A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers
This paper describes the development of a biosensor based on label-free immunosensing for the detection of the C-terminal telopeptide bone turnover marker from type-1 collagen. A self-assembled monolayer (SAM) of dithiodipropionic acid was deposited on a gold electrode. Then streptavidin and biotiny...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2009-10-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/9/10/7957/ |
| _version_ | 1811264157471735808 |
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| author | Mark J. Schulz Amit Bhattacharya Yeo-Heung Yun Nelson B. Watts |
| author_facet | Mark J. Schulz Amit Bhattacharya Yeo-Heung Yun Nelson B. Watts |
| author_sort | Mark J. Schulz |
| collection | DOAJ |
| description | This paper describes the development of a biosensor based on label-free immunosensing for the detection of the C-terminal telopeptide bone turnover marker from type-1 collagen. A self-assembled monolayer (SAM) of dithiodipropionic acid was deposited on a gold electrode. Then streptavidin and biotinylated anti-human C-terminal telopeptide antibody were successively conjugated on the self-assembled monolayer. Electrochemical impedance measurements were made to characterize each step of the SAM/streptavidin/biotinylated antibody binding. Subsequently, electrochemical impedance was measured with different concentrations of C-teminal telopeptide. A detection limit of 50 ng/mL and a dynamic range up to 3 μg/mL were achieved. To our knowledge, this is the first attempt to develop a label-free immunosensor based on electrochemical impedance with DC bias for detection of bone-related degradation and rebuilding products. The electronic biosensor might eventually be used for quantitative point-of-care screening of bone health. It is hoped that analysis of bone turnover markers can indicate the beginning of bone diseases such as osteoarthritis and osteoporosis so that treatment might start early when it is most effective. |
| first_indexed | 2024-04-12T19:57:51Z |
| format | Article |
| id | doaj.art-7be24ca293c2431ebebd1aacb4d9fd42 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-12T19:57:51Z |
| publishDate | 2009-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-7be24ca293c2431ebebd1aacb4d9fd422022-12-22T03:18:36ZengMDPI AGSensors1424-82202009-10-019107957796910.3390/s91007957A Label-Free Electronic Biosensor for Detection of Bone Turnover MarkersMark J. SchulzAmit BhattacharyaYeo-Heung YunNelson B. WattsThis paper describes the development of a biosensor based on label-free immunosensing for the detection of the C-terminal telopeptide bone turnover marker from type-1 collagen. A self-assembled monolayer (SAM) of dithiodipropionic acid was deposited on a gold electrode. Then streptavidin and biotinylated anti-human C-terminal telopeptide antibody were successively conjugated on the self-assembled monolayer. Electrochemical impedance measurements were made to characterize each step of the SAM/streptavidin/biotinylated antibody binding. Subsequently, electrochemical impedance was measured with different concentrations of C-teminal telopeptide. A detection limit of 50 ng/mL and a dynamic range up to 3 μg/mL were achieved. To our knowledge, this is the first attempt to develop a label-free immunosensor based on electrochemical impedance with DC bias for detection of bone-related degradation and rebuilding products. The electronic biosensor might eventually be used for quantitative point-of-care screening of bone health. It is hoped that analysis of bone turnover markers can indicate the beginning of bone diseases such as osteoarthritis and osteoporosis so that treatment might start early when it is most effective.http://www.mdpi.com/1424-8220/9/10/7957/immunosensorlabel-freeelectrochemical impedancebone turnover marker |
| spellingShingle | Mark J. Schulz Amit Bhattacharya Yeo-Heung Yun Nelson B. Watts A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers Sensors immunosensor label-free electrochemical impedance bone turnover marker |
| title | A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers |
| title_full | A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers |
| title_fullStr | A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers |
| title_full_unstemmed | A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers |
| title_short | A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers |
| title_sort | label free electronic biosensor for detection of bone turnover markers |
| topic | immunosensor label-free electrochemical impedance bone turnover marker |
| url | http://www.mdpi.com/1424-8220/9/10/7957/ |
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