Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive Stages
Leaves are essential vegetative organs of plants. Studying the variations in leaf nutrient content and microbial communities of male and female plants at reproductive stages helps us understand allocation and adaptation strategies. This study aimed to determine the nutrient characteristics and micro...
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MDPI AG
2022-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/12/2041 |
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| author | Jian Feng Sohel Rana Zhen Liu Yanmei Wang Qifei Cai Xiaodong Geng Huina Zhou Tao Zhang Shasha Wang Xiaoyan Xue Mingwan Li Razia Sultana Jemim Zhi Li |
| author_facet | Jian Feng Sohel Rana Zhen Liu Yanmei Wang Qifei Cai Xiaodong Geng Huina Zhou Tao Zhang Shasha Wang Xiaoyan Xue Mingwan Li Razia Sultana Jemim Zhi Li |
| author_sort | Jian Feng |
| collection | DOAJ |
| description | Leaves are essential vegetative organs of plants. Studying the variations in leaf nutrient content and microbial communities of male and female plants at reproductive stages helps us understand allocation and adaptation strategies. This study aimed to determine the nutrient characteristics and microbial differences in the leaves of male and female <i>Idesia polycarpa</i> at reproductive stages. Seven-year-old female and male plants were used as test materials in this experiment. The samples were collected at three stages: flowering (May), fruit matter accumulation (July), and fruit ripening (October). The nitrogen (TN), phosphorus (TP), potassium (TK), carbon (TC), and the pH of the female and male leaves were analyzed. In addition, the leaf microbial diversity and differential metabolites were determined using the Illumina high-throughput sequencing method and the ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method at the reproductive developmental stages. This study found that male and female plant leaves had different TN and TK contents over time but no difference in TC and TP content. The significant differences in bacterial diversity between male and female plants and the richness of the fungi of male plants at the flowering and fruit maturity stages were observed. Proteobacteria, Pseudomonadaceae, Ascomycota, and Aspergillus were the dominant bacteria and fungi in the <i>Idesia polycarpa</i> leaves. The presence of microorganisms differed in the two sexes in different periods. Alphaproteobacteria and Sordariomycetes were the indicator groups for male leaves, and Pseudomonas and Sordariomycetes were the indicator groups for female leaves. Significant differences in phenolic acid were found between male and female leaves. A KEGG enrichment analysis revealed that differential metabolites were enriched in metabolic pathways, amino acid biosynthesis, and the nucleotide metabolism. According to a correlation analysis, leaf TK and TP were strongly correlated with endophytic bacteria abundance and differential metabolite composition. This study revealed the changes in substances and microorganisms in the leaves of male and female plants in their reproductive stages. It provides a theoretical basis for developing and utilizing the leaves of <i>Idesia polycarpa</i> and for field management. |
| first_indexed | 2024-03-09T16:11:04Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:11:04Z |
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| spelling | doaj.art-7bc16c3352474f86a02aeb2817d1dc502023-11-24T16:12:33ZengMDPI AGLife2075-17292022-12-011212204110.3390/life12122041Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive StagesJian Feng0Sohel Rana1Zhen Liu2Yanmei Wang3Qifei Cai4Xiaodong Geng5Huina Zhou6Tao Zhang7Shasha Wang8Xiaoyan Xue9Mingwan Li10Razia Sultana Jemim11Zhi Li12College of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaLeaves are essential vegetative organs of plants. Studying the variations in leaf nutrient content and microbial communities of male and female plants at reproductive stages helps us understand allocation and adaptation strategies. This study aimed to determine the nutrient characteristics and microbial differences in the leaves of male and female <i>Idesia polycarpa</i> at reproductive stages. Seven-year-old female and male plants were used as test materials in this experiment. The samples were collected at three stages: flowering (May), fruit matter accumulation (July), and fruit ripening (October). The nitrogen (TN), phosphorus (TP), potassium (TK), carbon (TC), and the pH of the female and male leaves were analyzed. In addition, the leaf microbial diversity and differential metabolites were determined using the Illumina high-throughput sequencing method and the ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method at the reproductive developmental stages. This study found that male and female plant leaves had different TN and TK contents over time but no difference in TC and TP content. The significant differences in bacterial diversity between male and female plants and the richness of the fungi of male plants at the flowering and fruit maturity stages were observed. Proteobacteria, Pseudomonadaceae, Ascomycota, and Aspergillus were the dominant bacteria and fungi in the <i>Idesia polycarpa</i> leaves. The presence of microorganisms differed in the two sexes in different periods. Alphaproteobacteria and Sordariomycetes were the indicator groups for male leaves, and Pseudomonas and Sordariomycetes were the indicator groups for female leaves. Significant differences in phenolic acid were found between male and female leaves. A KEGG enrichment analysis revealed that differential metabolites were enriched in metabolic pathways, amino acid biosynthesis, and the nucleotide metabolism. According to a correlation analysis, leaf TK and TP were strongly correlated with endophytic bacteria abundance and differential metabolite composition. This study revealed the changes in substances and microorganisms in the leaves of male and female plants in their reproductive stages. It provides a theoretical basis for developing and utilizing the leaves of <i>Idesia polycarpa</i> and for field management.https://www.mdpi.com/2075-1729/12/12/2041dioecious plantreproductive stagesnutrient characteristicsendophytesmetabolite |
| spellingShingle | Jian Feng Sohel Rana Zhen Liu Yanmei Wang Qifei Cai Xiaodong Geng Huina Zhou Tao Zhang Shasha Wang Xiaoyan Xue Mingwan Li Razia Sultana Jemim Zhi Li Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive Stages Life dioecious plant reproductive stages nutrient characteristics endophytes metabolite |
| title | Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive Stages |
| title_full | Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive Stages |
| title_fullStr | Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive Stages |
| title_full_unstemmed | Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive Stages |
| title_short | Diversity Analysis of Leaf Nutrient Endophytes and Metabolites in Dioecious <i>Idesia polycarpa</i> Maxim Leaves during Reproductive Stages |
| title_sort | diversity analysis of leaf nutrient endophytes and metabolites in dioecious i idesia polycarpa i maxim leaves during reproductive stages |
| topic | dioecious plant reproductive stages nutrient characteristics endophytes metabolite |
| url | https://www.mdpi.com/2075-1729/12/12/2041 |
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