Changes in Metabolic Profile of Rice Leaves Induced by Humic Acids
The use of humic substances in agriculture as a biostimulant emerged as one of the promising methods to promote sustainable production. Different molecular, biochemical, and physiological processes are triggered, resulting in nutrient efficiency use and protection against abiotic stress. Understandi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/23/3261 |
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| author | Natália Aguiar Canellas Fábio Lopes Olivares Rakiely Martins da Silva Luciano Pasqualoto Canellas |
| author_facet | Natália Aguiar Canellas Fábio Lopes Olivares Rakiely Martins da Silva Luciano Pasqualoto Canellas |
| author_sort | Natália Aguiar Canellas |
| collection | DOAJ |
| description | The use of humic substances in agriculture as a biostimulant emerged as one of the promising methods to promote sustainable production. Different molecular, biochemical, and physiological processes are triggered, resulting in nutrient efficiency use and protection against abiotic stress. Understanding plant changes promoted by humic substances is essential for innovative and tailored biostimulation technologies. Cell metabolites are the final target of the response chain, and the metabolomic approach can be helpful in unveiling pathways related to plant response. This study aimed to evaluate a global metabolic alteration of rice leaves induced by humic acids (HA) applied in a hydroponics system. Using <sup>1</sup>H NMR and GC-TOF/MS analysis, we observed a significant decrease in all main metabolites classes in leaves treated with HA, including lipids, organic acids, amino acids, and carbohydrates. Metabolites in higher concentrations in HA-treated plants are candidates as markers of HA bioactivity, including amino acids, intermediates of tricarboxylic acid cycle, and lipids, and aromatic compounds related to plant-stress response. |
| first_indexed | 2024-03-09T17:35:43Z |
| format | Article |
| id | doaj.art-7bb78bfae7f64a3abc152938e3188e47 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T17:35:43Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-7bb78bfae7f64a3abc152938e3188e472023-11-24T11:54:59ZengMDPI AGPlants2223-77472022-11-011123326110.3390/plants11233261Changes in Metabolic Profile of Rice Leaves Induced by Humic AcidsNatália Aguiar Canellas0Fábio Lopes Olivares1Rakiely Martins da Silva2Luciano Pasqualoto Canellas3Núcleo de Desenvolvimento de Insumos Biológicos para a Agricultura (NUDIBA), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, BrazilNúcleo de Desenvolvimento de Insumos Biológicos para a Agricultura (NUDIBA), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, BrazilNúcleo de Desenvolvimento de Insumos Biológicos para a Agricultura (NUDIBA), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, BrazilNúcleo de Desenvolvimento de Insumos Biológicos para a Agricultura (NUDIBA), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, BrazilThe use of humic substances in agriculture as a biostimulant emerged as one of the promising methods to promote sustainable production. Different molecular, biochemical, and physiological processes are triggered, resulting in nutrient efficiency use and protection against abiotic stress. Understanding plant changes promoted by humic substances is essential for innovative and tailored biostimulation technologies. Cell metabolites are the final target of the response chain, and the metabolomic approach can be helpful in unveiling pathways related to plant response. This study aimed to evaluate a global metabolic alteration of rice leaves induced by humic acids (HA) applied in a hydroponics system. Using <sup>1</sup>H NMR and GC-TOF/MS analysis, we observed a significant decrease in all main metabolites classes in leaves treated with HA, including lipids, organic acids, amino acids, and carbohydrates. Metabolites in higher concentrations in HA-treated plants are candidates as markers of HA bioactivity, including amino acids, intermediates of tricarboxylic acid cycle, and lipids, and aromatic compounds related to plant-stress response.https://www.mdpi.com/2223-7747/11/23/3261metabolomicsprimary and secondary metabolismGC-TOF MSbiofertiliser |
| spellingShingle | Natália Aguiar Canellas Fábio Lopes Olivares Rakiely Martins da Silva Luciano Pasqualoto Canellas Changes in Metabolic Profile of Rice Leaves Induced by Humic Acids Plants metabolomics primary and secondary metabolism GC-TOF MS biofertiliser |
| title | Changes in Metabolic Profile of Rice Leaves Induced by Humic Acids |
| title_full | Changes in Metabolic Profile of Rice Leaves Induced by Humic Acids |
| title_fullStr | Changes in Metabolic Profile of Rice Leaves Induced by Humic Acids |
| title_full_unstemmed | Changes in Metabolic Profile of Rice Leaves Induced by Humic Acids |
| title_short | Changes in Metabolic Profile of Rice Leaves Induced by Humic Acids |
| title_sort | changes in metabolic profile of rice leaves induced by humic acids |
| topic | metabolomics primary and secondary metabolism GC-TOF MS biofertiliser |
| url | https://www.mdpi.com/2223-7747/11/23/3261 |
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