In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract
Dysphania ambrosioides (L.) holds a prominent place in Moroccan folk medicine due to its therapeutic attributes. Despite its widespread use, instances of inadvertent intoxication linked to its consumption have been reported. This study aims to evaluate the potential cytogenotoxic effects of D. ambro...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2024-03-01
|
| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2023-0207 |
| _version_ | 1797266220258426880 |
|---|---|
| author | El-Bouzidi Laila khadra Ahmed Zefzoufi Manal Sissi Saida El-Abbassi Abdelilah Bekkouche Khalid Sellami Souad Rais Hanane |
| author_facet | El-Bouzidi Laila khadra Ahmed Zefzoufi Manal Sissi Saida El-Abbassi Abdelilah Bekkouche Khalid Sellami Souad Rais Hanane |
| author_sort | El-Bouzidi Laila |
| collection | DOAJ |
| description | Dysphania ambrosioides (L.) holds a prominent place in Moroccan folk medicine due to its therapeutic attributes. Despite its widespread use, instances of inadvertent intoxication linked to its consumption have been reported. This study aims to evaluate the potential cytogenotoxic effects of D. ambrosioides leaf aqueous extract (DAAE) and explore the prospective protective role of vitamin C (l-ascorbic acid) through the micronucleus test conducted on (1) Vicia faba root-tip meristem and (2) mouse bone marrow cells. In addition, antioxidant enzyme activities, specifically superoxide dismutase (SOD) and catalase (CAT), were evaluated in V. faba treated with DAAE. After a 7-day daily administration of DAAE to mice, serum biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine, and uric acid) were measured, and histological examination of liver and kidney tissues was performed. The results indicated that DAAE had dose-dependent cytotoxic and genotoxic effects on both biological models. Furthermore, V. faba treated with DAAE showed significant increases in the activities of SOD and CAT enzymes. Mice treated with DAAE exhibited significant elevations in serum biochemical parameters compared to the control group. Histological examination of liver and kidney tissues revealed hepatic degeneration, glomerular shrinkage, and distinct vacuolated tubular epithelial cells. The cotreatment with vitamin C demonstrated a significant protective effect against DAAE-induced cytogenotoxicity. These findings underscore the importance of vitamin C as a protective agent against oxidative stress and cytogenotoxicity induced by DAAE and recommend its use in any DAAE-based preparation. |
| first_indexed | 2024-04-25T00:57:13Z |
| format | Article |
| id | doaj.art-7bd4911b48c34d10aefdeb54c483a5ef |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-04-25T00:57:13Z |
| publishDate | 2024-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-7bd4911b48c34d10aefdeb54c483a5ef2024-03-11T10:04:00ZengDe GruyterOpen Chemistry2391-54202024-03-012212182710.1515/chem-2023-0207In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extractEl-Bouzidi Laila0khadra Ahmed1Zefzoufi Manal2Sissi Saida3El-Abbassi Abdelilah4Bekkouche Khalid5Sellami Souad6Rais Hanane7Laboratory of Agri-Food, Biotechnologies and Valorization of Plant Bioresources (AGROBIOVAL). Agro Biotech Center, Labeled Research Unit-CNRST N°5, Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000, Marrakech, MoroccoLaboratory of Agri-Food, Biotechnologies and Valorization of Plant Bioresources (AGROBIOVAL). Agro Biotech Center, Labeled Research Unit-CNRST N°5, Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000, Marrakech, MoroccoLaboratory of Sustainable Development and Health Research, Faculty of Sciences and Techniques, Cadi Ayyad University, Abdelkarim AlKhattabi Avenue, PO. Box: 549, Marrakech, 40000, MoroccoLaboratory of Agri-Food, Biotechnologies and Valorization of Plant Bioresources (AGROBIOVAL). Agro Biotech Center, Labeled Research Unit-CNRST N°5, Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000, Marrakech, MoroccoLaboratory of Agri-Food, Biotechnologies and Valorization of Plant Bioresources (AGROBIOVAL). Agro Biotech Center, Labeled Research Unit-CNRST N°5, Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000, Marrakech, MoroccoLaboratory of Agri-Food, Biotechnologies and Valorization of Plant Bioresources (AGROBIOVAL). Agro Biotech Center, Labeled Research Unit-CNRST N°5, Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000, Marrakech, MoroccoLaboratory of Immunohistochemistry, Anatomic Pathology Department, University Hospital Center (CHU) Mohammed the VIth, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, MoroccoLaboratory of Immunohistochemistry, Anatomic Pathology Department, University Hospital Center (CHU) Mohammed the VIth, Faculty of Medicine and Pharmacy, Cadi Ayyad University, Marrakech, MoroccoDysphania ambrosioides (L.) holds a prominent place in Moroccan folk medicine due to its therapeutic attributes. Despite its widespread use, instances of inadvertent intoxication linked to its consumption have been reported. This study aims to evaluate the potential cytogenotoxic effects of D. ambrosioides leaf aqueous extract (DAAE) and explore the prospective protective role of vitamin C (l-ascorbic acid) through the micronucleus test conducted on (1) Vicia faba root-tip meristem and (2) mouse bone marrow cells. In addition, antioxidant enzyme activities, specifically superoxide dismutase (SOD) and catalase (CAT), were evaluated in V. faba treated with DAAE. After a 7-day daily administration of DAAE to mice, serum biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine, and uric acid) were measured, and histological examination of liver and kidney tissues was performed. The results indicated that DAAE had dose-dependent cytotoxic and genotoxic effects on both biological models. Furthermore, V. faba treated with DAAE showed significant increases in the activities of SOD and CAT enzymes. Mice treated with DAAE exhibited significant elevations in serum biochemical parameters compared to the control group. Histological examination of liver and kidney tissues revealed hepatic degeneration, glomerular shrinkage, and distinct vacuolated tubular epithelial cells. The cotreatment with vitamin C demonstrated a significant protective effect against DAAE-induced cytogenotoxicity. These findings underscore the importance of vitamin C as a protective agent against oxidative stress and cytogenotoxicity induced by DAAE and recommend its use in any DAAE-based preparation.https://doi.org/10.1515/chem-2023-0207dysphania ambrosioidesmicronucleusvicia fabaoxidative damagemice |
| spellingShingle | El-Bouzidi Laila khadra Ahmed Zefzoufi Manal Sissi Saida El-Abbassi Abdelilah Bekkouche Khalid Sellami Souad Rais Hanane In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract Open Chemistry dysphania ambrosioides micronucleus vicia faba oxidative damage mice |
| title | In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract |
| title_full | In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract |
| title_fullStr | In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract |
| title_full_unstemmed | In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract |
| title_short | In vivo protective effects of vitamin C against cyto-genotoxicity induced by Dysphania ambrosioides aqueous extract |
| title_sort | in vivo protective effects of vitamin c against cyto genotoxicity induced by dysphania ambrosioides aqueous extract |
| topic | dysphania ambrosioides micronucleus vicia faba oxidative damage mice |
| url | https://doi.org/10.1515/chem-2023-0207 |
| work_keys_str_mv | AT elbouzidilaila invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract AT khadraahmed invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract AT zefzoufimanal invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract AT sissisaida invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract AT elabbassiabdelilah invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract AT bekkouchekhalid invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract AT sellamisouad invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract AT raishanane invivoprotectiveeffectsofvitamincagainstcytogenotoxicityinducedbydysphaniaambrosioidesaqueousextract |