Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae Blastospores
The mosquito Aedes aegypti vectors arboviruses such as urban yellow fever, dengue, chikungunya and Zika. Reducing the vector population is still the most effective method to decrease the spread of arboviruses. Fungi are the main natural disease agents of insects. The present study compared the virul...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2019-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/aedes-aegypti-pupae-are-highly-susceptible-to-infection-by-metarhizium-anisopliae-blastospores/ |
| _version_ | 1819000677440946176 |
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| author | Aline Teixeira Carolino Simone Azevedo Gomes Thais Bercot Pontes Teodoro Thalles Cardoso Mattoso Richard Ian Samuels |
| author_facet | Aline Teixeira Carolino Simone Azevedo Gomes Thais Bercot Pontes Teodoro Thalles Cardoso Mattoso Richard Ian Samuels |
| author_sort | Aline Teixeira Carolino |
| collection | DOAJ |
| description | The mosquito Aedes aegypti vectors arboviruses such as urban yellow fever, dengue, chikungunya and Zika. Reducing the vector population is still the most effective method to decrease the spread of arboviruses. Fungi are the main natural disease agents of insects. The present study compared the virulence of conidia and blastospores of the entomopathogenic fungus Metarhizium anisopliae against A. aegypti pupae. The pupae were obtained by collecting eggs using “ovitraps” deployed in an urban environment. M. anisopliae conidia were produced using solid media and blastospores were produced by inoculating conidia in liquid culture. Blastospores were more virulent against pupae than conidia, and no pupae survived twenty-four hours after exposure to this type of propagule. Large quantities of mucilage were produced by the blastospores in the presence of the pupae. Exposure of pupae to conidial suspensions resulted in 57% survival at 24h and 23% at 48h. A proportion of the adults, which emerged from pupae exposed to conidia, succumbed to infection. This is the first study to demonstrates the infection of A. aegypti pupae by the entomopathogenic fungus M. anisopliae, showing that this stage of development can also be targeted by biological control agents. |
| first_indexed | 2024-12-20T22:37:07Z |
| format | Article |
| id | doaj.art-7bad5f9c6cc04b7c96b6a7bc669be889 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-20T22:37:07Z |
| publishDate | 2019-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-7bad5f9c6cc04b7c96b6a7bc669be8892022-12-21T19:24:35ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2019-09-011331629163410.22207/JPAM.13.3.36Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae BlastosporesAline Teixeira Carolino0Simone Azevedo Gomes1Thais Bercot Pontes Teodoro2Thalles Cardoso Mattoso3Richard Ian Samuels41Department of Entomology and Plant Pathology, State University of North Fluminense. Alberto Lamego Avenue, 2000, Parque California, Campos dos Goytacazes, Rio de Janeiro, Brazil.1Department of Entomology and Plant Pathology, State University of North Fluminense. Alberto Lamego Avenue, 2000, Parque California, Campos dos Goytacazes, Rio de Janeiro, Brazil.1Department of Entomology and Plant Pathology, State University of North Fluminense. Alberto Lamego Avenue, 2000, Parque California, Campos dos Goytacazes, Rio de Janeiro, Brazil.2Federal University of Roraima, Department of Crop Science. Boa Vista, Roraima, Brazil.1Department of Entomology and Plant Pathology, State University of North Fluminense. Alberto Lamego Avenue, 2000, Parque California, Campos dos Goytacazes, Rio de Janeiro, Brazil.The mosquito Aedes aegypti vectors arboviruses such as urban yellow fever, dengue, chikungunya and Zika. Reducing the vector population is still the most effective method to decrease the spread of arboviruses. Fungi are the main natural disease agents of insects. The present study compared the virulence of conidia and blastospores of the entomopathogenic fungus Metarhizium anisopliae against A. aegypti pupae. The pupae were obtained by collecting eggs using “ovitraps” deployed in an urban environment. M. anisopliae conidia were produced using solid media and blastospores were produced by inoculating conidia in liquid culture. Blastospores were more virulent against pupae than conidia, and no pupae survived twenty-four hours after exposure to this type of propagule. Large quantities of mucilage were produced by the blastospores in the presence of the pupae. Exposure of pupae to conidial suspensions resulted in 57% survival at 24h and 23% at 48h. A proportion of the adults, which emerged from pupae exposed to conidia, succumbed to infection. This is the first study to demonstrates the infection of A. aegypti pupae by the entomopathogenic fungus M. anisopliae, showing that this stage of development can also be targeted by biological control agents.https://microbiologyjournal.org/aedes-aegypti-pupae-are-highly-susceptible-to-infection-by-metarhizium-anisopliae-blastospores/virulencefungusvectorbiological controlpupadengue |
| spellingShingle | Aline Teixeira Carolino Simone Azevedo Gomes Thais Bercot Pontes Teodoro Thalles Cardoso Mattoso Richard Ian Samuels Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae Blastospores Journal of Pure and Applied Microbiology virulence fungus vector biological control pupa dengue |
| title | Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae Blastospores |
| title_full | Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae Blastospores |
| title_fullStr | Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae Blastospores |
| title_full_unstemmed | Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae Blastospores |
| title_short | Aedes aegypti Pupae are Highly Susceptible to Infection by Metarhizium anisopliae Blastospores |
| title_sort | aedes aegypti pupae are highly susceptible to infection by metarhizium anisopliae blastospores |
| topic | virulence fungus vector biological control pupa dengue |
| url | https://microbiologyjournal.org/aedes-aegypti-pupae-are-highly-susceptible-to-infection-by-metarhizium-anisopliae-blastospores/ |
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