Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activation
Objective(s):HTLV-1 is the first human retrovirus that has been recognized and is associated with HAM/TSP and ATLL. Studies have shown that less than five percent of HTLV-1 infected carriers develop HAM/TSP or ATLL and about ninety-five percent remain asymptomatic. Therefore, the proviral load with...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2014-07-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_3033_c4ddba505aa4b0b02e9884929a7d4c49.html |
| _version_ | 1811258180033839104 |
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| author | Atefeh Yari Seyyed Abdolrahim Rezaee Narges Valizadeh Taraneh Rajaee Seyyed Mohammad Jazayeri Mojdeh Soltani Mehdi Norouzi |
| author_facet | Atefeh Yari Seyyed Abdolrahim Rezaee Narges Valizadeh Taraneh Rajaee Seyyed Mohammad Jazayeri Mojdeh Soltani Mehdi Norouzi |
| author_sort | Atefeh Yari |
| collection | DOAJ |
| description | Objective(s):HTLV-1 is the first human retrovirus that has been recognized and is associated with HAM/TSP and ATLL. Studies have shown that less than five percent of HTLV-1 infected carriers develop HAM/TSP or ATLL and about ninety-five percent remain asymptomatic. Therefore, the proviral load with Tax may affect cellular genes such as cytokines and oncogenes, as well as involve in pathogenicity.
Materials and Methods:Thirty HAM/TSP patients, thirty HTLV-1 healthy carriers, and MT-2 cell line were evaluated for HTLV-1 activity. PBMCs were isolated and activated using PMA and ionomycine. Real-time PCR and TaqMan methods were performed using specific primers and fluorescence probes for Tax expression and proviral load assessment. Β2microglobulin (β2m) and albumin were used as controls in Tax expression and in proviral load, respectively.
Results: An insignificant increase in Tax expression was observed in rest PBMCs of HAM/TSP patients compared to healthy carriers. However, after lymphocyte activation there was a significant increase in Tax expression in HAM/TSP patients (P=0.042). The Proviral load in patients was significantly higher than in carriers. Moreover, there was a significant correlation between Tax mRNA expression in activated PBMCs and proviral load (R=0.37, P=0.012).
Conclusion: Although proviral load had been addressed as a valuable index for monitoring HTLV-1 infected subjects, the results of this study demonstrated that Tax expression in activated PBMCs along with proviral load assessment in HAM/TSP patients are a more reliable factor for determining the prognosis and monitoring healthy carriers and HAM/TSP patients. |
| first_indexed | 2024-04-12T18:09:14Z |
| format | Article |
| id | doaj.art-7bd01fb713f1440eb1cb3f8d404208d1 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-12T18:09:14Z |
| publishDate | 2014-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-7bd01fb713f1440eb1cb3f8d404208d12022-12-22T03:21:53ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742014-07-011775315363033Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activationAtefeh Yari0Seyyed Abdolrahim Rezaee1Narges Valizadeh2Taraneh Rajaee3Seyyed Mohammad Jazayeri4Mojdeh Soltani5Mehdi Norouzi6Microbiology and Virology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran Virology Department, Public Health School, Tehran University of Medical Sciences, Tehran, IranImmunology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranInflammation and Inflammatory diseases Research Center, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, IranInflammation and Inflammatory diseases Research Center, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, IranVirology Department, Public Health School, Tehran University of Medical Sciences, Tehran, IranVirology Department, Public Health School, Tehran University of Medical Sciences, Tehran, IranVirology Department, Public Health School, Tehran University of Medical Sciences, Tehran, IranObjective(s):HTLV-1 is the first human retrovirus that has been recognized and is associated with HAM/TSP and ATLL. Studies have shown that less than five percent of HTLV-1 infected carriers develop HAM/TSP or ATLL and about ninety-five percent remain asymptomatic. Therefore, the proviral load with Tax may affect cellular genes such as cytokines and oncogenes, as well as involve in pathogenicity. Materials and Methods:Thirty HAM/TSP patients, thirty HTLV-1 healthy carriers, and MT-2 cell line were evaluated for HTLV-1 activity. PBMCs were isolated and activated using PMA and ionomycine. Real-time PCR and TaqMan methods were performed using specific primers and fluorescence probes for Tax expression and proviral load assessment. Β2microglobulin (β2m) and albumin were used as controls in Tax expression and in proviral load, respectively. Results: An insignificant increase in Tax expression was observed in rest PBMCs of HAM/TSP patients compared to healthy carriers. However, after lymphocyte activation there was a significant increase in Tax expression in HAM/TSP patients (P=0.042). The Proviral load in patients was significantly higher than in carriers. Moreover, there was a significant correlation between Tax mRNA expression in activated PBMCs and proviral load (R=0.37, P=0.012). Conclusion: Although proviral load had been addressed as a valuable index for monitoring HTLV-1 infected subjects, the results of this study demonstrated that Tax expression in activated PBMCs along with proviral load assessment in HAM/TSP patients are a more reliable factor for determining the prognosis and monitoring healthy carriers and HAM/TSP patients.http://ijbms.mums.ac.ir/pdf_3033_c4ddba505aa4b0b02e9884929a7d4c49.htmlGene expressionHAM/TSPHTLV-1Proviral loadTax mRNA |
| spellingShingle | Atefeh Yari Seyyed Abdolrahim Rezaee Narges Valizadeh Taraneh Rajaee Seyyed Mohammad Jazayeri Mojdeh Soltani Mehdi Norouzi Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activation Iranian Journal of Basic Medical Sciences Gene expression HAM/TSP HTLV-1 Proviral load Tax mRNA |
| title | Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activation |
| title_full | Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activation |
| title_fullStr | Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activation |
| title_full_unstemmed | Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activation |
| title_short | Evaluation of HTLV-1 activity in HAM/TSP patients using proviral load and Tax mRNA expression after In Vitro lymphocyte activation |
| title_sort | evaluation of htlv 1 activity in ham tsp patients using proviral load and tax mrna expression after in vitro lymphocyte activation |
| topic | Gene expression HAM/TSP HTLV-1 Proviral load Tax mRNA |
| url | http://ijbms.mums.ac.ir/pdf_3033_c4ddba505aa4b0b02e9884929a7d4c49.html |
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