Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins.
In recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western bl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0291743 |
| _version_ | 1797671885940457472 |
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| author | Michael Essien Sakyi Takashi Kamio Kaoru Kohyama Md Matiur Rahman Kaori Shimizu Ayaka Okada Yasuo Inoshima |
| author_facet | Michael Essien Sakyi Takashi Kamio Kaoru Kohyama Md Matiur Rahman Kaori Shimizu Ayaka Okada Yasuo Inoshima |
| author_sort | Michael Essien Sakyi |
| collection | DOAJ |
| description | In recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western blotting, have been used to detect antibodies against pathogens in marine mammals. However, options for commercial secondary antibodies used to detect antibodies in marine mammals are limited; therefore, the use of proteins A, G, or chimeric protein AG may provide a suitable alternative. This study aimed to assess the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. Currently, there are no comparative studies on the use of proteins A, G, and chimeric protein AG for the detection of immunoglobulins in marine mammals. In this study, we used ten pinnipeds' species (Baikal seal, California sea lion, harbor seal, northern fur seal, ringed seal, South American fur seal, South American sea lion, spotted seal, Steller sea lion, and walrus) and five cetacean species (beluga whale, bottlenose dolphin, harbor porpoise, killer whale, and Pacific white-sided dolphin) and compare binding ability to proteins A, G, or chimeric protein AG by ELISA. The results revealed that the immunoglobulins from pinniped and cetacean species reacted more strongly to protein A than protein G. In addition, the immunoglobulins of pinnipeds and cetaceans showed a strong binding ability to chimeric protein AG. These results suggest that proteins A, G, and chimeric protein AG would be used to help further develop serological assays. |
| first_indexed | 2024-03-11T21:22:00Z |
| format | Article |
| id | doaj.art-7bf2826bedb54d69a6e0d2908cc28800 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T21:22:00Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7bf2826bedb54d69a6e0d2908cc288002023-09-28T05:31:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01189e029174310.1371/journal.pone.0291743Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins.Michael Essien SakyiTakashi KamioKaoru KohyamaMd Matiur RahmanKaori ShimizuAyaka OkadaYasuo InoshimaIn recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western blotting, have been used to detect antibodies against pathogens in marine mammals. However, options for commercial secondary antibodies used to detect antibodies in marine mammals are limited; therefore, the use of proteins A, G, or chimeric protein AG may provide a suitable alternative. This study aimed to assess the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. Currently, there are no comparative studies on the use of proteins A, G, and chimeric protein AG for the detection of immunoglobulins in marine mammals. In this study, we used ten pinnipeds' species (Baikal seal, California sea lion, harbor seal, northern fur seal, ringed seal, South American fur seal, South American sea lion, spotted seal, Steller sea lion, and walrus) and five cetacean species (beluga whale, bottlenose dolphin, harbor porpoise, killer whale, and Pacific white-sided dolphin) and compare binding ability to proteins A, G, or chimeric protein AG by ELISA. The results revealed that the immunoglobulins from pinniped and cetacean species reacted more strongly to protein A than protein G. In addition, the immunoglobulins of pinnipeds and cetaceans showed a strong binding ability to chimeric protein AG. These results suggest that proteins A, G, and chimeric protein AG would be used to help further develop serological assays.https://doi.org/10.1371/journal.pone.0291743 |
| spellingShingle | Michael Essien Sakyi Takashi Kamio Kaoru Kohyama Md Matiur Rahman Kaori Shimizu Ayaka Okada Yasuo Inoshima Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. PLoS ONE |
| title | Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
| title_full | Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
| title_fullStr | Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
| title_full_unstemmed | Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
| title_short | Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
| title_sort | assessing of the use of proteins a g and chimeric protein ag to detect marine mammal immunoglobulins |
| url | https://doi.org/10.1371/journal.pone.0291743 |
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