Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or Clotrimazole
Aim: The ability of antifungal agents to reduce or eliminate biofilm formation by Candida albicans after incorporating heat-cured acrylic denture base materials was investigated. Materials and Methods: Different concentrations of Amphotericin B (AmB) and Clotrimazole (CT) were incorporated into poly...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Journal of Oral Research and Review |
| Subjects: | |
| Online Access: | http://www.jorr.org/article.asp?issn=2249-4987;year=2018;volume=10;issue=2;spage=51;epage=56;aulast=S. |
| _version_ | 1830221909871034368 |
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| author | Ali Abdul Hussein S. AL-Janabi Makarem M Abdulkareem |
| author_facet | Ali Abdul Hussein S. AL-Janabi Makarem M Abdulkareem |
| author_sort | Ali Abdul Hussein S. AL-Janabi |
| collection | DOAJ |
| description | Aim: The ability of antifungal agents to reduce or eliminate biofilm formation by Candida albicans after incorporating heat-cured acrylic denture base materials was investigated.
Materials and Methods: Different concentrations of Amphotericin B (AmB) and Clotrimazole (CT) were incorporated into polymethylmethacrylate (PMMA) specimens (10 mm × 10 mm × 2 mm). C. albicans was stimulated to produce biofilms on the surface of specimens. Biofilm count was determined by crystal violet and transmittance percentage assays. Porosity percentage of PMMA specimens was measured.
Results: AmB and CT showed more effective action against C. albicans biofilms at low concentrations (1, 5, and 10 μg/ml). Meanwhile, high concentrations (25 and 50 μg/ml) showed less activity. Porosity percentage was decreased in PMMA containing low concentrations of both antifungal agents.
Conclusion: Incorporation of AmB and CT into denture materials has a significant inhibitory effect on the biofilm produced by C. albicans, especially at low concentrations. Decrease in porosity level is another advantage evidenced by incorporating low concentrations of AmB and CT within denture. |
| first_indexed | 2024-12-18T08:33:56Z |
| format | Article |
| id | doaj.art-7b9a1468d8e14879b627e68a28ba35aa |
| institution | Directory Open Access Journal |
| issn | 2249-4987 2394-2541 |
| language | English |
| last_indexed | 2024-12-18T08:33:56Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Oral Research and Review |
| spelling | doaj.art-7b9a1468d8e14879b627e68a28ba35aa2022-12-21T21:14:22ZengWolters Kluwer Medknow PublicationsJournal of Oral Research and Review2249-49872394-25412018-01-01102515610.4103/jorr.jorr_36_17Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or ClotrimazoleAli Abdul Hussein S. AL-JanabiMakarem M AbdulkareemAim: The ability of antifungal agents to reduce or eliminate biofilm formation by Candida albicans after incorporating heat-cured acrylic denture base materials was investigated. Materials and Methods: Different concentrations of Amphotericin B (AmB) and Clotrimazole (CT) were incorporated into polymethylmethacrylate (PMMA) specimens (10 mm × 10 mm × 2 mm). C. albicans was stimulated to produce biofilms on the surface of specimens. Biofilm count was determined by crystal violet and transmittance percentage assays. Porosity percentage of PMMA specimens was measured. Results: AmB and CT showed more effective action against C. albicans biofilms at low concentrations (1, 5, and 10 μg/ml). Meanwhile, high concentrations (25 and 50 μg/ml) showed less activity. Porosity percentage was decreased in PMMA containing low concentrations of both antifungal agents. Conclusion: Incorporation of AmB and CT into denture materials has a significant inhibitory effect on the biofilm produced by C. albicans, especially at low concentrations. Decrease in porosity level is another advantage evidenced by incorporating low concentrations of AmB and CT within denture.http://www.jorr.org/article.asp?issn=2249-4987;year=2018;volume=10;issue=2;spage=51;epage=56;aulast=S.Amphotericin BcandidiasisClotrimazoledenture |
| spellingShingle | Ali Abdul Hussein S. AL-Janabi Makarem M Abdulkareem Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or Clotrimazole Journal of Oral Research and Review Amphotericin B candidiasis Clotrimazole denture |
| title | Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or Clotrimazole |
| title_full | Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or Clotrimazole |
| title_fullStr | Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or Clotrimazole |
| title_full_unstemmed | Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or Clotrimazole |
| title_short | Elevation of the resistance of heat-cured acrylic denture base resin against biofilm-forming Candida albicans by incorporating Amphotericin B or Clotrimazole |
| title_sort | elevation of the resistance of heat cured acrylic denture base resin against biofilm forming candida albicans by incorporating amphotericin b or clotrimazole |
| topic | Amphotericin B candidiasis Clotrimazole denture |
| url | http://www.jorr.org/article.asp?issn=2249-4987;year=2018;volume=10;issue=2;spage=51;epage=56;aulast=S. |
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