A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement
Genome editing is a relevant, versatile, and preferred tool for crop improvement, as well as for functional genomics. In this review, we summarize the advances in gene-editing techniques, such as zinc-finger nucleases (ZFNs), transcription activator-like (TAL) effector nucleases (TALENs), and cluste...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/16/5665 |
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| author | Sunny Ahmar Sumbul Saeed Muhammad Hafeez Ullah Khan Shahid Ullah Khan Freddy Mora-Poblete Muhammad Kamran Aroosha Faheem Ambreen Maqsood Muhammad Rauf Saba Saleem Woo-Jong Hong Ki-Hong Jung |
| author_facet | Sunny Ahmar Sumbul Saeed Muhammad Hafeez Ullah Khan Shahid Ullah Khan Freddy Mora-Poblete Muhammad Kamran Aroosha Faheem Ambreen Maqsood Muhammad Rauf Saba Saleem Woo-Jong Hong Ki-Hong Jung |
| author_sort | Sunny Ahmar |
| collection | DOAJ |
| description | Genome editing is a relevant, versatile, and preferred tool for crop improvement, as well as for functional genomics. In this review, we summarize the advances in gene-editing techniques, such as zinc-finger nucleases (ZFNs), transcription activator-like (TAL) effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) associated with the Cas9 and Cpf1 proteins. These tools support great opportunities for the future development of plant science and rapid remodeling of crops. Furthermore, we discuss the brief history of each tool and provide their comparison and different applications. Among the various genome-editing tools, CRISPR has become the most popular; hence, it is discussed in the greatest detail. CRISPR has helped clarify the genomic structure and its role in plants: For example, the transcriptional control of Cas9 and Cpf1, genetic locus monitoring, the mechanism and control of promoter activity, and the alteration and detection of epigenetic behavior between single-nucleotide polymorphisms (SNPs) investigated based on genetic traits and related genome-wide studies. The present review describes how CRISPR/Cas9 systems can play a valuable role in the characterization of the genomic rearrangement and plant gene functions, as well as the improvement of the important traits of field crops with the greatest precision. In addition, the speed editing strategy of gene-family members was introduced to accelerate the applications of gene-editing systems to crop improvement. For this, the CRISPR technology has a valuable advantage that particularly holds the scientist’s mind, as it allows genome editing in multiple biological systems. |
| first_indexed | 2024-03-10T17:48:56Z |
| format | Article |
| id | doaj.art-7bda8cac62e243a8b23623ff5fd26411 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:48:56Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7bda8cac62e243a8b23623ff5fd264112023-11-20T09:25:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116566510.3390/ijms21165665A Revolution toward Gene-Editing Technology and Its Application to Crop ImprovementSunny Ahmar0Sumbul Saeed1Muhammad Hafeez Ullah Khan2Shahid Ullah Khan3Freddy Mora-Poblete4Muhammad Kamran5Aroosha Faheem6Ambreen Maqsood7Muhammad Rauf8Saba Saleem9Woo-Jong Hong10Ki-Hong Jung11College of Plant Sciences and Technology Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Sciences and Technology Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Sciences and Technology Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Sciences and Technology Huazhong Agricultural University, Wuhan 430070, ChinaInstitute of Biological Sciences, University of Talca, 2 Norte 685, Talca 3460000, ChileKey Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, ChinaSate Key Laboratory of Agricultural Microbiology and State Key Laboratory of Microbial Biosensor, College of Life Sciences Huazhong Agriculture University Wuhan, Wuhan 430070, ChinaDepartment of Plant Pathology, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanNational Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad 38000, PakistanDepartment of Bioscience, COMSATS Institute of Information Technology, Islamabad 45550, PakistanGraduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, KoreaGraduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, KoreaGenome editing is a relevant, versatile, and preferred tool for crop improvement, as well as for functional genomics. In this review, we summarize the advances in gene-editing techniques, such as zinc-finger nucleases (ZFNs), transcription activator-like (TAL) effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR) associated with the Cas9 and Cpf1 proteins. These tools support great opportunities for the future development of plant science and rapid remodeling of crops. Furthermore, we discuss the brief history of each tool and provide their comparison and different applications. Among the various genome-editing tools, CRISPR has become the most popular; hence, it is discussed in the greatest detail. CRISPR has helped clarify the genomic structure and its role in plants: For example, the transcriptional control of Cas9 and Cpf1, genetic locus monitoring, the mechanism and control of promoter activity, and the alteration and detection of epigenetic behavior between single-nucleotide polymorphisms (SNPs) investigated based on genetic traits and related genome-wide studies. The present review describes how CRISPR/Cas9 systems can play a valuable role in the characterization of the genomic rearrangement and plant gene functions, as well as the improvement of the important traits of field crops with the greatest precision. In addition, the speed editing strategy of gene-family members was introduced to accelerate the applications of gene-editing systems to crop improvement. For this, the CRISPR technology has a valuable advantage that particularly holds the scientist’s mind, as it allows genome editing in multiple biological systems.https://www.mdpi.com/1422-0067/21/16/5665genome editingCRISPR/Cas9CRISPR/Cpf1TALENcrop improvementspeed editing strategy |
| spellingShingle | Sunny Ahmar Sumbul Saeed Muhammad Hafeez Ullah Khan Shahid Ullah Khan Freddy Mora-Poblete Muhammad Kamran Aroosha Faheem Ambreen Maqsood Muhammad Rauf Saba Saleem Woo-Jong Hong Ki-Hong Jung A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement International Journal of Molecular Sciences genome editing CRISPR/Cas9 CRISPR/Cpf1 TALEN crop improvement speed editing strategy |
| title | A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement |
| title_full | A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement |
| title_fullStr | A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement |
| title_full_unstemmed | A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement |
| title_short | A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement |
| title_sort | revolution toward gene editing technology and its application to crop improvement |
| topic | genome editing CRISPR/Cas9 CRISPR/Cpf1 TALEN crop improvement speed editing strategy |
| url | https://www.mdpi.com/1422-0067/21/16/5665 |
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