Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function
Abstract Background MicroRNAs are paramount in post transcriptional gene regulation. We investigated platelet miRNAs in patients with CAD and examined potential associations with course of left ventricular ejection fraction (LVEF%). Materials and methods In a first cohort, 62 MiRNAs were measured in...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Cardiovascular Disorders |
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| Online Access: | https://doi.org/10.1186/s12872-023-03362-0 |
| _version_ | 1797779060717256704 |
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| author | Andreas Goldschmied Bernhard Drotleff Stefan Winter Elke Schaeffeler Matthias Schwab Meinrad Gawaz Tobias Geisler Dominik Rath |
| author_facet | Andreas Goldschmied Bernhard Drotleff Stefan Winter Elke Schaeffeler Matthias Schwab Meinrad Gawaz Tobias Geisler Dominik Rath |
| author_sort | Andreas Goldschmied |
| collection | DOAJ |
| description | Abstract Background MicroRNAs are paramount in post transcriptional gene regulation. We investigated platelet miRNAs in patients with CAD and examined potential associations with course of left ventricular ejection fraction (LVEF%). Materials and methods In a first cohort, 62 MiRNAs were measured in platelets of 100 patients suffering from CAD. Expression profiles of individuals with chronic coronary syndrome (CCS) and MI were compared (CCS n = 67, MI n = 33). Also, associations between miRNA profiles and change in left ventricular ejection fraction (LVEF%) were investigated. In a second cohort of patients suffering from CCS (n = 10), MI (n = 11) or no CAD (n = 13), we measured miRNA expression in platelets, platelet supernatant and serum. This was carried out before and after in vitro platelet activation with CRP. Results Platelet miRNAs 103a-3p and 155-5p demonstrated higher expression in patients with CCS then in individuals with MI. Furthermore, multiple miRNAs were significantly higher expressed in matched controls compared to MI patients. 8 miRNAs showed higher expression in patients with improving LVEF% after a 1-year follow-up. In our second cohort, we found higher concentrations of 6 miRNAs in the platelet supernatant of patients with CCS, MI and no CAD after in vitro platelet activation. Most of these miRNAs showed a higher abundance in serum of MI patients as compared to CCS. Conclusion Several miRNAs show higher expression in platelets of CCS compared to MI. After in vitro platelet activation, a release of multiple miRNAs out of the thrombocyte was observed. Furthermore, upregulation of serum miRNAs was found in MI patients when compared to CCS patients and individuals without CAD. Hence, platelets could present a source of upregulated circulating miRNAs in MI and additionally affect course of LVEF%. |
| first_indexed | 2024-03-12T23:26:16Z |
| format | Article |
| id | doaj.art-7bceb6f62cc34f639826b5c1ed7910e1 |
| institution | Directory Open Access Journal |
| issn | 1471-2261 |
| language | English |
| last_indexed | 2024-03-12T23:26:16Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-7bceb6f62cc34f639826b5c1ed7910e12023-07-16T11:08:59ZengBMCBMC Cardiovascular Disorders1471-22612023-07-0123111110.1186/s12872-023-03362-0Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic functionAndreas Goldschmied0Bernhard Drotleff1Stefan Winter2Elke Schaeffeler3Matthias Schwab4Meinrad Gawaz5Tobias Geisler6Dominik Rath7Department of Cardiology, University Hospital TübingenEuropean Molecular Biology LaboratoryUniversity of TübingenUniversity of TübingenDr. Margarete Fischer-Bosch-Institute of Clinical PharmacologyDepartment of Cardiology, University Hospital TübingenDepartment of Cardiology, University Hospital TübingenDepartment of Cardiology, University Hospital TübingenAbstract Background MicroRNAs are paramount in post transcriptional gene regulation. We investigated platelet miRNAs in patients with CAD and examined potential associations with course of left ventricular ejection fraction (LVEF%). Materials and methods In a first cohort, 62 MiRNAs were measured in platelets of 100 patients suffering from CAD. Expression profiles of individuals with chronic coronary syndrome (CCS) and MI were compared (CCS n = 67, MI n = 33). Also, associations between miRNA profiles and change in left ventricular ejection fraction (LVEF%) were investigated. In a second cohort of patients suffering from CCS (n = 10), MI (n = 11) or no CAD (n = 13), we measured miRNA expression in platelets, platelet supernatant and serum. This was carried out before and after in vitro platelet activation with CRP. Results Platelet miRNAs 103a-3p and 155-5p demonstrated higher expression in patients with CCS then in individuals with MI. Furthermore, multiple miRNAs were significantly higher expressed in matched controls compared to MI patients. 8 miRNAs showed higher expression in patients with improving LVEF% after a 1-year follow-up. In our second cohort, we found higher concentrations of 6 miRNAs in the platelet supernatant of patients with CCS, MI and no CAD after in vitro platelet activation. Most of these miRNAs showed a higher abundance in serum of MI patients as compared to CCS. Conclusion Several miRNAs show higher expression in platelets of CCS compared to MI. After in vitro platelet activation, a release of multiple miRNAs out of the thrombocyte was observed. Furthermore, upregulation of serum miRNAs was found in MI patients when compared to CCS patients and individuals without CAD. Hence, platelets could present a source of upregulated circulating miRNAs in MI and additionally affect course of LVEF%.https://doi.org/10.1186/s12872-023-03362-0Platelet microRNACoronary artery diseaseMyocardial infarctionMiRNA 103a-3pMiRNA 155-5pLeft ventricular ejection fraction |
| spellingShingle | Andreas Goldschmied Bernhard Drotleff Stefan Winter Elke Schaeffeler Matthias Schwab Meinrad Gawaz Tobias Geisler Dominik Rath Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function BMC Cardiovascular Disorders Platelet microRNA Coronary artery disease Myocardial infarction MiRNA 103a-3p MiRNA 155-5p Left ventricular ejection fraction |
| title | Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function |
| title_full | Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function |
| title_fullStr | Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function |
| title_full_unstemmed | Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function |
| title_short | Platelet miRNAs: differential expression in coronary artery disease and associations with course of left ventricular systolic function |
| title_sort | platelet mirnas differential expression in coronary artery disease and associations with course of left ventricular systolic function |
| topic | Platelet microRNA Coronary artery disease Myocardial infarction MiRNA 103a-3p MiRNA 155-5p Left ventricular ejection fraction |
| url | https://doi.org/10.1186/s12872-023-03362-0 |
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