Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase
Summary: The spindle assembly checkpoint (SAC) monitors chromosome attachment defects, and the assembly of SAC proteins at kinetochores is essential for its activation, but the SAC disassembly process remains unknown. We found that deletion of a 14-3-3 protein, Bmh1, or hyperactivation of Cdc14 earl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-02-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124716000279 |
| _version_ | 1818992379246411776 |
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| author | Michael Bokros Curtis Gravenmier Fengzhi Jin Daniel Richmond Yanchang Wang |
| author_facet | Michael Bokros Curtis Gravenmier Fengzhi Jin Daniel Richmond Yanchang Wang |
| author_sort | Michael Bokros |
| collection | DOAJ |
| description | Summary: The spindle assembly checkpoint (SAC) monitors chromosome attachment defects, and the assembly of SAC proteins at kinetochores is essential for its activation, but the SAC disassembly process remains unknown. We found that deletion of a 14-3-3 protein, Bmh1, or hyperactivation of Cdc14 early anaphase release (FEAR) allows premature SAC silencing in budding yeast, which depends on a kinetochore protein Fin1 that forms a complex with protein phosphatase PP1. Previous works suggest that FEAR-dependent Fin1 dephosphorylation promotes Bmh1-Fin1 dissociation, which enables kinetochore recruitment of Fin1-PP1. We found persistent kinetochore association of SAC protein Bub1 in fin1Δ mutants after anaphase entry. Therefore, we revealed a mechanism that clears SAC proteins from kinetochores. After anaphase entry, FEAR activation promotes kinetochore enrichment of Fin1-PP1, resulting in SAC disassembly at kinetochores. This mechanism is required for efficient SAC silencing after SAC is challenged, and untimely Fin1-kinetochore association causes premature SAC silencing and chromosome missegregation. : Bokros et al. examine the process of spindle assembly checkpoint (SAC) disassembly and find that the kinetochore protein Fin1, in complex with the protein phosphatase PP1, localizes to the kinetochores during anaphase. Fin1-PP1 promotes the dissociation of the SAC protein Bub1 from kinetochores, leading to SAC disassembly. Keywords: spindle assembly checkpoint, Bmh1, Fin1, PP1, FEAR pathway |
| first_indexed | 2024-12-20T20:25:13Z |
| format | Article |
| id | doaj.art-7bcfa525b88e447c856a07b286dd5576 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-20T20:25:13Z |
| publishDate | 2016-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-7bcfa525b88e447c856a07b286dd55762022-12-21T19:27:29ZengElsevierCell Reports2211-12472016-02-0114510741085Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in AnaphaseMichael Bokros0Curtis Gravenmier1Fengzhi Jin2Daniel Richmond3Yanchang Wang4Department of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USADepartment of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306-4300, USADepartment of Biomedical Sciences, College of Medicine, Florida State University, 1115 West Call Street, Tallahassee, FL 32306-4300, USA; Corresponding authorSummary: The spindle assembly checkpoint (SAC) monitors chromosome attachment defects, and the assembly of SAC proteins at kinetochores is essential for its activation, but the SAC disassembly process remains unknown. We found that deletion of a 14-3-3 protein, Bmh1, or hyperactivation of Cdc14 early anaphase release (FEAR) allows premature SAC silencing in budding yeast, which depends on a kinetochore protein Fin1 that forms a complex with protein phosphatase PP1. Previous works suggest that FEAR-dependent Fin1 dephosphorylation promotes Bmh1-Fin1 dissociation, which enables kinetochore recruitment of Fin1-PP1. We found persistent kinetochore association of SAC protein Bub1 in fin1Δ mutants after anaphase entry. Therefore, we revealed a mechanism that clears SAC proteins from kinetochores. After anaphase entry, FEAR activation promotes kinetochore enrichment of Fin1-PP1, resulting in SAC disassembly at kinetochores. This mechanism is required for efficient SAC silencing after SAC is challenged, and untimely Fin1-kinetochore association causes premature SAC silencing and chromosome missegregation. : Bokros et al. examine the process of spindle assembly checkpoint (SAC) disassembly and find that the kinetochore protein Fin1, in complex with the protein phosphatase PP1, localizes to the kinetochores during anaphase. Fin1-PP1 promotes the dissociation of the SAC protein Bub1 from kinetochores, leading to SAC disassembly. Keywords: spindle assembly checkpoint, Bmh1, Fin1, PP1, FEAR pathwayhttp://www.sciencedirect.com/science/article/pii/S2211124716000279 |
| spellingShingle | Michael Bokros Curtis Gravenmier Fengzhi Jin Daniel Richmond Yanchang Wang Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase Cell Reports |
| title | Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase |
| title_full | Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase |
| title_fullStr | Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase |
| title_full_unstemmed | Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase |
| title_short | Fin1-PP1 Helps Clear Spindle Assembly Checkpoint Protein Bub1 from Kinetochores in Anaphase |
| title_sort | fin1 pp1 helps clear spindle assembly checkpoint protein bub1 from kinetochores in anaphase |
| url | http://www.sciencedirect.com/science/article/pii/S2211124716000279 |
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