Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions
The adipose tissue stores excess energy in the form of neutral lipids within adipocyte lipid droplets (LDs). The correct function of LDs requires the interaction with other organelles, such as the endoplasmic reticulum (ER) as well as with LD coat-associated proteins, including Rab18, a mediator of...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/24/17177 |
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| author | Jaime López-Alcalá M. Carmen Soler-Vázquez Carmen Tercero-Alcázar Julia Sánchez-Ceinos Rocío Guzmán-Ruiz María M. Malagón Ana Gordon |
| author_facet | Jaime López-Alcalá M. Carmen Soler-Vázquez Carmen Tercero-Alcázar Julia Sánchez-Ceinos Rocío Guzmán-Ruiz María M. Malagón Ana Gordon |
| author_sort | Jaime López-Alcalá |
| collection | DOAJ |
| description | The adipose tissue stores excess energy in the form of neutral lipids within adipocyte lipid droplets (LDs). The correct function of LDs requires the interaction with other organelles, such as the endoplasmic reticulum (ER) as well as with LD coat-associated proteins, including Rab18, a mediator of intracellular lipid trafficking and ER–LD interaction. Although perturbations of the inter-organelle contact sites have been linked to several diseases, such as cancer, no information regarding ER–LD contact sites in dysfunctional adipocytes from the obese adipose tissue has been published to date. Herein, the ER–LD connection and Rab18 distribution at ER–LD contact sites are examined in adipocytes challenged with fibrosis and inflammatory conditions, which represent known hallmarks of the adipose tissue in obesity. Our results show that adipocytes differentiated in fibrotic conditions caused ER fragmentation, the expansion of ER–LD contact sites, and modified Rab18 dynamics. Likewise, adipocytes exposed to inflammatory conditions favored ER–LD contact, Rab18 accumulation in the ER, and Rab18 redistribution to large LDs. Finally, our studies in human adipocytes supported the suggestion that Rab18 transitions to the LD coat from the ER. Taken together, our results suggest that obesity-related pathogenic processes alter the maintenance of ER–LD interactions and interfere with Rab18 trafficking through these contact sites. |
| first_indexed | 2024-03-08T20:41:53Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:41:53Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7bafbd9df23e4c17b29cb743a01557572023-12-22T14:13:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241717710.3390/ijms242417177Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic ConditionsJaime López-Alcalá0M. Carmen Soler-Vázquez1Carmen Tercero-Alcázar2Julia Sánchez-Ceinos3Rocío Guzmán-Ruiz4María M. Malagón5Ana Gordon6Department of Cell Biology, Physiology, and Immunology, Adipobiology Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofia University Hospital, University of Córdoba, 14004 Córdoba, SpainDepartment of Cell Biology, Physiology, and Immunology, Adipobiology Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofia University Hospital, University of Córdoba, 14004 Córdoba, SpainDepartment of Cell Biology, Physiology, and Immunology, Adipobiology Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofia University Hospital, University of Córdoba, 14004 Córdoba, SpainCardiology Unit, Department of Medicine-Solna, Karolinska Institute (KI), Karolinska University Hospital (NKS), 17177 Stockholm, SwedenDepartment of Cell Biology, Physiology, and Immunology, Adipobiology Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofia University Hospital, University of Córdoba, 14004 Córdoba, SpainDepartment of Cell Biology, Physiology, and Immunology, Adipobiology Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofia University Hospital, University of Córdoba, 14004 Córdoba, SpainDepartment of Cell Biology, Physiology, and Immunology, Adipobiology Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Reina Sofia University Hospital, University of Córdoba, 14004 Córdoba, SpainThe adipose tissue stores excess energy in the form of neutral lipids within adipocyte lipid droplets (LDs). The correct function of LDs requires the interaction with other organelles, such as the endoplasmic reticulum (ER) as well as with LD coat-associated proteins, including Rab18, a mediator of intracellular lipid trafficking and ER–LD interaction. Although perturbations of the inter-organelle contact sites have been linked to several diseases, such as cancer, no information regarding ER–LD contact sites in dysfunctional adipocytes from the obese adipose tissue has been published to date. Herein, the ER–LD connection and Rab18 distribution at ER–LD contact sites are examined in adipocytes challenged with fibrosis and inflammatory conditions, which represent known hallmarks of the adipose tissue in obesity. Our results show that adipocytes differentiated in fibrotic conditions caused ER fragmentation, the expansion of ER–LD contact sites, and modified Rab18 dynamics. Likewise, adipocytes exposed to inflammatory conditions favored ER–LD contact, Rab18 accumulation in the ER, and Rab18 redistribution to large LDs. Finally, our studies in human adipocytes supported the suggestion that Rab18 transitions to the LD coat from the ER. Taken together, our results suggest that obesity-related pathogenic processes alter the maintenance of ER–LD interactions and interfere with Rab18 trafficking through these contact sites.https://www.mdpi.com/1422-0067/24/24/17177adipocyteendoplasmic reticulumlipid dropletRab18fibrosisinflammation |
| spellingShingle | Jaime López-Alcalá M. Carmen Soler-Vázquez Carmen Tercero-Alcázar Julia Sánchez-Ceinos Rocío Guzmán-Ruiz María M. Malagón Ana Gordon Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions International Journal of Molecular Sciences adipocyte endoplasmic reticulum lipid droplet Rab18 fibrosis inflammation |
| title | Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions |
| title_full | Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions |
| title_fullStr | Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions |
| title_full_unstemmed | Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions |
| title_short | Rab18 Drift in Lipid Droplet and Endoplasmic Reticulum Interactions of Adipocytes under Obesogenic Conditions |
| title_sort | rab18 drift in lipid droplet and endoplasmic reticulum interactions of adipocytes under obesogenic conditions |
| topic | adipocyte endoplasmic reticulum lipid droplet Rab18 fibrosis inflammation |
| url | https://www.mdpi.com/1422-0067/24/24/17177 |
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