5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma
Gliomas typically have unfavorable prognosis, due to late detection and interventions. However, effective biomarkers for early glioma diagnosis based on 5-hydroxymethylcytosines (5 hm C) in circulating cell-free DNA (cfDNA) are not currently available. 5 hm C profiles in GSE132118 set were subjected...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022023106 |
| _version_ | 1828215207361511424 |
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| author | Chunyu Zhang Wei Zhou Yinqiu Tan Daofeng Tian Chunlong Zhong |
| author_facet | Chunyu Zhang Wei Zhou Yinqiu Tan Daofeng Tian Chunlong Zhong |
| author_sort | Chunyu Zhang |
| collection | DOAJ |
| description | Gliomas typically have unfavorable prognosis, due to late detection and interventions. However, effective biomarkers for early glioma diagnosis based on 5-hydroxymethylcytosines (5 hm C) in circulating cell-free DNA (cfDNA) are not currently available. 5 hm C profiles in GSE132118 set were subjected for establishment of diagnostic model using the LASSO (least absolute shrinkage and selection operator) algorithm. The 5 hm C-based models demonstrated great potency in differentiating healthy subjects from gliomas, with area under the curves (AUCs) > 0.91 in the training and validation sets. Moreover, the indicator performed well in combination with clinicopathological characteristics to differentiate glioblastomas (GBMs) from lower grade glioma (LGGs). Enrichment analysis on 5 hm C profiles displayed great correlation with glioma pathophysiology. The 5 hm C-derived biomarker might act as an effective and non-invasive measure in glioma screening. |
| first_indexed | 2024-04-12T15:17:36Z |
| format | Article |
| id | doaj.art-7bd663ebd360488a8decdb813c9b1d03 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-12T15:17:36Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-7bd663ebd360488a8decdb813c9b1d032022-12-22T03:27:35ZengElsevierHeliyon2405-84402022-10-01810e110225-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for gliomaChunyu Zhang0Wei Zhou1Yinqiu Tan2Daofeng Tian3Chunlong Zhong4Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Neurosurgery, Huzhou Central Hospital, Huzhou 313099, Zhejiang Province, ChinaDepartment of Anesthesiology, Huzhou Central Hospital, Huzhou 313099, Zhejiang Province, ChinaDepartment of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430060, Hubei Province, ChinaDepartment of Neurosurgery, Wuhan University, Renmin Hospital, Wuhan 430060, Hubei Province, China; Corresponding author.Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Corresponding author.Gliomas typically have unfavorable prognosis, due to late detection and interventions. However, effective biomarkers for early glioma diagnosis based on 5-hydroxymethylcytosines (5 hm C) in circulating cell-free DNA (cfDNA) are not currently available. 5 hm C profiles in GSE132118 set were subjected for establishment of diagnostic model using the LASSO (least absolute shrinkage and selection operator) algorithm. The 5 hm C-based models demonstrated great potency in differentiating healthy subjects from gliomas, with area under the curves (AUCs) > 0.91 in the training and validation sets. Moreover, the indicator performed well in combination with clinicopathological characteristics to differentiate glioblastomas (GBMs) from lower grade glioma (LGGs). Enrichment analysis on 5 hm C profiles displayed great correlation with glioma pathophysiology. The 5 hm C-derived biomarker might act as an effective and non-invasive measure in glioma screening.http://www.sciencedirect.com/science/article/pii/S2405844022023106Diagnostic biomarkercfDNA5 hm CGliomaLASSO |
| spellingShingle | Chunyu Zhang Wei Zhou Yinqiu Tan Daofeng Tian Chunlong Zhong 5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma Heliyon Diagnostic biomarker cfDNA 5 hm C Glioma LASSO |
| title | 5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma |
| title_full | 5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma |
| title_fullStr | 5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma |
| title_full_unstemmed | 5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma |
| title_short | 5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma |
| title_sort | 5 hydroxymethylcytosines in circulating cell free dna reveal a diagnostic biomarker for glioma |
| topic | Diagnostic biomarker cfDNA 5 hm C Glioma LASSO |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022023106 |
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