Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate
Abstract The present study aimed to assess the molecular profiles of subepithelial connective tissue grafts (CTGs) obtained at different locations and depths in the human palate. Sixty-four CTGs belonging to anterior deep (AD), anterior superficial (AS), posterior deep (PD), and posterior superficia...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2023-12-01
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| Series: | International Journal of Oral Science |
| Online Access: | https://doi.org/10.1038/s41368-023-00260-1 |
| _version_ | 1797388479123947520 |
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| author | Maria B. Asparuhova Xiaoqing Song Dominic Riedwyl Geert van Geest Dieter D. Bosshardt Anton Sculean |
| author_facet | Maria B. Asparuhova Xiaoqing Song Dominic Riedwyl Geert van Geest Dieter D. Bosshardt Anton Sculean |
| author_sort | Maria B. Asparuhova |
| collection | DOAJ |
| description | Abstract The present study aimed to assess the molecular profiles of subepithelial connective tissue grafts (CTGs) obtained at different locations and depths in the human palate. Sixty-four CTGs belonging to anterior deep (AD), anterior superficial (AS), posterior deep (PD), and posterior superficial (PS) groups were subjected to RNA-Sequencing and their transcriptomes were analyzed computationally. Functional correlations characterizing the CTG groups were validated by cell biological experiments using primary human palatal fibroblasts (HPFs) extracted from the CTGs. A clearly more pronounced location-dependent than depth-dependent difference between the grafts, with a minimal number of genes (4) showing no dependence on the location, was revealed. Epithelial, endothelial, and monocytic cell migration was strongly (P < 0.001) potentiated by AD- and PS-HPFs. Moreover, significantly increased expression of genes encoding C-C and C-X-C motif chemokine ligands as well as significantly (P < 0.01) activated p38 signaling suggested immunomodulatory phenotype for AD- and PS-HPFs. Increased growth factor gene expression and significantly activated (P < 0.001) Erk and Akt signaling in HPFs originating from A-CTGs implied their involvement in cell survival, proliferation, and motility. Prominent collagen-rich expression profile contributing to high mechanical stability, increased osteogenesis-related gene expression, and strongly activated (P < 0.001) Smad1/5/8 signaling characterized HPFs originating from P-CTGs. The present data indicate that in humans, differences between palatal CTGs harvested from different locations and depths appear to be location- rather than depth-dependent. Our findings provide the basis for future personalization of the therapeutic strategy by selecting an optimal graft type depending on the clinical indications. |
| first_indexed | 2024-03-08T22:41:25Z |
| format | Article |
| id | doaj.art-7ba0f437e1d34f889ed04900bda40668 |
| institution | Directory Open Access Journal |
| issn | 2049-3169 |
| language | English |
| last_indexed | 2024-03-08T22:41:25Z |
| publishDate | 2023-12-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | International Journal of Oral Science |
| spelling | doaj.art-7ba0f437e1d34f889ed04900bda406682023-12-17T12:08:58ZengNature Publishing GroupInternational Journal of Oral Science2049-31692023-12-0115111710.1038/s41368-023-00260-1Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palateMaria B. Asparuhova0Xiaoqing Song1Dominic Riedwyl2Geert van Geest3Dieter D. Bosshardt4Anton Sculean5Laboratory of Oral Cell Biology, School of Dental Medicine, University of BernLaboratory of Oral Cell Biology, School of Dental Medicine, University of BernLaboratory of Oral Cell Biology, School of Dental Medicine, University of BernInterfaculty Bioinformatics Unit, University of BernDepartment of Periodontology, School of Dental Medicine, University of BernDepartment of Periodontology, School of Dental Medicine, University of BernAbstract The present study aimed to assess the molecular profiles of subepithelial connective tissue grafts (CTGs) obtained at different locations and depths in the human palate. Sixty-four CTGs belonging to anterior deep (AD), anterior superficial (AS), posterior deep (PD), and posterior superficial (PS) groups were subjected to RNA-Sequencing and their transcriptomes were analyzed computationally. Functional correlations characterizing the CTG groups were validated by cell biological experiments using primary human palatal fibroblasts (HPFs) extracted from the CTGs. A clearly more pronounced location-dependent than depth-dependent difference between the grafts, with a minimal number of genes (4) showing no dependence on the location, was revealed. Epithelial, endothelial, and monocytic cell migration was strongly (P < 0.001) potentiated by AD- and PS-HPFs. Moreover, significantly increased expression of genes encoding C-C and C-X-C motif chemokine ligands as well as significantly (P < 0.01) activated p38 signaling suggested immunomodulatory phenotype for AD- and PS-HPFs. Increased growth factor gene expression and significantly activated (P < 0.001) Erk and Akt signaling in HPFs originating from A-CTGs implied their involvement in cell survival, proliferation, and motility. Prominent collagen-rich expression profile contributing to high mechanical stability, increased osteogenesis-related gene expression, and strongly activated (P < 0.001) Smad1/5/8 signaling characterized HPFs originating from P-CTGs. The present data indicate that in humans, differences between palatal CTGs harvested from different locations and depths appear to be location- rather than depth-dependent. Our findings provide the basis for future personalization of the therapeutic strategy by selecting an optimal graft type depending on the clinical indications.https://doi.org/10.1038/s41368-023-00260-1 |
| spellingShingle | Maria B. Asparuhova Xiaoqing Song Dominic Riedwyl Geert van Geest Dieter D. Bosshardt Anton Sculean Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate International Journal of Oral Science |
| title | Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate |
| title_full | Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate |
| title_fullStr | Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate |
| title_full_unstemmed | Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate |
| title_short | Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate |
| title_sort | differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate |
| url | https://doi.org/10.1038/s41368-023-00260-1 |
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