CRISPR-Cas effector specificity and cleavage site determine phage escape outcomes.
CRISPR-mediated interference relies on complementarity between a guiding CRISPR RNA (crRNA) and target nucleic acids to provide defense against bacteriophage. Phages escape CRISPR-based immunity mainly through mutations in the protospacer adjacent motif (PAM) and seed regions. However, previous spec...
Main Authors: | Michael A Schelling, Giang T Nguyen, Dipali G Sashital |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2023-04-01
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Series: | PLoS Biology |
Online Access: | https://doi.org/10.1371/journal.pbio.3002065 |
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