The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province
In order to investigate the effect of the type and concentration of solvent on the amount of extraction of phenolic compounds and antioxidant activity of Crataegus elbursensis leaves, a research was performed in two separate experiments. At first experiment the effect of solvent type (acetone, ethan...
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| Format: | Article |
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Islamic Azad University - Gorgan Branch
2022-12-01
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| Series: | فیزیولوژی محیطی گیاهی |
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| Online Access: | https://ecophysiologi.gorgan.iau.ir/article_679541_c7b6eb7c2f58a491f38e805ed82fc576.pdf |
| _version_ | 1797808139813257216 |
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| author | Mohammad Moghaddam Leila Mehdizade |
| author_facet | Mohammad Moghaddam Leila Mehdizade |
| author_sort | Mohammad Moghaddam |
| collection | DOAJ |
| description | In order to investigate the effect of the type and concentration of solvent on the amount of extraction of phenolic compounds and antioxidant activity of Crataegus elbursensis leaves, a research was performed in two separate experiments. At first experiment the effect of solvent type (acetone, ethanol, methanol) at three concentrations (50, 80 and 100%) on extracted total phenolics content of C. elbursensis leaf was evaluated. At second experiment antioxidant capacity of produced extract from the best solvent at first experiment with the highest phenolic compounds (methanol 80%) was investigated by two different methods including total antioxidant and Fe reduction capacity. The results of these experiments showed that all three solvents; acetone, ethanol and methanol; in the form of mixture with water have more potential for extracting phenolic compounds toward the pure ones. The highest total phenolic content (118 mg GAE/g DW) was obtained at 80% concentration of all solvents, especially at methanol 80%. The results of evaluating antioxidant activity showed that with increasing the concentration of extract up to 500 µg/mL, antioxidant activity (0.8 mg/mL) was increased. Investigation of Fe reduction capacity indicated that with increasing the concentration of extract up to 800 µg/mL, the amount of absorption of the solvents contain the extract significantly increased. Therefore, according to the results of this study, the leaves of this plant can be used as the source of phenolics and antioxidants in different industries. |
| first_indexed | 2024-03-13T06:33:04Z |
| format | Article |
| id | doaj.art-7be0b29b15da4dd7bc0daf449262e19c |
| institution | Directory Open Access Journal |
| issn | 2423-7671 2783-4689 |
| language | fas |
| last_indexed | 2024-03-13T06:33:04Z |
| publishDate | 2022-12-01 |
| publisher | Islamic Azad University - Gorgan Branch |
| record_format | Article |
| series | فیزیولوژی محیطی گیاهی |
| spelling | doaj.art-7be0b29b15da4dd7bc0daf449262e19c2023-06-09T06:42:02ZfasIslamic Azad University - Gorgan Branchفیزیولوژی محیطی گیاهی2423-76712783-46892022-12-0117689510810.30495/iper.2021.679541679541The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan provinceMohammad Moghaddam0Leila Mehdizade1Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.In order to investigate the effect of the type and concentration of solvent on the amount of extraction of phenolic compounds and antioxidant activity of Crataegus elbursensis leaves, a research was performed in two separate experiments. At first experiment the effect of solvent type (acetone, ethanol, methanol) at three concentrations (50, 80 and 100%) on extracted total phenolics content of C. elbursensis leaf was evaluated. At second experiment antioxidant capacity of produced extract from the best solvent at first experiment with the highest phenolic compounds (methanol 80%) was investigated by two different methods including total antioxidant and Fe reduction capacity. The results of these experiments showed that all three solvents; acetone, ethanol and methanol; in the form of mixture with water have more potential for extracting phenolic compounds toward the pure ones. The highest total phenolic content (118 mg GAE/g DW) was obtained at 80% concentration of all solvents, especially at methanol 80%. The results of evaluating antioxidant activity showed that with increasing the concentration of extract up to 500 µg/mL, antioxidant activity (0.8 mg/mL) was increased. Investigation of Fe reduction capacity indicated that with increasing the concentration of extract up to 800 µg/mL, the amount of absorption of the solvents contain the extract significantly increased. Therefore, according to the results of this study, the leaves of this plant can be used as the source of phenolics and antioxidants in different industries.https://ecophysiologi.gorgan.iau.ir/article_679541_c7b6eb7c2f58a491f38e805ed82fc576.pdfphenolic compoundssolventantioxidant capacitycrataegus elbursensisextract |
| spellingShingle | Mohammad Moghaddam Leila Mehdizade The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province فیزیولوژی محیطی گیاهی phenolic compounds solvent antioxidant capacity crataegus elbursensis extract |
| title | The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province |
| title_full | The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province |
| title_fullStr | The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province |
| title_full_unstemmed | The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province |
| title_short | The effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of Crataegus elbursensis L. leaf collected from Golestan province |
| title_sort | effect of solvent type and concentration on extraction of phenolic compounds and evaluation of antioxidant activity of crataegus elbursensis l leaf collected from golestan province |
| topic | phenolic compounds solvent antioxidant capacity crataegus elbursensis extract |
| url | https://ecophysiologi.gorgan.iau.ir/article_679541_c7b6eb7c2f58a491f38e805ed82fc576.pdf |
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