A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol
A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilizati...
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MDPI AG
2020-07-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/7/1346 |
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| author | Mohd Hazani Mat Zaid Jaafar Abdullah Normazida Rozi Aliff Aiman Mohamad Rozlan Sharina Abu Hanifah |
| author_facet | Mohd Hazani Mat Zaid Jaafar Abdullah Normazida Rozi Aliff Aiman Mohamad Rozlan Sharina Abu Hanifah |
| author_sort | Mohd Hazani Mat Zaid |
| collection | DOAJ |
| description | A simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10<sup>−7</sup> to 1.0 × 10 <sup>−12</sup> M, with a detection limit of 0.5 × 10<sup>−12</sup> M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples. |
| first_indexed | 2024-03-10T18:34:37Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T18:34:37Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-7bc0ec5498214a6ea9d793cffb2ffc212023-11-20T06:22:16ZengMDPI AGNanomaterials2079-49912020-07-01107134610.3390/nano10071346A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-EstradiolMohd Hazani Mat Zaid0Jaafar Abdullah1Normazida Rozi2Aliff Aiman Mohamad Rozlan3Sharina Abu Hanifah4Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, MalaysiaDepartment of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaDepartment of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaDepartment of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, MalaysiaA simple and sensitive aptasensor based on conductive carbon nanodots (CDs) was fabricated for the detection of 17ß-Estradiol (E2). In the present study, the hydrothermal synthesis of carbon nanodots was successfully electrodeposited on a screen-printed electrode (SPE) as a platform for immobilization of 76-mer aptamer probe. The morphology and structure of the nanomaterial were characterized by UV-visible absorption spectra, Fluorescence spectra, Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Moreover, cyclic voltammetry and electrochemical impedance spectroscopy were used to investigate the electrochemical performance of the prepared electrodes. Subsequently, impedimetric (EIS) measurements were employed to investigate the relative impedances changes before and after E2 binding, which results in a linear relationship of E2 concentration in the range of 1.0 × 10<sup>−7</sup> to 1.0 × 10 <sup>−12</sup> M, with a detection limit of 0.5 × 10<sup>−12</sup> M. Moreover, the developed biosensor showed high selectivity toward E2 and exhibited excellent discrimination against progesterone (PRG), estriol (E3) and bisphenol A (BPA), respectively. Moreover, the average recovery rate of spiked river water samples with E2 ranged from 98.2% to 103.8%, with relative standard deviations between 1.1% and 3.8%, revealing the potential application of the present biosensor for E2 detection in water samples.https://www.mdpi.com/2079-4991/10/7/1346carbon nanodotsimpedance analysisaptamer17ß-estradiolaptasensor |
| spellingShingle | Mohd Hazani Mat Zaid Jaafar Abdullah Normazida Rozi Aliff Aiman Mohamad Rozlan Sharina Abu Hanifah A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol Nanomaterials carbon nanodots impedance analysis aptamer 17ß-estradiol aptasensor |
| title | A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol |
| title_full | A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol |
| title_fullStr | A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol |
| title_full_unstemmed | A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol |
| title_short | A Sensitive Impedimetric Aptasensor Based on Carbon Nanodots Modified Electrode for Detection of 17ß-Estradiol |
| title_sort | sensitive impedimetric aptasensor based on carbon nanodots modified electrode for detection of 17ss estradiol |
| topic | carbon nanodots impedance analysis aptamer 17ß-estradiol aptasensor |
| url | https://www.mdpi.com/2079-4991/10/7/1346 |
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