Molecular Proteomics and Signalling of Human Platelets in Health and Disease
Platelets are small anucleate blood cells that play vital roles in haemostasis and thrombosis, besides other physiological and pathophysiological processes. These roles are tightly regulated by a complex network of signalling pathways. Mass spectrometry-based proteomic techniques are contributing no...
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MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/18/9860 |
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| author | Jingnan Huang Pengyu Zhang Fiorella A. Solari Albert Sickmann Angel Garcia Kerstin Jurk Johan W. M. Heemskerk |
| author_facet | Jingnan Huang Pengyu Zhang Fiorella A. Solari Albert Sickmann Angel Garcia Kerstin Jurk Johan W. M. Heemskerk |
| author_sort | Jingnan Huang |
| collection | DOAJ |
| description | Platelets are small anucleate blood cells that play vital roles in haemostasis and thrombosis, besides other physiological and pathophysiological processes. These roles are tightly regulated by a complex network of signalling pathways. Mass spectrometry-based proteomic techniques are contributing not only to the identification and quantification of new platelet proteins, but also reveal post-translational modifications of these molecules, such as acetylation, glycosylation and phosphorylation. Moreover, target proteomic analysis of platelets can provide molecular biomarkers for genetic aberrations with established or non-established links to platelet dysfunctions. In this report, we review 67 reports regarding platelet proteomic analysis and signalling on a molecular base. Collectively, these provide detailed insight into the: (i) technical developments and limitations of the assessment of platelet (sub)proteomes; (ii) molecular protein changes upon ageing of platelets; (iii) complexity of platelet signalling pathways and functions in response to collagen, rhodocytin, thrombin, thromboxane A<sub>2</sub> and ADP; (iv) proteomic effects of endothelial-derived mediators such as prostacyclin and the anti-platelet drug aspirin; and (v) molecular protein changes in platelets from patients with congenital disorders or cardiovascular disease. However, sample sizes are still low and the roles of differentially expressed proteins are often unknown. Based on the practical and technical possibilities and limitations, we provide a perspective for further improvements of the platelet proteomic field. |
| first_indexed | 2024-03-10T07:35:49Z |
| format | Article |
| id | doaj.art-7bad1169d5b84c1eaf275309c220b028 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T07:35:49Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7bad1169d5b84c1eaf275309c220b0282023-11-22T13:28:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-012218986010.3390/ijms22189860Molecular Proteomics and Signalling of Human Platelets in Health and DiseaseJingnan Huang0Pengyu Zhang1Fiorella A. Solari2Albert Sickmann3Angel Garcia4Kerstin Jurk5Johan W. M. Heemskerk6Leibniz Institut für Analytische Wissenschaften—ISAS-e.V., 44139 Dortmund, GermanyLeibniz Institut für Analytische Wissenschaften—ISAS-e.V., 44139 Dortmund, GermanyLeibniz Institut für Analytische Wissenschaften—ISAS-e.V., 44139 Dortmund, GermanyLeibniz Institut für Analytische Wissenschaften—ISAS-e.V., 44139 Dortmund, GermanyCenter for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela, 15706 Santiago de Compostela, SpainCenter for Thrombosis and Haemostasis, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, GermanyDepartment of Biochemistry, CARIM, Maastricht University, 6229 ER Maastricht, The NetherlandsPlatelets are small anucleate blood cells that play vital roles in haemostasis and thrombosis, besides other physiological and pathophysiological processes. These roles are tightly regulated by a complex network of signalling pathways. Mass spectrometry-based proteomic techniques are contributing not only to the identification and quantification of new platelet proteins, but also reveal post-translational modifications of these molecules, such as acetylation, glycosylation and phosphorylation. Moreover, target proteomic analysis of platelets can provide molecular biomarkers for genetic aberrations with established or non-established links to platelet dysfunctions. In this report, we review 67 reports regarding platelet proteomic analysis and signalling on a molecular base. Collectively, these provide detailed insight into the: (i) technical developments and limitations of the assessment of platelet (sub)proteomes; (ii) molecular protein changes upon ageing of platelets; (iii) complexity of platelet signalling pathways and functions in response to collagen, rhodocytin, thrombin, thromboxane A<sub>2</sub> and ADP; (iv) proteomic effects of endothelial-derived mediators such as prostacyclin and the anti-platelet drug aspirin; and (v) molecular protein changes in platelets from patients with congenital disorders or cardiovascular disease. However, sample sizes are still low and the roles of differentially expressed proteins are often unknown. Based on the practical and technical possibilities and limitations, we provide a perspective for further improvements of the platelet proteomic field.https://www.mdpi.com/1422-0067/22/18/9860plateletspost-translational modificationproteinssignallingreceptors |
| spellingShingle | Jingnan Huang Pengyu Zhang Fiorella A. Solari Albert Sickmann Angel Garcia Kerstin Jurk Johan W. M. Heemskerk Molecular Proteomics and Signalling of Human Platelets in Health and Disease International Journal of Molecular Sciences platelets post-translational modification proteins signalling receptors |
| title | Molecular Proteomics and Signalling of Human Platelets in Health and Disease |
| title_full | Molecular Proteomics and Signalling of Human Platelets in Health and Disease |
| title_fullStr | Molecular Proteomics and Signalling of Human Platelets in Health and Disease |
| title_full_unstemmed | Molecular Proteomics and Signalling of Human Platelets in Health and Disease |
| title_short | Molecular Proteomics and Signalling of Human Platelets in Health and Disease |
| title_sort | molecular proteomics and signalling of human platelets in health and disease |
| topic | platelets post-translational modification proteins signalling receptors |
| url | https://www.mdpi.com/1422-0067/22/18/9860 |
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