Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis
The effectivity of employing two types of cellulases from Aspergillus niger and Trichoderma resei covalently immobilized on chitosan microparticle was investigated. Reducing sugar from CMC yielded by immobilized cellulase from T. resei alone and A. niger alone was 0.316 g/L and 0.244 g/L, respective...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2019-04-01
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| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/39714 |
| _version_ | 1818836557531971584 |
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| author | Afan Hamzah Lidya Lorenta Sitompul Irma Nurhanifah Fenda Putri Soeprijanto Soeprijanto Arief Widjaja |
| author_facet | Afan Hamzah Lidya Lorenta Sitompul Irma Nurhanifah Fenda Putri Soeprijanto Soeprijanto Arief Widjaja |
| author_sort | Afan Hamzah |
| collection | DOAJ |
| description | The effectivity of employing two types of cellulases from Aspergillus niger and Trichoderma resei covalently immobilized on chitosan microparticle was investigated. Reducing sugar from CMC yielded by immobilized cellulase from T. resei alone and A. niger alone was 0.316 g/L and 0.244 g/L, respectively. Simultaneous use of both cellulases shows a significant increase of reducing sugar produced to 1.020 g/L. The effective combination of this two types of cellulases also occurred when coconut husk was used as substrate. A very high enzyme coupling of 92.06% compared to free enzyme was obtained in the immobilization. Addition of GDA not only increased enzyme coupling to 100% but also improved sugar produced. Immobilized cellulase was successfully maintained its activity until 5 cycles |
| first_indexed | 2024-12-19T03:08:30Z |
| format | Article |
| id | doaj.art-7be6948e36204ed1a23e5dda7803ba07 |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-19T03:08:30Z |
| publishDate | 2019-04-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-7be6948e36204ed1a23e5dda7803ba072022-12-21T20:38:04ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782019-04-0119249550210.22146/ijc.3971423295Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk HydrolysisAfan Hamzah0Lidya Lorenta Sitompul1Irma Nurhanifah Fenda Putri2Soeprijanto Soeprijanto3Arief Widjaja4Department of Chemical Engineering, Institut Teknologi Sepuluh NopemberDepartment of Chemical Engineering, Institut Teknologi Sepuluh NopemberDepartment of Chemical Engineering, Institut Teknologi Sepuluh NopemberDepartment of Chemical Engineering, Institut Teknologi Sepuluh NopemberDepartment of Chemical Engineering, Institut Teknologi Sepuluh NopemberThe effectivity of employing two types of cellulases from Aspergillus niger and Trichoderma resei covalently immobilized on chitosan microparticle was investigated. Reducing sugar from CMC yielded by immobilized cellulase from T. resei alone and A. niger alone was 0.316 g/L and 0.244 g/L, respectively. Simultaneous use of both cellulases shows a significant increase of reducing sugar produced to 1.020 g/L. The effective combination of this two types of cellulases also occurred when coconut husk was used as substrate. A very high enzyme coupling of 92.06% compared to free enzyme was obtained in the immobilization. Addition of GDA not only increased enzyme coupling to 100% but also improved sugar produced. Immobilized cellulase was successfully maintained its activity until 5 cycleshttps://jurnal.ugm.ac.id/ijc/article/view/39714chitosancellulaseimmobilizationmicroparticlesynergycoconut husk |
| spellingShingle | Afan Hamzah Lidya Lorenta Sitompul Irma Nurhanifah Fenda Putri Soeprijanto Soeprijanto Arief Widjaja Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis Indonesian Journal of Chemistry chitosan cellulase immobilization microparticle synergy coconut husk |
| title | Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis |
| title_full | Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis |
| title_fullStr | Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis |
| title_full_unstemmed | Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis |
| title_short | Synergistic Effect of Two Type Cellulase Immobilized on Chitosan Microparticle as Biocatalyst for Coconut Husk Hydrolysis |
| title_sort | synergistic effect of two type cellulase immobilized on chitosan microparticle as biocatalyst for coconut husk hydrolysis |
| topic | chitosan cellulase immobilization microparticle synergy coconut husk |
| url | https://jurnal.ugm.ac.id/ijc/article/view/39714 |
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