Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile
Currently, inflammatory bowel disease (IBD) is a serious public health problem on the rise worldwide. In this work, we utilized the zebrafish to introduce a new model of intestinal inflammation triggered by food intake. Taking advantage of the translucency of the larvae and the availability of trans...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-04-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00610/full |
| _version_ | 1811281357170540544 |
|---|---|
| author | Maximo Coronado Camila J. Solis Camila J. Solis Pedro P. Hernandez Carmen G. Feijóo Carmen G. Feijóo |
| author_facet | Maximo Coronado Camila J. Solis Camila J. Solis Pedro P. Hernandez Carmen G. Feijóo Carmen G. Feijóo |
| author_sort | Maximo Coronado |
| collection | DOAJ |
| description | Currently, inflammatory bowel disease (IBD) is a serious public health problem on the rise worldwide. In this work, we utilized the zebrafish to introduce a new model of intestinal inflammation triggered by food intake. Taking advantage of the translucency of the larvae and the availability of transgenic zebrafish lines with fluorescently labeled macrophages, neutrophils, or lymphocytes, we studied the behavior of these cell types in vivo during the course of inflammation. We established two feeding strategies, the first using fish that were not previously exposed to food (naïve strategy) and the second in which fish were initially exposed to normal food (developed strategy). In both strategies, we analyzed the effect of subsequent intake of a control or a soybean meal diet. Our results showed increased numbers of innate immune cells in the gut in both the naïve or developed protocols. Likewise, macrophages underwent drastic morphological changes after feeding, switching from a small and rounded contour to a larger and dendritic shape. Lymphocytes colonized the intestine as early as 5 days post fertilization and increased in numbers during the inflammatory process. Gene expression analysis indicated that lymphocytes present in the intestine correspond to T helper cells. Interestingly, control diet only induced a regulatory T cell profile in the developed model. On the contrary, soybean meal diet induced a Th17 response both in naïve and developed model. In addition, when feeding was performed in rag1-deficient fish, intestinal inflammation was not induced indicating that inflammation induced by soybean meal is T cell-dependent. |
| first_indexed | 2024-04-13T01:30:56Z |
| format | Article |
| id | doaj.art-7bc2574fce0f429d966992501e63cc0a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T01:30:56Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7bc2574fce0f429d966992501e63cc0a2022-12-22T03:08:30ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-04-011010.3389/fimmu.2019.00610409146Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine ProfileMaximo Coronado0Camila J. Solis1Camila J. Solis2Pedro P. Hernandez3Carmen G. Feijóo4Carmen G. Feijóo5Departamento de Ciencias Biologicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, ChileMillennium Nucleus in the Biology of Intestinal Microbiota, Santiago, ChileEscuela de Tecnología Médica, Facultad de Ciencias de la Salud, Universidad San Sebastian, Santiago, ChileMacrophages and Development of Immunity, Institute Pasteur, Paris, FranceDepartamento de Ciencias Biologicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, ChileMillennium Nucleus in the Biology of Intestinal Microbiota, Santiago, ChileCurrently, inflammatory bowel disease (IBD) is a serious public health problem on the rise worldwide. In this work, we utilized the zebrafish to introduce a new model of intestinal inflammation triggered by food intake. Taking advantage of the translucency of the larvae and the availability of transgenic zebrafish lines with fluorescently labeled macrophages, neutrophils, or lymphocytes, we studied the behavior of these cell types in vivo during the course of inflammation. We established two feeding strategies, the first using fish that were not previously exposed to food (naïve strategy) and the second in which fish were initially exposed to normal food (developed strategy). In both strategies, we analyzed the effect of subsequent intake of a control or a soybean meal diet. Our results showed increased numbers of innate immune cells in the gut in both the naïve or developed protocols. Likewise, macrophages underwent drastic morphological changes after feeding, switching from a small and rounded contour to a larger and dendritic shape. Lymphocytes colonized the intestine as early as 5 days post fertilization and increased in numbers during the inflammatory process. Gene expression analysis indicated that lymphocytes present in the intestine correspond to T helper cells. Interestingly, control diet only induced a regulatory T cell profile in the developed model. On the contrary, soybean meal diet induced a Th17 response both in naïve and developed model. In addition, when feeding was performed in rag1-deficient fish, intestinal inflammation was not induced indicating that inflammation induced by soybean meal is T cell-dependent.https://www.frontiersin.org/article/10.3389/fimmu.2019.00610/fullintestinal inflammationzebrafishinnate immuneadaptive immunityTh17 T cellsmacrophage |
| spellingShingle | Maximo Coronado Camila J. Solis Camila J. Solis Pedro P. Hernandez Carmen G. Feijóo Carmen G. Feijóo Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile Frontiers in Immunology intestinal inflammation zebrafish innate immune adaptive immunity Th17 T cells macrophage |
| title | Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile |
| title_full | Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile |
| title_fullStr | Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile |
| title_full_unstemmed | Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile |
| title_short | Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile |
| title_sort | soybean meal induced intestinal inflammation in zebrafish is t cell dependent and has a th17 cytokine profile |
| topic | intestinal inflammation zebrafish innate immune adaptive immunity Th17 T cells macrophage |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00610/full |
| work_keys_str_mv | AT maximocoronado soybeanmealinducedintestinalinflammationinzebrafishistcelldependentandhasath17cytokineprofile AT camilajsolis soybeanmealinducedintestinalinflammationinzebrafishistcelldependentandhasath17cytokineprofile AT camilajsolis soybeanmealinducedintestinalinflammationinzebrafishistcelldependentandhasath17cytokineprofile AT pedrophernandez soybeanmealinducedintestinalinflammationinzebrafishistcelldependentandhasath17cytokineprofile AT carmengfeijoo soybeanmealinducedintestinalinflammationinzebrafishistcelldependentandhasath17cytokineprofile AT carmengfeijoo soybeanmealinducedintestinalinflammationinzebrafishistcelldependentandhasath17cytokineprofile |