Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549
Objective To explore mechanism of prohibitin 2(PHB2) inhibition on the apoptosis of non-small cell lung cancer cell line A549. Methods For stable knockdown of PHB2, A549 strain was infected with lentiviruses. A549 cells with PHB2 depletion were incubated with Mdivi-1(DRP1 inhibitor). Apopt...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-10-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1522.pdf |
| _version_ | 1827386931109953536 |
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| author | ZHANG Jing, YANG Zigeng, WEI Hongmei, NING Haihong, XUE Xixi, JIN Ling, WU Bin |
| author_facet | ZHANG Jing, YANG Zigeng, WEI Hongmei, NING Haihong, XUE Xixi, JIN Ling, WU Bin |
| author_sort | ZHANG Jing, YANG Zigeng, WEI Hongmei, NING Haihong, XUE Xixi, JIN Ling, WU Bin |
| collection | DOAJ |
| description | Objective To explore mechanism of prohibitin 2(PHB2) inhibition on the apoptosis of non-small cell lung cancer cell line A549. Methods For stable knockdown of PHB2, A549 strain was infected with lentiviruses. A549 cells with PHB2 depletion were incubated with Mdivi-1(DRP1 inhibitor). Apoptotic index of A549 cells was evaluated by TUNEL and annexin V-FITC/PI. Mitochondrial morphology was visualized by MitoTracker. ATP content, citrate synthase activity and the level of cytochrome c in cytosolic and mitochondrial fractions were analyzed by commercially available kits. Western blot was used to measure the levels of PHB2, DRP1 and p-DRP1 protein expression. Results Compared with the shCtrl group, the apoptosis of A549 cells increased significantly (P＜0.05) with up-regulated DRP1-dependent mitochondrial fission (P＜0.05), down-regulated ATP content (P＜0.05) and citrate synthase activity (P＜0.05). The level of cytochrome C was decreased in mitochondrial fractions (P＜0.05). Compared with the shPHB2 group, the apoptosis of A549 cells decreased significantly (P＜0.05) with alleviated DRP1-dependent mitochondrial fission (P＜0.05), increase of ATP content (P＜0.05) and citrate synthase activity(P＜0.05). The level of cytochrome C was increased in mitochondrial fractions (P＜0.05) in the shPHB2+Mdivi-1 group. Conclusions PHB2 inhibition can significantly enhance the apoptosis rate of A549 cells, potentially through promoting DRP1-dependent mitochondrial fission. |
| first_indexed | 2024-03-08T15:53:42Z |
| format | Article |
| id | doaj.art-7be9ecdf97c442cd883e85783f44a80e |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T15:53:42Z |
| publishDate | 2023-10-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-7be9ecdf97c442cd883e85783f44a80e2024-01-09T03:15:35ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-10-0143101522152910.16352/j.issn.1001-6325.2023.10.1522Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549ZHANG Jing, YANG Zigeng, WEI Hongmei, NING Haihong, XUE Xixi, JIN Ling, WU Bin01. Department of Nutrition;;2. Department of Nuclear Medicine;;3. Training Center of Health Service;;4. Department of Geriatrics,General Hospital of Xinjiang Military Command, Urumqi 830000, ChinaObjective To explore mechanism of prohibitin 2(PHB2) inhibition on the apoptosis of non-small cell lung cancer cell line A549. Methods For stable knockdown of PHB2, A549 strain was infected with lentiviruses. A549 cells with PHB2 depletion were incubated with Mdivi-1(DRP1 inhibitor). Apoptotic index of A549 cells was evaluated by TUNEL and annexin V-FITC/PI. Mitochondrial morphology was visualized by MitoTracker. ATP content, citrate synthase activity and the level of cytochrome c in cytosolic and mitochondrial fractions were analyzed by commercially available kits. Western blot was used to measure the levels of PHB2, DRP1 and p-DRP1 protein expression. Results Compared with the shCtrl group, the apoptosis of A549 cells increased significantly (P＜0.05) with up-regulated DRP1-dependent mitochondrial fission (P＜0.05), down-regulated ATP content (P＜0.05) and citrate synthase activity (P＜0.05). The level of cytochrome C was decreased in mitochondrial fractions (P＜0.05). Compared with the shPHB2 group, the apoptosis of A549 cells decreased significantly (P＜0.05) with alleviated DRP1-dependent mitochondrial fission (P＜0.05), increase of ATP content (P＜0.05) and citrate synthase activity(P＜0.05). The level of cytochrome C was increased in mitochondrial fractions (P＜0.05) in the shPHB2+Mdivi-1 group. Conclusions PHB2 inhibition can significantly enhance the apoptosis rate of A549 cells, potentially through promoting DRP1-dependent mitochondrial fission.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1522.pdfprohibitin 2(phb2)|mitochondrial fission|non-small cell lung cancer|dynamin-related protein 1(drp1)|apoptosis |
| spellingShingle | ZHANG Jing, YANG Zigeng, WEI Hongmei, NING Haihong, XUE Xixi, JIN Ling, WU Bin Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549 Jichu yixue yu linchuang prohibitin 2(phb2)|mitochondrial fission|non-small cell lung cancer|dynamin-related protein 1(drp1)|apoptosis |
| title | Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549 |
| title_full | Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549 |
| title_fullStr | Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549 |
| title_full_unstemmed | Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549 |
| title_short | Knockdown of prohibitin 2 promotes apoptosis in non-small cell lung cancer cell line A549 |
| title_sort | knockdown of prohibitin 2 promotes apoptosis in non small cell lung cancer cell line a549 |
| topic | prohibitin 2(phb2)|mitochondrial fission|non-small cell lung cancer|dynamin-related protein 1(drp1)|apoptosis |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-10-1522.pdf |
| work_keys_str_mv | AT zhangjingyangzigengweihongmeininghaihongxuexixijinlingwubin knockdownofprohibitin2promotesapoptosisinnonsmallcelllungcancercelllinea549 |