Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis Patients
Immunothrombosis, an excessive inflammatory response with simultaneous overactivation of the coagulation system, is a central pathomechanism in sepsis and COVID-19. It is associated with cellular activation, vascular damage, and microvascular thrombosis, which can lead to multiple organ failure and...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/6/1069 |
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| author | Tanja Eichhorn Silke Huber René Weiss Marie Ebeyer-Masotta Lucia Lauková Robert Emprechtinger Rosa Bellmann-Weiler Ingo Lorenz Judith Martini Markus Pirklbauer Dorothea Orth-Höller Reinhard Würzner Viktoria Weber |
| author_facet | Tanja Eichhorn Silke Huber René Weiss Marie Ebeyer-Masotta Lucia Lauková Robert Emprechtinger Rosa Bellmann-Weiler Ingo Lorenz Judith Martini Markus Pirklbauer Dorothea Orth-Höller Reinhard Würzner Viktoria Weber |
| author_sort | Tanja Eichhorn |
| collection | DOAJ |
| description | Immunothrombosis, an excessive inflammatory response with simultaneous overactivation of the coagulation system, is a central pathomechanism in sepsis and COVID-19. It is associated with cellular activation, vascular damage, and microvascular thrombosis, which can lead to multiple organ failure and death. Here, we characterized factors related to immunothrombosis in plasma samples from 78 sepsis patients. In the course of routine clinical testing, SARS-CoV-2 was detected in 14 of these patients. Viral infection was associated with a higher mortality. Both, COVID-19 negative and COVID-19 positive sepsis patients showed increased levels of effectors of immunothrombosis, including platelet factor 4, D-dimer, nucleosomes, citrullinated histone H3, high mobility group box-1 protein, as well as phosphatidylserine-expressing platelet-derived extracellular vesicles, compared to healthy controls (<i>n</i> = 25). Using a 27-plex cytokine bead array, we found that Interleukin (IL)-1ra, IL-6, IL-8, IL-13, tumor necrosis factor (TNF)-α, interferon inducible protein (IP)-10, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-1α, and granulocyte-colony stimulating factor (G-CSF) were elevated in both, COVID-19 negative and COVID-19 positive sepsis patients, as compared to healthy controls. SARS-CoV-2 infection was associated with elevated levels of IP-10, MCP-1, and IL-13, while all other mediators widely overlapped between COVID-19 negative and COVID-19 positive patients. |
| first_indexed | 2024-03-11T06:41:53Z |
| format | Article |
| id | doaj.art-7be50c8cb413442eaf2a54dc87525160 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T06:41:53Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-7be50c8cb413442eaf2a54dc875251602023-11-17T10:33:55ZengMDPI AGDiagnostics2075-44182023-03-01136106910.3390/diagnostics13061069Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis PatientsTanja Eichhorn0Silke Huber1René Weiss2Marie Ebeyer-Masotta3Lucia Lauková4Robert Emprechtinger5Rosa Bellmann-Weiler6Ingo Lorenz7Judith Martini8Markus Pirklbauer9Dorothea Orth-Höller10Reinhard Würzner11Viktoria Weber12Department for Biomedical Research, University for Continuing Education Krems, 3500 Krems, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, AustriaDepartment for Biomedical Research, University for Continuing Education Krems, 3500 Krems, AustriaDepartment for Biomedical Research, University for Continuing Education Krems, 3500 Krems, AustriaDepartment for Biomedical Research, University for Continuing Education Krems, 3500 Krems, AustriaFaculty of Health and Medicine, University for Continuing Education Krems, 3500 Krems, AustriaDepartment of Internal Medicine II, Medical University of Innsbruck, 6020 Innsbruck, AustriaDepartment of General and Surgical Critical Care Medicine, Medical University of Innsbruck, 6020 Innsbruck, AustriaDepartment of Anaesthesia and Intensive Care Medicine, Medical University of Innsbruck, 6020 Innsbruck, AustriaDepartment of Internal Medicine IV-Nephrology and Hypertension, Medical University Innsbruck, 6020 Innsbruck, AustriaMB-LAB Clinical Microbiology Laboratory, 6020 Innsbruck, AustriaInstitute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, AustriaDepartment for Biomedical Research, University for Continuing Education Krems, 3500 Krems, AustriaImmunothrombosis, an excessive inflammatory response with simultaneous overactivation of the coagulation system, is a central pathomechanism in sepsis and COVID-19. It is associated with cellular activation, vascular damage, and microvascular thrombosis, which can lead to multiple organ failure and death. Here, we characterized factors related to immunothrombosis in plasma samples from 78 sepsis patients. In the course of routine clinical testing, SARS-CoV-2 was detected in 14 of these patients. Viral infection was associated with a higher mortality. Both, COVID-19 negative and COVID-19 positive sepsis patients showed increased levels of effectors of immunothrombosis, including platelet factor 4, D-dimer, nucleosomes, citrullinated histone H3, high mobility group box-1 protein, as well as phosphatidylserine-expressing platelet-derived extracellular vesicles, compared to healthy controls (<i>n</i> = 25). Using a 27-plex cytokine bead array, we found that Interleukin (IL)-1ra, IL-6, IL-8, IL-13, tumor necrosis factor (TNF)-α, interferon inducible protein (IP)-10, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-1α, and granulocyte-colony stimulating factor (G-CSF) were elevated in both, COVID-19 negative and COVID-19 positive sepsis patients, as compared to healthy controls. SARS-CoV-2 infection was associated with elevated levels of IP-10, MCP-1, and IL-13, while all other mediators widely overlapped between COVID-19 negative and COVID-19 positive patients.https://www.mdpi.com/2075-4418/13/6/1069sepsisCOVID-19immunothrombosisextracellular vesiclesinflammation |
| spellingShingle | Tanja Eichhorn Silke Huber René Weiss Marie Ebeyer-Masotta Lucia Lauková Robert Emprechtinger Rosa Bellmann-Weiler Ingo Lorenz Judith Martini Markus Pirklbauer Dorothea Orth-Höller Reinhard Würzner Viktoria Weber Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis Patients Diagnostics sepsis COVID-19 immunothrombosis extracellular vesicles inflammation |
| title | Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis Patients |
| title_full | Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis Patients |
| title_fullStr | Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis Patients |
| title_full_unstemmed | Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis Patients |
| title_short | Infection with SARS-CoV-2 Is Associated with Elevated Levels of IP-10, MCP-1, and IL-13 in Sepsis Patients |
| title_sort | infection with sars cov 2 is associated with elevated levels of ip 10 mcp 1 and il 13 in sepsis patients |
| topic | sepsis COVID-19 immunothrombosis extracellular vesicles inflammation |
| url | https://www.mdpi.com/2075-4418/13/6/1069 |
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