A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome
Acute respiratory distress syndrome (ARDS) is a diffuse, acute, inflammatory lung disease characterized by a severe respiratory failure. Recognizing and promptly treating ARDS is critical to combat the high mortality associated with the disease. Despite a significant progress in the treatment of ARD...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2021.640042/full |
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| author | Gilles Parzibut Monique Henket Catherine Moermans Ingrid Struman Edouard Louis Edouard Louis Michel Malaise Michel Malaise Renaud Louis Renaud Louis Benoît Misset Makon-Sébastien Njock Makon-Sébastien Njock Makon-Sébastien Njock Makon-Sébastien Njock Julien Guiot Julien Guiot Julien Guiot |
| author_facet | Gilles Parzibut Monique Henket Catherine Moermans Ingrid Struman Edouard Louis Edouard Louis Michel Malaise Michel Malaise Renaud Louis Renaud Louis Benoît Misset Makon-Sébastien Njock Makon-Sébastien Njock Makon-Sébastien Njock Makon-Sébastien Njock Julien Guiot Julien Guiot Julien Guiot |
| author_sort | Gilles Parzibut |
| collection | DOAJ |
| description | Acute respiratory distress syndrome (ARDS) is a diffuse, acute, inflammatory lung disease characterized by a severe respiratory failure. Recognizing and promptly treating ARDS is critical to combat the high mortality associated with the disease. Despite a significant progress in the treatment of ARDS, our ability to identify early patients and predict outcomes remains limited. The development of novel biomarkers is crucial. In this study, we profiled microRNA (miRNA) expression of plasma-derived exosomes in ARDS disease by small RNA sequencing. Sequencing of 8 ARDS patients and 10 healthy subjects (HSs) allowed to identify 12 differentially expressed exosomal miRNAs (adjusted p < 0.05). Pathway analysis of their predicted targets revealed enrichment in several biological processes in agreement with ARDS pathophysiology, such as inflammation, immune cell activation, and fibrosis. By quantitative RT-PCR, we validated the alteration of nine exosomal miRNAs in an independent cohort of 15 ARDS patients and 20 HSs, among which seven present high capability in discriminating ARDS patients from HSs (area under the curve > 0.8) (miR-130a-3p, miR-221-3p, miR-24-3p, miR-98-3p, Let-7d-3p, miR-1273a, and miR-193a-5p). These findings highlight exosomal miRNA dysregulation in the plasma of ARDS patients which provide promising diagnostic biomarkers and open new perspectives for the development of therapeutics. |
| first_indexed | 2024-12-16T18:55:44Z |
| format | Article |
| id | doaj.art-7bc6b74baae542cdb8aa7d68579b7dfa |
| institution | Directory Open Access Journal |
| issn | 2296-889X |
| language | English |
| last_indexed | 2024-12-16T18:55:44Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-7bc6b74baae542cdb8aa7d68579b7dfa2022-12-21T22:20:32ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2021-07-01810.3389/fmolb.2021.640042640042A Blood Exosomal miRNA Signature in Acute Respiratory Distress SyndromeGilles Parzibut0Monique Henket1Catherine Moermans2Ingrid Struman3Edouard Louis4Edouard Louis5Michel Malaise6Michel Malaise7Renaud Louis8Renaud Louis9Benoît Misset10Makon-Sébastien Njock11Makon-Sébastien Njock12Makon-Sébastien Njock13Makon-Sébastien Njock14Julien Guiot15Julien Guiot16Julien Guiot17Department of Intensive Care, University Hospital of Liège, Liège, BelgiumLaboratory of Pneumology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumLaboratory of Pneumology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumLaboratory of Molecular Angiogenesis, GIGA Research Center, University of Liège, Liège, BelgiumLaboratory of Gastroenterology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumFibropole Research Group, University Hospital of Liège, Liège, BelgiumFibropole Research Group, University Hospital of Liège, Liège, BelgiumLaboratory of Rheumatology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumLaboratory of Pneumology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumFibropole Research Group, University Hospital of Liège, Liège, BelgiumDepartment of Intensive Care, University Hospital of Liège, Liège, BelgiumLaboratory of Pneumology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumLaboratory of Gastroenterology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumFibropole Research Group, University Hospital of Liège, Liège, BelgiumLaboratory of Rheumatology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumDepartment of Intensive Care, University Hospital of Liège, Liège, BelgiumLaboratory of Pneumology, GIGA Research Center, University of Liège, University Hospital of Liège, Liège, BelgiumFibropole Research Group, University Hospital of Liège, Liège, BelgiumAcute respiratory distress syndrome (ARDS) is a diffuse, acute, inflammatory lung disease characterized by a severe respiratory failure. Recognizing and promptly treating ARDS is critical to combat the high mortality associated with the disease. Despite a significant progress in the treatment of ARDS, our ability to identify early patients and predict outcomes remains limited. The development of novel biomarkers is crucial. In this study, we profiled microRNA (miRNA) expression of plasma-derived exosomes in ARDS disease by small RNA sequencing. Sequencing of 8 ARDS patients and 10 healthy subjects (HSs) allowed to identify 12 differentially expressed exosomal miRNAs (adjusted p < 0.05). Pathway analysis of their predicted targets revealed enrichment in several biological processes in agreement with ARDS pathophysiology, such as inflammation, immune cell activation, and fibrosis. By quantitative RT-PCR, we validated the alteration of nine exosomal miRNAs in an independent cohort of 15 ARDS patients and 20 HSs, among which seven present high capability in discriminating ARDS patients from HSs (area under the curve > 0.8) (miR-130a-3p, miR-221-3p, miR-24-3p, miR-98-3p, Let-7d-3p, miR-1273a, and miR-193a-5p). These findings highlight exosomal miRNA dysregulation in the plasma of ARDS patients which provide promising diagnostic biomarkers and open new perspectives for the development of therapeutics.https://www.frontiersin.org/articles/10.3389/fmolb.2021.640042/fullacute respiratory distress syndromeacute lung injurydiagnostic biomarkersexosomesmicroRNAsinflammation |
| spellingShingle | Gilles Parzibut Monique Henket Catherine Moermans Ingrid Struman Edouard Louis Edouard Louis Michel Malaise Michel Malaise Renaud Louis Renaud Louis Benoît Misset Makon-Sébastien Njock Makon-Sébastien Njock Makon-Sébastien Njock Makon-Sébastien Njock Julien Guiot Julien Guiot Julien Guiot A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome Frontiers in Molecular Biosciences acute respiratory distress syndrome acute lung injury diagnostic biomarkers exosomes microRNAs inflammation |
| title | A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome |
| title_full | A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome |
| title_fullStr | A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome |
| title_full_unstemmed | A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome |
| title_short | A Blood Exosomal miRNA Signature in Acute Respiratory Distress Syndrome |
| title_sort | blood exosomal mirna signature in acute respiratory distress syndrome |
| topic | acute respiratory distress syndrome acute lung injury diagnostic biomarkers exosomes microRNAs inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2021.640042/full |
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