Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay
To support in vivo and in vitro studies of intravascular triglyceride metabolism in mice, we created rat monoclonal antibodies (mAbs) against mouse LPL. Two mAbs, mAbs 23A1 and 31A5, were used to develop a sandwich ELISA for mouse LPL. The detection of mouse LPL by the ELISA was linear in concentrat...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227524000373 |
| _version_ | 1797213462527475712 |
|---|---|
| author | Takao Kimura Kazuya Miyashita Isamu Fukamachi Kumiko Fukamachi Kazumi Ogura Erina Yokoyama Katsuhiko Tsunekawa Takumi Nagasawa Michael Ploug Ye Yang Wenxin Song Stephen G. Young Anne P. Beigneux Katsuyuki Nakajima Masami Murakami |
| author_facet | Takao Kimura Kazuya Miyashita Isamu Fukamachi Kumiko Fukamachi Kazumi Ogura Erina Yokoyama Katsuhiko Tsunekawa Takumi Nagasawa Michael Ploug Ye Yang Wenxin Song Stephen G. Young Anne P. Beigneux Katsuyuki Nakajima Masami Murakami |
| author_sort | Takao Kimura |
| collection | DOAJ |
| description | To support in vivo and in vitro studies of intravascular triglyceride metabolism in mice, we created rat monoclonal antibodies (mAbs) against mouse LPL. Two mAbs, mAbs 23A1 and 31A5, were used to develop a sandwich ELISA for mouse LPL. The detection of mouse LPL by the ELISA was linear in concentrations ranging from 0.31 ng/ml to 20 ng/ml. The sensitivity of the ELISA made it possible to quantify LPL in serum and in both pre-heparin and post-heparin plasma samples (including in grossly lipemic samples). LPL mass and activity levels in the post-heparin plasma were lower in Gpihbp1−/− mice than in wild-type mice. In both groups of mice, LPL mass and activity levels were positively correlated. Our mAb-based sandwich ELISA for mouse LPL will be useful for any investigator who uses mouse models to study LPL-mediated intravascular lipolysis. |
| first_indexed | 2024-04-24T10:58:40Z |
| format | Article |
| id | doaj.art-7bb09355127f4c74a78df72318b9d2fc |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-04-24T10:58:40Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7bb09355127f4c74a78df72318b9d2fc2024-04-12T04:44:14ZengElsevierJournal of Lipid Research0022-22752024-04-01654100532Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assayTakao Kimura0Kazuya Miyashita1Isamu Fukamachi2Kumiko Fukamachi3Kazumi Ogura4Erina Yokoyama5Katsuhiko Tsunekawa6Takumi Nagasawa7Michael Ploug8Ye Yang9Wenxin Song10Stephen G. Young11Anne P. Beigneux12Katsuyuki Nakajima13Masami Murakami14Department of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan; Clinical Laboratory Center, Gunma University Hospital, Maebashi, Gunma, Japan; For correspondence: Takao KimuraImmuno–Biological Laboratories, Fujioka, Gunma, JapanImmuno–Biological Laboratories, Fujioka, Gunma, JapanImmuno–Biological Laboratories, Fujioka, Gunma, JapanImmuno–Biological Laboratories, Fujioka, Gunma, JapanImmuno–Biological Laboratories, Fujioka, Gunma, JapanDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan; Clinical Laboratory Center, Gunma University Hospital, Maebashi, Gunma, JapanDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan; Clinical Laboratory Center, Gunma University Hospital, Maebashi, Gunma, JapanFinsen Laboratory, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark; Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, DenmarkDepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USADepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USADepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA; Department of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA, USADepartment of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USADepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, JapanDepartment of Clinical Laboratory Medicine, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan; Clinical Laboratory Center, Gunma University Hospital, Maebashi, Gunma, JapanTo support in vivo and in vitro studies of intravascular triglyceride metabolism in mice, we created rat monoclonal antibodies (mAbs) against mouse LPL. Two mAbs, mAbs 23A1 and 31A5, were used to develop a sandwich ELISA for mouse LPL. The detection of mouse LPL by the ELISA was linear in concentrations ranging from 0.31 ng/ml to 20 ng/ml. The sensitivity of the ELISA made it possible to quantify LPL in serum and in both pre-heparin and post-heparin plasma samples (including in grossly lipemic samples). LPL mass and activity levels in the post-heparin plasma were lower in Gpihbp1−/− mice than in wild-type mice. In both groups of mice, LPL mass and activity levels were positively correlated. Our mAb-based sandwich ELISA for mouse LPL will be useful for any investigator who uses mouse models to study LPL-mediated intravascular lipolysis.http://www.sciencedirect.com/science/article/pii/S0022227524000373lipidstriglyceridestransportvascular biologyLPLHTGL |
| spellingShingle | Takao Kimura Kazuya Miyashita Isamu Fukamachi Kumiko Fukamachi Kazumi Ogura Erina Yokoyama Katsuhiko Tsunekawa Takumi Nagasawa Michael Ploug Ye Yang Wenxin Song Stephen G. Young Anne P. Beigneux Katsuyuki Nakajima Masami Murakami Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay Journal of Lipid Research lipids triglycerides transport vascular biology LPL HTGL |
| title | Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay |
| title_full | Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay |
| title_fullStr | Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay |
| title_full_unstemmed | Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay |
| title_short | Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay |
| title_sort | quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme linked immunosorbent assay |
| topic | lipids triglycerides transport vascular biology LPL HTGL |
| url | http://www.sciencedirect.com/science/article/pii/S0022227524000373 |
| work_keys_str_mv | AT takaokimura quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT kazuyamiyashita quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT isamufukamachi quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT kumikofukamachi quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT kazumiogura quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT erinayokoyama quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT katsuhikotsunekawa quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT takuminagasawa quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT michaelploug quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT yeyang quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT wenxinsong quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT stephengyoung quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT annepbeigneux quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT katsuyukinakajima quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay AT masamimurakami quantificationoflipoproteinlipaseinmouseplasmawithasandwichenzymelinkedimmunosorbentassay |