Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China
Porcine reproductive and respiratory syndrome virus (PRRSV) has brought serious economic losses to pig industry. PRRSV-1 have existed in China for more than 25 years. The prevalence and features of PRRSV-1 on Chinese farms are unclear. We continuously monitored PRRSV in a pig farm with strict biosaf...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1067173/full |
| _version_ | 1811321019162427392 |
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| author | Chao Li Hu Xu Jing Zhao Bangjun Gong Qi Sun Lirun Xiang Wansheng Li Zhenyang Guo Jinhao Li Yan-dong Tang Chaoliang Leng Jinmei Peng Qian Wang Tongqing An Xuehui Cai Zhi-Jun Tian Guohui Zhou Hongliang Zhang |
| author_facet | Chao Li Hu Xu Jing Zhao Bangjun Gong Qi Sun Lirun Xiang Wansheng Li Zhenyang Guo Jinhao Li Yan-dong Tang Chaoliang Leng Jinmei Peng Qian Wang Tongqing An Xuehui Cai Zhi-Jun Tian Guohui Zhou Hongliang Zhang |
| author_sort | Chao Li |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) has brought serious economic losses to pig industry. PRRSV-1 have existed in China for more than 25 years. The prevalence and features of PRRSV-1 on Chinese farms are unclear. We continuously monitored PRRSV in a pig farm with strict biosafety measures in Henan Province, China, in 2020. The results showed that multiple types of PRRSV coexisted on this single pig farm. PRRSV-1 was one of the main circulating strains on the farm and was responsible for infections throughout nearly the entire epidemic cycle. Phylogenetic analysis showed that PRRSV-1 isolates from this pig farm formed an independent branch, with all isolates belonging to BJEU06-1-like PRRSV. The analysis of selection pressure on ORF5 on this branch identified 5 amino acids as positive selection sites, indicating that PRRSV-1 had undergone adaptive evolution on this farm. According to the analysis of ORF5 of PRRSV-1 on this farm, the evolutionary rate of the BJEU06-1-like branch was estimated to be 1.01 × 10−2 substitutions/site/year. To further understand the genome-wide characteristics of PRRSV-1 on this pig farm, two full-length PRRSV-1 genomes representative of pig farms were obtained. The results of amino acid alignment revealed that although one NSP2 deletion was consistent with BJEU06-1, different new features were found in ORF3 and ORF4. According to the above results, PRRSV-1 has undergone considerable evolution in China. This study is the first to report the prevalence and characteristics of PRRSV-1 on a large farm in mainland China, which will provide a reference for the identification and further prevention and control of PRRSV-1. |
| first_indexed | 2024-04-13T13:09:38Z |
| format | Article |
| id | doaj.art-7be9db5e708140199167e95467a449f2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T13:09:38Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7be9db5e708140199167e95467a449f22022-12-22T02:45:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10671731067173Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in ChinaChao Li0Hu Xu1Jing Zhao2Bangjun Gong3Qi Sun4Lirun Xiang5Wansheng Li6Zhenyang Guo7Jinhao Li8Yan-dong Tang9Chaoliang Leng10Jinmei Peng11Qian Wang12Tongqing An13Xuehui Cai14Zhi-Jun Tian15Guohui Zhou16Hongliang Zhang17State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaHenan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bio-Reactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaPorcine reproductive and respiratory syndrome virus (PRRSV) has brought serious economic losses to pig industry. PRRSV-1 have existed in China for more than 25 years. The prevalence and features of PRRSV-1 on Chinese farms are unclear. We continuously monitored PRRSV in a pig farm with strict biosafety measures in Henan Province, China, in 2020. The results showed that multiple types of PRRSV coexisted on this single pig farm. PRRSV-1 was one of the main circulating strains on the farm and was responsible for infections throughout nearly the entire epidemic cycle. Phylogenetic analysis showed that PRRSV-1 isolates from this pig farm formed an independent branch, with all isolates belonging to BJEU06-1-like PRRSV. The analysis of selection pressure on ORF5 on this branch identified 5 amino acids as positive selection sites, indicating that PRRSV-1 had undergone adaptive evolution on this farm. According to the analysis of ORF5 of PRRSV-1 on this farm, the evolutionary rate of the BJEU06-1-like branch was estimated to be 1.01 × 10−2 substitutions/site/year. To further understand the genome-wide characteristics of PRRSV-1 on this pig farm, two full-length PRRSV-1 genomes representative of pig farms were obtained. The results of amino acid alignment revealed that although one NSP2 deletion was consistent with BJEU06-1, different new features were found in ORF3 and ORF4. According to the above results, PRRSV-1 has undergone considerable evolution in China. This study is the first to report the prevalence and characteristics of PRRSV-1 on a large farm in mainland China, which will provide a reference for the identification and further prevention and control of PRRSV-1.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1067173/fullPRRSV-1first detectionmain epidemic strainChinese pig farmevolution and genetic diversity |
| spellingShingle | Chao Li Hu Xu Jing Zhao Bangjun Gong Qi Sun Lirun Xiang Wansheng Li Zhenyang Guo Jinhao Li Yan-dong Tang Chaoliang Leng Jinmei Peng Qian Wang Tongqing An Xuehui Cai Zhi-Jun Tian Guohui Zhou Hongliang Zhang Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China Frontiers in Microbiology PRRSV-1 first detection main epidemic strain Chinese pig farm evolution and genetic diversity |
| title | Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China |
| title_full | Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China |
| title_fullStr | Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China |
| title_full_unstemmed | Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China |
| title_short | Epidemiological investigation and genetic evolutionary analysis of PRRSV-1 on a pig farm in China |
| title_sort | epidemiological investigation and genetic evolutionary analysis of prrsv 1 on a pig farm in china |
| topic | PRRSV-1 first detection main epidemic strain Chinese pig farm evolution and genetic diversity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1067173/full |
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