Here, we investigated the antioxidant mechanisms of <i>P. minima</i> leaves that had been heat hardened (H) without or with (H+D) additional drought stress. Lower free-radical scavenging and ascorbate concentrations were found in H and H+D leaves, while concentrations of glutathione disulphide (GSSG) were higher under both treatments without any change in glutathione (GSH) and little change in glutathione reductase activity. In contrast, ascorbate peroxidase activity in H leaves was increased, and H+D leaves had >two-fold higher catalase, ascorbate peroxidase and glucose-6-phosphate dehydrogenase activities compared with the control. In addition, the glutathione reductase activity was higher in H+D compared with H leaves. Our results highlight that the stress load from heat acclimation to maximum tolerance is associated with a weakened low-molecular-weight antioxidant defence, which may be compensated for by an increased activity of antioxidant enzymes, particularly under drought conditions. |
| first_indexed | 2024-03-11T03:59:39Z |
| format | Article |
| id | doaj.art-7b9996964960447cad30d27beb1e52e5 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T03:59:39Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7b9996964960447cad30d27beb1e52e52023-11-18T00:15:29ZengMDPI AGAntioxidants2076-39212023-05-01125109310.3390/antiox12051093Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i>Thomas Roach0Gilbert Neuner1Ilse Kranner2Othmar Buchner3Department of Botany, University of Innsbruck, Sternwartestrasse 15, 6020 Innsbruck, AustriaDepartment of Botany, University of Innsbruck, Sternwartestrasse 15, 6020 Innsbruck, AustriaDepartment of Botany, University of Innsbruck, Sternwartestrasse 15, 6020 Innsbruck, AustriaDepartment of Botany, University of Innsbruck, Sternwartestrasse 15, 6020 Innsbruck, AustriaHeat and drought stresses are increasingly relevant topics in the context of climate change, particularly in the Alps, which are warming faster than the global average. Previously, we have shown that alpine plants, including <i>Primula minima</i>, can be gradually heat hardened under field conditions in situ to achieve maximum tolerance within a week. Here, we investigated the antioxidant mechanisms of <i>P. minima</i> leaves that had been heat hardened (H) without or with (H+D) additional drought stress. Lower free-radical scavenging and ascorbate concentrations were found in H and H+D leaves, while concentrations of glutathione disulphide (GSSG) were higher under both treatments without any change in glutathione (GSH) and little change in glutathione reductase activity. In contrast, ascorbate peroxidase activity in H leaves was increased, and H+D leaves had >two-fold higher catalase, ascorbate peroxidase and glucose-6-phosphate dehydrogenase activities compared with the control. In addition, the glutathione reductase activity was higher in H+D compared with H leaves. Our results highlight that the stress load from heat acclimation to maximum tolerance is associated with a weakened low-molecular-weight antioxidant defence, which may be compensated for by an increased activity of antioxidant enzymes, particularly under drought conditions.https://www.mdpi.com/2076-3921/12/5/1093heat tolerancedrought stressreactive oxygen speciesclimate changeacclimationantioxidant |
| spellingShingle | Thomas Roach Gilbert Neuner Ilse Kranner Othmar Buchner Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i> Antioxidants heat tolerance drought stress reactive oxygen species climate change acclimation antioxidant |
| title | Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i> |
| title_full | Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i> |
| title_fullStr | Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i> |
| title_full_unstemmed | Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i> |
| title_short | Heat Acclimation under Drought Stress Induces Antioxidant Enzyme Activity in the Alpine Plant <i>Primula minima</i> |
| title_sort | heat acclimation under drought stress induces antioxidant enzyme activity in the alpine plant i primula minima i |
| topic | heat tolerance drought stress reactive oxygen species climate change acclimation antioxidant |
| url | https://www.mdpi.com/2076-3921/12/5/1093 |
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