Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers
‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2012-10-01
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| Series: | Acta Agriculturae Slovenica |
| Subjects: | |
| Online Access: | https://journals.uni-lj.si/aas/article/view/14480 |
| _version_ | 1826532298576625664 |
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| author | Lidija TOMIĆ Nataša ŠTAJNER Tatjana JOVANOVIĆ CVETKOVIĆ Miljan CVETKOVIĆ Branka JAVORNIK |
| author_facet | Lidija TOMIĆ Nataša ŠTAJNER Tatjana JOVANOVIĆ CVETKOVIĆ Miljan CVETKOVIĆ Branka JAVORNIK |
| author_sort | Lidija TOMIĆ |
| collection | DOAJ |
| description | ‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use. |
| first_indexed | 2024-03-08T23:23:53Z |
| format | Article |
| id | doaj.art-7bb282a6b24946c8956d853336326fed |
| institution | Directory Open Access Journal |
| issn | 1854-1941 |
| language | English |
| last_indexed | 2025-03-14T01:49:01Z |
| publishDate | 2012-10-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj.art-7bb282a6b24946c8956d853336326fed2025-03-12T12:21:37ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412012-10-0199214315010.14720/aas.2012.99.2.1448020872Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markersLidija TOMIĆ0Nataša ŠTAJNER1Tatjana JOVANOVIĆ CVETKOVIĆ2Miljan CVETKOVIĆ3Branka JAVORNIK4University of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and HerzegovinaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, SloveniaUniversity of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and HerzegovinaUniversity of Banjaluka, Faculty of Agriculture, Bulevar vojvode Petra Bojovića 1A, 78 000 Banjaluka, Bosnia and HerzegovinaUniversity of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1111 Ljubljana, Slovenia‘Žilavka’ has been grown in Bosnia and Herzegovina since the XIVth century and is exploited for wine production. Although not sufficiently studied, this grapevine cultivar has high economic potential for the country. Five survey missions resulted in the collection of eighty ‘Žilavka’ accessions that varied in terms of names and characteristics. Because of the unknown origin of the cultivar, these accessions were analyzed with microsatellites markers in order to obtain a standard ‘Žilavka’ genotype. AFLP markers were used to investigate the genetic basis of variability within the cultivar. ‘Žilavka’ grapevines were screened on 14 microsatellite loci, thus revealing 4 different genotypes arising from mutations observed at 10 polymorphic loci. AFLP analysis of 52 ‘Žilavka’ accessions revealed 35 different genotypes, with an average polymorphism of 57 %. Cluster analysis showed no grouping of different Žilavka accessions according to their names, characteristics or collection locations. The standard ‘Žilavka’ genotype was further compared to 211 cultivars from Slovenia (49), Austria/Germany (20), France (13), Portugal (27), Croatia (19), Greece (32), Spain (21) and Italy (30) in order to assess their genetic relationships. In pairwise comparisons, the highest genetic similarity was found with Slovenian cultivars ‘Glera’ and ‘Briška Glera’ (64 %) and the highest genetic dissimilarity (100 %) with two Italian cultivars, ‘Nebbiolo Lampia’ and ‘Vespolina’. Inventory, collection and genetic characterization of ‘Žilavka’ accessions are important steps towards cultivar standardization, identification of parental cultivars and investigation of cultivar origin, required for its sustainable use.https://journals.uni-lj.si/aas/article/view/14480grapevinemicrosatellitestandard genotypevariabilityaflp |
| spellingShingle | Lidija TOMIĆ Nataša ŠTAJNER Tatjana JOVANOVIĆ CVETKOVIĆ Miljan CVETKOVIĆ Branka JAVORNIK Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers Acta Agriculturae Slovenica grapevine microsatellite standard genotype variability aflp |
| title | Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers |
| title_full | Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers |
| title_fullStr | Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers |
| title_full_unstemmed | Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers |
| title_short | Collection and genetic characterization of Vitis vinifera ’ Žilavka’ by microsatellites and AFLP markers |
| title_sort | collection and genetic characterization of vitis vinifera zilavka by microsatellites and aflp markers |
| topic | grapevine microsatellite standard genotype variability aflp |
| url | https://journals.uni-lj.si/aas/article/view/14480 |
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