Study on the biological effects of ZnO nanosheets on EBL cells
In this study, the biological effects of ZnO nanosheets were initially investigated using embryonic bovine lung (EBL) cells cultured in vitro as a model. ZnO nanosheets were prepared by a hydrothermal method, and their structure and morphology were characterized, and their effects on EBL cell viabil...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.915749/full |
| _version_ | 1811242771154993152 |
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| author | Mei Li Yonghua Ma Xiaodi Lian Yan Lu Yuanyuan Li Yao Xi Xiaolin Sun |
| author_facet | Mei Li Yonghua Ma Xiaodi Lian Yan Lu Yuanyuan Li Yao Xi Xiaolin Sun |
| author_sort | Mei Li |
| collection | DOAJ |
| description | In this study, the biological effects of ZnO nanosheets were initially investigated using embryonic bovine lung (EBL) cells cultured in vitro as a model. ZnO nanosheets were prepared by a hydrothermal method, and their structure and morphology were characterized, and their effects on EBL cell viability, oxidative stress, cell proliferation, and apoptosis were investigated. The results showed that 12.5 μg ml−1 ZnO nanosheets can cause morphological changes in EBL cells. The toxic effects of ZnO nanosheets on EBL cells were time-dependent. Caspase-3 activity in EBL cells changed under certain conditions with the introduction of 25 μg ml−1 ZnO nanomaterials, and EBL cell apoptosis was promoted. Under different concentration and time effects, ZnO nanosheets induced an increase in ROS levels in EBL cells, indicating that they have an oxidative damage effect on cells. The toxic effects of ZnO nanosheets on EBL cells were discussed, including concentration effect, time effect, and cytotoxic effect, which eventually led to cell oxidative damage. |
| first_indexed | 2024-04-12T13:55:59Z |
| format | Article |
| id | doaj.art-7bb666adb74a405cbe57a98cc9cdf83f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-12T13:55:59Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-7bb666adb74a405cbe57a98cc9cdf83f2022-12-22T03:30:22ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-10-011010.3389/fbioe.2022.915749915749Study on the biological effects of ZnO nanosheets on EBL cellsMei Li0Yonghua Ma1Xiaodi Lian2Yan Lu3Yuanyuan Li4Yao Xi5Xiaolin Sun6College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaLanzhou Institute of Biological Products Limited Liability Company, Lanzhou, ChinaNorthwest Normal University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, ChinaIn this study, the biological effects of ZnO nanosheets were initially investigated using embryonic bovine lung (EBL) cells cultured in vitro as a model. ZnO nanosheets were prepared by a hydrothermal method, and their structure and morphology were characterized, and their effects on EBL cell viability, oxidative stress, cell proliferation, and apoptosis were investigated. The results showed that 12.5 μg ml−1 ZnO nanosheets can cause morphological changes in EBL cells. The toxic effects of ZnO nanosheets on EBL cells were time-dependent. Caspase-3 activity in EBL cells changed under certain conditions with the introduction of 25 μg ml−1 ZnO nanomaterials, and EBL cell apoptosis was promoted. Under different concentration and time effects, ZnO nanosheets induced an increase in ROS levels in EBL cells, indicating that they have an oxidative damage effect on cells. The toxic effects of ZnO nanosheets on EBL cells were discussed, including concentration effect, time effect, and cytotoxic effect, which eventually led to cell oxidative damage.https://www.frontiersin.org/articles/10.3389/fbioe.2022.915749/fullnanomaterialsEBL cellscytotoxicityapoptosisreactive oxygen species |
| spellingShingle | Mei Li Yonghua Ma Xiaodi Lian Yan Lu Yuanyuan Li Yao Xi Xiaolin Sun Study on the biological effects of ZnO nanosheets on EBL cells Frontiers in Bioengineering and Biotechnology nanomaterials EBL cells cytotoxicity apoptosis reactive oxygen species |
| title | Study on the biological effects of ZnO nanosheets on EBL cells |
| title_full | Study on the biological effects of ZnO nanosheets on EBL cells |
| title_fullStr | Study on the biological effects of ZnO nanosheets on EBL cells |
| title_full_unstemmed | Study on the biological effects of ZnO nanosheets on EBL cells |
| title_short | Study on the biological effects of ZnO nanosheets on EBL cells |
| title_sort | study on the biological effects of zno nanosheets on ebl cells |
| topic | nanomaterials EBL cells cytotoxicity apoptosis reactive oxygen species |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.915749/full |
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