Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice Operations
<i>Listeria monocytogenes</i> is a major foodborne pathogen that can contaminate food products and colonize food-producing facilities. Foodservice operations (FSOp) are frequently responsible for foodborne outbreaks due to food safety practices failures. We investigated the presence of a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/6/886 |
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| author | Magaly Toro Jessica Williams-Vergara Camila Solar Ana María Quesille-Villalobos Hee Jin Kwon Paola Navarrete Jianghong Meng Yi Chen Angélica Reyes-Jara |
| author_facet | Magaly Toro Jessica Williams-Vergara Camila Solar Ana María Quesille-Villalobos Hee Jin Kwon Paola Navarrete Jianghong Meng Yi Chen Angélica Reyes-Jara |
| author_sort | Magaly Toro |
| collection | DOAJ |
| description | <i>Listeria monocytogenes</i> is a major foodborne pathogen that can contaminate food products and colonize food-producing facilities. Foodservice operations (FSOp) are frequently responsible for foodborne outbreaks due to food safety practices failures. We investigated the presence of and characterized <i>L. monocytogenes</i> from two FSOp (cafeterias) distributing ready-to-eat meals and verified FSOp’s compliance with good manufacturing practices (GMP). Two facilities (FSOp-A and FSOp-B) were visited three times each over 5 months. We sampled foods, ingredients, and surfaces for microbiological analysis, and <i>L. monocytogenes</i> isolates were characterized by phylogenetic analyses and phenotypic characteristics. GMP audits were performed in the first and third visits. A ready-to-eat salad (FSOp-A) and a frozen ingredient (FSOp-B) were contaminated with <i>L. monocytogenes,</i> which was also detected on Zone 3 surfaces (floor, drains, and a boot cover). The phylogenetic analysis demonstrated that FSOp-B had persistent <i>L. monocytogenes</i> strains, but environmental isolates were not closely related to food or ingredient isolates. GMP audits showed that both operations worked under “fair” conditions, and “facilities and equipment” was the section with the least compliances. The presence of <i>L. monocytogenes</i> in the environment and GMP failures could promote food contamination with this pathogen, presenting a risk to consumers. |
| first_indexed | 2024-03-09T19:50:41Z |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T19:50:41Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-7b9b45f7fa0a47e0b03d834543774b502023-11-24T01:11:28ZengMDPI AGFoods2304-81582022-03-0111688610.3390/foods11060886Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice OperationsMagaly Toro0Jessica Williams-Vergara1Camila Solar2Ana María Quesille-Villalobos3Hee Jin Kwon4Paola Navarrete5Jianghong Meng6Yi Chen7Angélica Reyes-Jara8Laboratorio de Microbiología y Probióticos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Avenida El Líbano 5524, Macul, Santiago 7810000, ChileLaboratorio de Microbiología y Probióticos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Avenida El Líbano 5524, Macul, Santiago 7810000, ChileLaboratorio de Microbiología y Probióticos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Avenida El Líbano 5524, Macul, Santiago 7810000, ChileLaboratorio de Microbiología y Probióticos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Avenida El Líbano 5524, Macul, Santiago 7810000, ChileDepartment of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USALaboratorio de Microbiología y Probióticos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Avenida El Líbano 5524, Macul, Santiago 7810000, ChileDepartment of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USACenter for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USALaboratorio de Microbiología y Probióticos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Avenida El Líbano 5524, Macul, Santiago 7810000, Chile<i>Listeria monocytogenes</i> is a major foodborne pathogen that can contaminate food products and colonize food-producing facilities. Foodservice operations (FSOp) are frequently responsible for foodborne outbreaks due to food safety practices failures. We investigated the presence of and characterized <i>L. monocytogenes</i> from two FSOp (cafeterias) distributing ready-to-eat meals and verified FSOp’s compliance with good manufacturing practices (GMP). Two facilities (FSOp-A and FSOp-B) were visited three times each over 5 months. We sampled foods, ingredients, and surfaces for microbiological analysis, and <i>L. monocytogenes</i> isolates were characterized by phylogenetic analyses and phenotypic characteristics. GMP audits were performed in the first and third visits. A ready-to-eat salad (FSOp-A) and a frozen ingredient (FSOp-B) were contaminated with <i>L. monocytogenes,</i> which was also detected on Zone 3 surfaces (floor, drains, and a boot cover). The phylogenetic analysis demonstrated that FSOp-B had persistent <i>L. monocytogenes</i> strains, but environmental isolates were not closely related to food or ingredient isolates. GMP audits showed that both operations worked under “fair” conditions, and “facilities and equipment” was the section with the least compliances. The presence of <i>L. monocytogenes</i> in the environment and GMP failures could promote food contamination with this pathogen, presenting a risk to consumers.https://www.mdpi.com/2304-8158/11/6/886<i>Listeria monocytogenes</i>foodservice operationsGMPbiofilmquaternary ammonium resistancegenomic analysis |
| spellingShingle | Magaly Toro Jessica Williams-Vergara Camila Solar Ana María Quesille-Villalobos Hee Jin Kwon Paola Navarrete Jianghong Meng Yi Chen Angélica Reyes-Jara Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice Operations Foods <i>Listeria monocytogenes</i> foodservice operations GMP biofilm quaternary ammonium resistance genomic analysis |
| title | Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice Operations |
| title_full | Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice Operations |
| title_fullStr | Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice Operations |
| title_full_unstemmed | Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice Operations |
| title_short | Evaluation of the Persistence and Characterization of <i>Listeria monocytogenes</i> in Foodservice Operations |
| title_sort | evaluation of the persistence and characterization of i listeria monocytogenes i in foodservice operations |
| topic | <i>Listeria monocytogenes</i> foodservice operations GMP biofilm quaternary ammonium resistance genomic analysis |
| url | https://www.mdpi.com/2304-8158/11/6/886 |
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