Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, Durgapur
It is widely acknowledged that conventional mining and extraction techniques have left many parts of the world with depleting coal reserves. A sustainable method for improving the recovery of natural gas from coalbeds involves enhancing the production of biogenic methane in coal mines. By taking a c...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233605/full |
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| author | Mansi Chawla Meeta Lavania Nishi Sahu Sudhanshu Shekhar Nimmi Singh Anand More Magesh Iyer Sanjay Kumar Komal Singh Banwari Lal |
| author_facet | Mansi Chawla Meeta Lavania Nishi Sahu Sudhanshu Shekhar Nimmi Singh Anand More Magesh Iyer Sanjay Kumar Komal Singh Banwari Lal |
| author_sort | Mansi Chawla |
| collection | DOAJ |
| description | It is widely acknowledged that conventional mining and extraction techniques have left many parts of the world with depleting coal reserves. A sustainable method for improving the recovery of natural gas from coalbeds involves enhancing the production of biogenic methane in coal mines. By taking a culture-independent approach, the diversity of the microbial community present in the formation water of an Indian reservoir was examined using 16S rRNA gene amplification in order to study the potential of microbial-enhanced coal bed methane (CBM) production from the deep thermogenic wells at a depth of 800–1200 m. Physicochemical characterization of formation water and coal samples was performed with the aim of understanding the in situ reservoir conditions that are most favorable for microbial CBM production. Microbial community analysis of formation water showed that bacteria were more abundant than archaea. Proteobacteria, Firmicutes, and Bacteroidetes were found as the most prevalent phyla in all the samples. These phyla play a crucial role in providing substrate for the process of methanogenesis by performing fermentative, hydrolytic, and syntrophic functions. Considerable variation in the abundance of microbial genera was observed amongst the selected CBM wells, potentially due to variable local geochemical conditions within the reservoir. The results of our study provide insights into the impact of geochemical factors on microbial distribution within the reservoir. Further, the study demonstrates lab-scale enhancement in methane production through nutrient amendment. It also focuses on understanding the microbial diversity of the Raniganj coalbed methane block using amplicon sequencing and further recognizing the potential of biogenic methane enhancement through microbial stimulation. The findings of the study will help as a reference for better strategization and implementation of on-site microbial stimulation for enhanced biogenic methane production in the future. |
| first_indexed | 2024-03-12T02:37:20Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T02:37:20Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7be54fc775f146839f81548a0b36749b2023-09-04T13:55:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12336051233605Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, DurgapurMansi Chawla0Meeta Lavania1Nishi Sahu2Sudhanshu Shekhar3Nimmi Singh4Anand More5Magesh Iyer6Sanjay Kumar7Komal Singh8Banwari Lal9Environmental and Industrial Biotechnology Division, The Energy and Resources Institute, New Delhi, IndiaEnvironmental and Industrial Biotechnology Division, The Energy and Resources Institute, New Delhi, IndiaEnvironmental and Industrial Biotechnology Division, The Energy and Resources Institute, New Delhi, IndiaONGC Energy Centre, New Delhi, IndiaEnvironmental and Industrial Biotechnology Division, The Energy and Resources Institute, New Delhi, IndiaEssar Oil and Gas Exploration and Production Limited, Durgapur, West Bengal, IndiaEssar Oil and Gas Exploration and Production Limited, Durgapur, West Bengal, IndiaEssar Oil and Gas Exploration and Production Limited, Durgapur, West Bengal, IndiaONGC Energy Centre, New Delhi, IndiaEnvironmental and Industrial Biotechnology Division, The Energy and Resources Institute, New Delhi, IndiaIt is widely acknowledged that conventional mining and extraction techniques have left many parts of the world with depleting coal reserves. A sustainable method for improving the recovery of natural gas from coalbeds involves enhancing the production of biogenic methane in coal mines. By taking a culture-independent approach, the diversity of the microbial community present in the formation water of an Indian reservoir was examined using 16S rRNA gene amplification in order to study the potential of microbial-enhanced coal bed methane (CBM) production from the deep thermogenic wells at a depth of 800–1200 m. Physicochemical characterization of formation water and coal samples was performed with the aim of understanding the in situ reservoir conditions that are most favorable for microbial CBM production. Microbial community analysis of formation water showed that bacteria were more abundant than archaea. Proteobacteria, Firmicutes, and Bacteroidetes were found as the most prevalent phyla in all the samples. These phyla play a crucial role in providing substrate for the process of methanogenesis by performing fermentative, hydrolytic, and syntrophic functions. Considerable variation in the abundance of microbial genera was observed amongst the selected CBM wells, potentially due to variable local geochemical conditions within the reservoir. The results of our study provide insights into the impact of geochemical factors on microbial distribution within the reservoir. Further, the study demonstrates lab-scale enhancement in methane production through nutrient amendment. It also focuses on understanding the microbial diversity of the Raniganj coalbed methane block using amplicon sequencing and further recognizing the potential of biogenic methane enhancement through microbial stimulation. The findings of the study will help as a reference for better strategization and implementation of on-site microbial stimulation for enhanced biogenic methane production in the future.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233605/fullcoalbed methane (CBM)methanogenesismicrobial communitybiogenic CBM enhancementRaniganj coal block |
| spellingShingle | Mansi Chawla Meeta Lavania Nishi Sahu Sudhanshu Shekhar Nimmi Singh Anand More Magesh Iyer Sanjay Kumar Komal Singh Banwari Lal Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, Durgapur Frontiers in Microbiology coalbed methane (CBM) methanogenesis microbial community biogenic CBM enhancement Raniganj coal block |
| title | Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, Durgapur |
| title_full | Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, Durgapur |
| title_fullStr | Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, Durgapur |
| title_full_unstemmed | Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, Durgapur |
| title_short | Culture-independent assessment of the indigenous microbial diversity of Raniganj coal bed methane block, Durgapur |
| title_sort | culture independent assessment of the indigenous microbial diversity of raniganj coal bed methane block durgapur |
| topic | coalbed methane (CBM) methanogenesis microbial community biogenic CBM enhancement Raniganj coal block |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233605/full |
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