Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation
The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlled conditions to guarantee the efficiency of in vitro propagation with plants fre...
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| Format: | Article |
| Language: | English |
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Universidad Central Marta Abreu de Las Villas
2021-03-01
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| Series: | Biotecnología Vegetal |
| Subjects: | |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/692 |
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| author | Pablo Machado Armas Dunia Núñez Jaramillo Edel Alejandro Toledo Rodríguez Rafael Gómez Kosky Aldo Sergio Noguera Atilio Pedro Castagnaro |
| author_facet | Pablo Machado Armas Dunia Núñez Jaramillo Edel Alejandro Toledo Rodríguez Rafael Gómez Kosky Aldo Sergio Noguera Atilio Pedro Castagnaro |
| author_sort | Pablo Machado Armas |
| collection | DOAJ |
| description | The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlled conditions to guarantee the efficiency of in vitro propagation with plants free of Xanthomonas albilineans. A greenhouse was modified and the structure was remodeled, a Sarán shade mesh (50%) and a sprinkler irrigation system were placed. The plants were grown in plastic containers (20 m3 volume) with a mixture of cachaça compost and zeolite 3:1. Stems of cultivars C98-357, C97-445 and C86-156, without symptoms of leaf scald and with six months of culture, were cut into segments with a bud. Hydrothermal treatment was applied and subsequently the biostimulant VIUSID Agro® (0.8 ml l-1) before the buds planting. At three months of culture, a serological diagnosis was made to detect the presence of X. albilineans. The negative plants were treated with biostimulants and the response of the in vitro establishment was evaluated. Adequate plant growth was achieved under the conditions described. The establishment of a bank of sugarcane mother plants under semi-controlled conditions and its management guaranteed that microbial contamination and phenolic oxidation decreased in the in vitro establishment phase. The effectiveness of the hydrothermal treatment combined with the semi-controlled conditions and the management of the plants make it possible to reduce the incidence of X. albilineans. |
| first_indexed | 2024-12-23T10:00:30Z |
| format | Article |
| id | doaj.art-7be63c38a67a41e2befe667c47482f58 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-23T10:00:30Z |
| publishDate | 2021-03-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-7be63c38a67a41e2befe667c47482f582022-12-21T17:51:15ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472021-03-012115361Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation Pablo Machado Armas0Dunia Núñez Jaramillo1 Edel Alejandro Toledo Rodríguez2https://orcid.org/0000-0002-0522-7330Rafael Gómez Kosky3https://orcid.org/0000-0003-3656-9824 Aldo Sergio Noguera4https://orcid.org/0000-0003-2768-5671 Atilio Pedro Castagnaro5https://orcid.org/0000-0002-1599-2255Estación Territorial de Investigaciones de la Caña de Azúcar (ETICA) Centro-Villa Clara. Autopista nacional km 246. Ranchuelo. Villa Clara. Cuba. CP 53100.Estación Territorial de Investigaciones de la Caña de Azúcar (ETICA) Centro-Villa Clara. Autopista nacional km 246. Ranchuelo. Villa Clara. Cuba. CP 53100.Estación Territorial de Investigaciones de la Caña de Azúcar (ETICA) Centro-Villa Clara. Autopista nacional km 246. Ranchuelo. Villa Clara. Cuba. CP 53100.Estación Territorial de Investigaciones de la Caña de Azúcar (ETICA) Centro-Villa Clara. Autopista nacional km 246. Ranchuelo. Villa Clara. Cuba. CP 53100.Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Estación Experimental Agroindustrial Obispo Colombres (EEAOC) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Tucumán. Argentina.Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITANOA), Estación Experimental Agroindustrial Obispo Colombres (EEAOC) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Tucumán. Argentina.The propagation of sugarcane (Saccharum spp.) Requires seed with physiological and phytosanitary quality. The work was carried out with the objective of establishing a bank of sugarcane mother plants under semi-controlled conditions to guarantee the efficiency of in vitro propagation with plants free of Xanthomonas albilineans. A greenhouse was modified and the structure was remodeled, a Sarán shade mesh (50%) and a sprinkler irrigation system were placed. The plants were grown in plastic containers (20 m3 volume) with a mixture of cachaça compost and zeolite 3:1. Stems of cultivars C98-357, C97-445 and C86-156, without symptoms of leaf scald and with six months of culture, were cut into segments with a bud. Hydrothermal treatment was applied and subsequently the biostimulant VIUSID Agro® (0.8 ml l-1) before the buds planting. At three months of culture, a serological diagnosis was made to detect the presence of X. albilineans. The negative plants were treated with biostimulants and the response of the in vitro establishment was evaluated. Adequate plant growth was achieved under the conditions described. The establishment of a bank of sugarcane mother plants under semi-controlled conditions and its management guaranteed that microbial contamination and phenolic oxidation decreased in the in vitro establishment phase. The effectiveness of the hydrothermal treatment combined with the semi-controlled conditions and the management of the plants make it possible to reduce the incidence of X. albilineans.https://revista.ibp.co.cu/index.php/BV/article/view/692in vitro culturefoliar scaldxanthomonas albilineans |
| spellingShingle | Pablo Machado Armas Dunia Núñez Jaramillo Edel Alejandro Toledo Rodríguez Rafael Gómez Kosky Aldo Sergio Noguera Atilio Pedro Castagnaro Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation Biotecnología Vegetal in vitro culture foliar scald xanthomonas albilineans |
| title | Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation |
| title_full | Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation |
| title_fullStr | Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation |
| title_full_unstemmed | Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation |
| title_short | Establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation |
| title_sort | establishment of sugarcane mother plant bank in semicontroled conditions for in vitro propagation |
| topic | in vitro culture foliar scald xanthomonas albilineans |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/692 |
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