Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cells
This study was undertaken in order to assess whether triglycerides and/or their fatty acids directly influence the secretion of plasminogen activator inhibitor type 1 (PAI-1) in HepG2 cells. To this end, subconfluent HepG2 cells were incubated with triglyceride-rich particles (TGRP) isolated from In...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1997-05-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520372114 |
| _version_ | 1818675732724842496 |
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| author | C Banfi P Risé L Mussoni C Galli E Tremoli |
| author_facet | C Banfi P Risé L Mussoni C Galli E Tremoli |
| author_sort | C Banfi |
| collection | DOAJ |
| description | This study was undertaken in order to assess whether triglycerides and/or their fatty acids directly influence the secretion of plasminogen activator inhibitor type 1 (PAI-1) in HepG2 cells. To this end, subconfluent HepG2 cells were incubated with triglyceride-rich particles (TGRP) isolated from Intralipid for 16 h, and PAI-1 levels were determined in conditioned medium using a specific ELISA. TGRP (1 to 6 mg triglycerides/ml) concentration-dependently increased PAI-1 secretion by cells, concomitantly with significant increases in intracellular triglyceride (TG) levels. Fatty acid analysis indicated that the incubation of cells with 3 mg of TG per ml of TGRP induced significant accumulation of 18:2 n-6 (linoleic acid, LA) and 18:3 n-3 (linolenic acid) reflecting the fatty acid composition at the added triglycerides. We then tested the comparative effects on PAI-1 secretion by HepG2 cells of LA and 18:1 n-9 (oleic acid, OA). LA, as a bovine serum albumin (BSA) complex, concentration-dependently (1 to 35 mumol/L) increased the secretion of PAI-1 by cells, whereas OA-BSA only minimally affected it at the highest concentration used (35 mumol/L). Incorporation of LA into cell pools, in the presence of increasing concentration of the FA in the medium, was studied by the use of a preparation containing [14C]LA. LA accumulated in all lipid classes including diacylglycerol, the incorporated LA being converted into arachidonic acid (AA) as assessed by HPLC radiochromatography of the fatty acid methyl esters. It is concluded that PAI-1 secretion in HepG2 cells is modulated by triacylglycerols and by linoleic acid and/or its metabolic products. |
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| format | Article |
| id | doaj.art-7bf00822bb0448e1b4a5b6ebf1f2218a |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T08:32:16Z |
| publishDate | 1997-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-7bf00822bb0448e1b4a5b6ebf1f2218a2022-12-21T21:56:34ZengElsevierJournal of Lipid Research0022-22751997-05-01385860869Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cellsC Banfi0P Risé1L Mussoni2C Galli3E Tremoli4E. Grossi Paoletti Center, Institute of Pharmacological Sciences, University of Milan, Italy.E. Grossi Paoletti Center, Institute of Pharmacological Sciences, University of Milan, Italy.E. Grossi Paoletti Center, Institute of Pharmacological Sciences, University of Milan, Italy.E. Grossi Paoletti Center, Institute of Pharmacological Sciences, University of Milan, Italy.E. Grossi Paoletti Center, Institute of Pharmacological Sciences, University of Milan, Italy.This study was undertaken in order to assess whether triglycerides and/or their fatty acids directly influence the secretion of plasminogen activator inhibitor type 1 (PAI-1) in HepG2 cells. To this end, subconfluent HepG2 cells were incubated with triglyceride-rich particles (TGRP) isolated from Intralipid for 16 h, and PAI-1 levels were determined in conditioned medium using a specific ELISA. TGRP (1 to 6 mg triglycerides/ml) concentration-dependently increased PAI-1 secretion by cells, concomitantly with significant increases in intracellular triglyceride (TG) levels. Fatty acid analysis indicated that the incubation of cells with 3 mg of TG per ml of TGRP induced significant accumulation of 18:2 n-6 (linoleic acid, LA) and 18:3 n-3 (linolenic acid) reflecting the fatty acid composition at the added triglycerides. We then tested the comparative effects on PAI-1 secretion by HepG2 cells of LA and 18:1 n-9 (oleic acid, OA). LA, as a bovine serum albumin (BSA) complex, concentration-dependently (1 to 35 mumol/L) increased the secretion of PAI-1 by cells, whereas OA-BSA only minimally affected it at the highest concentration used (35 mumol/L). Incorporation of LA into cell pools, in the presence of increasing concentration of the FA in the medium, was studied by the use of a preparation containing [14C]LA. LA accumulated in all lipid classes including diacylglycerol, the incorporated LA being converted into arachidonic acid (AA) as assessed by HPLC radiochromatography of the fatty acid methyl esters. It is concluded that PAI-1 secretion in HepG2 cells is modulated by triacylglycerols and by linoleic acid and/or its metabolic products.http://www.sciencedirect.com/science/article/pii/S0022227520372114 |
| spellingShingle | C Banfi P Risé L Mussoni C Galli E Tremoli Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cells Journal of Lipid Research |
| title | Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cells |
| title_full | Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cells |
| title_fullStr | Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cells |
| title_full_unstemmed | Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cells |
| title_short | Linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by HepG2 cells |
| title_sort | linoleic acid enhances the secretion of plasminogen activator inhibitor type 1 by hepg2 cells |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520372114 |
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