Quantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS)

Quantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS)

Lipids, such as phosphoinositides (PIPs) and diacylglycerol (DAG), are important signaling intermediates involved in cellular processes such as T cell receptor (TCR)-mediated signal transduction. Here we report identification and quantification of PIP, PIP2 and DAG from crude lipid extracts. Capital...

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Bibliographic Details
Main Authors: Mathias Haag, Angelika Schmidt, Timo Sachsenheimer, Britta Brügger
Format: Article
Language:English
Published: MDPI AG 2012-01-01
Series:Metabolites
Subjects:
T cell signaling
signaling lipids
diacylglycerol
phosphoinositides
lipidomics
nano-electrospray ionization tandem mass spectrometry
Online Access:http://www.mdpi.com/2218-1989/2/1/57

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