Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene Expression
Luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) belong to the gonadotropic hormone receptors (GtHR), which are highly expressed in fish gonads and participate in the regulation of fish reproductive activities. Fish gonadal development and gamete maturation are not...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Fishes |
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| Online Access: | https://www.mdpi.com/2410-3888/7/6/316 |
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| author | Jie Yu Dayu Li Jinglin Zhu Zhiying Zou Wei Xiao Binglin Chen Hong Yang |
| author_facet | Jie Yu Dayu Li Jinglin Zhu Zhiying Zou Wei Xiao Binglin Chen Hong Yang |
| author_sort | Jie Yu |
| collection | DOAJ |
| description | Luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) belong to the gonadotropic hormone receptors (GtHR), which are highly expressed in fish gonads and participate in the regulation of fish reproductive activities. Fish gonadal development and gamete maturation are not only regulated by their BPG axis but also affected by natural environmental factors (such as temperature, salinity, pH, nutrients, light, etc.). <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>) is a farmed fish with a short reproductive cycle, fast growth, and high economic value. To study the relationship between gonadotropic hormone receptors (GtHR) and the reproductive activity of <i>Nile tilapia</i>, different oxytocin injection experiments and different temperature treatment experiments were set up, and the expression changes of the <i>GtHR</i> gene in the gonads and the concentration changes of the estradiol (E<sub>2</sub>) in the female serum and testosterone (T) in the male serum were determined employing a quantitative RT-PCR assay and enzyme-linked immunosorbent assay (ELISA), respectively. After the injection of oxytocin, with the change of E2 in females and T in males, the <i>FSHR</i> showed an expression pattern of first increase, then decrease, and the <i>LHR</i> showed an expression pattern of first increase, then decrease, and finally increase in the gonads, and the expression level of <i>FSHR</i> and <i>LHR</i> in the injection group was significantly higher than that in the control group at multiple time points; in addition, the expression level of <i>FSHR</i> and <i>LHR</i> in the oxytocin-combination injection group was higher than that in the single injection group. During 28 days of treatment at different temperatures, the sex steroid hormones and <i>GtHR</i> genes also showed regular changes, and the relationship between each group was 28 °C > 32 °C > 24 °C at most time points. According to the research results, it is speculated that <i>FSHR</i> and <i>LHR</i> play an important role in the development of <i>Nile tilapia</i> gonads and participate in the reproductive activities of <i>Nile tilapia</i>. By comparing and analyzing the changes in the sex steroid hormones and <i>GtHR</i> genes in each experimental group, it is speculated that different oxytocin injections could affect the expression of <i>FSHR</i> and <i>LHR</i> genes in <i>Nile tilapia</i>, and the combined effect of oxytocin was better than single oxytocin; the optimum temperature for the reproduction of <i>Nile tilapia</i> is between 28–32 °C. This study provides a theoretical basis for further elucidating the physiological functions and molecular mechanisms of FSHR and LHR and also provides a reference for the research of reproductive regulation in <i>Nile tilapia</i>. |
| first_indexed | 2024-03-09T16:45:04Z |
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| spelling | doaj.art-7bf2a119074843048b99f59a570369d62023-11-24T14:47:35ZengMDPI AGFishes2410-38882022-10-017631610.3390/fishes7060316Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene ExpressionJie Yu0Dayu Li1Jinglin Zhu2Zhiying Zou3Wei Xiao4Binglin Chen5Hong Yang6Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaLuteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) belong to the gonadotropic hormone receptors (GtHR), which are highly expressed in fish gonads and participate in the regulation of fish reproductive activities. Fish gonadal development and gamete maturation are not only regulated by their BPG axis but also affected by natural environmental factors (such as temperature, salinity, pH, nutrients, light, etc.). <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>) is a farmed fish with a short reproductive cycle, fast growth, and high economic value. To study the relationship between gonadotropic hormone receptors (GtHR) and the reproductive activity of <i>Nile tilapia</i>, different oxytocin injection experiments and different temperature treatment experiments were set up, and the expression changes of the <i>GtHR</i> gene in the gonads and the concentration changes of the estradiol (E<sub>2</sub>) in the female serum and testosterone (T) in the male serum were determined employing a quantitative RT-PCR assay and enzyme-linked immunosorbent assay (ELISA), respectively. After the injection of oxytocin, with the change of E2 in females and T in males, the <i>FSHR</i> showed an expression pattern of first increase, then decrease, and the <i>LHR</i> showed an expression pattern of first increase, then decrease, and finally increase in the gonads, and the expression level of <i>FSHR</i> and <i>LHR</i> in the injection group was significantly higher than that in the control group at multiple time points; in addition, the expression level of <i>FSHR</i> and <i>LHR</i> in the oxytocin-combination injection group was higher than that in the single injection group. During 28 days of treatment at different temperatures, the sex steroid hormones and <i>GtHR</i> genes also showed regular changes, and the relationship between each group was 28 °C > 32 °C > 24 °C at most time points. According to the research results, it is speculated that <i>FSHR</i> and <i>LHR</i> play an important role in the development of <i>Nile tilapia</i> gonads and participate in the reproductive activities of <i>Nile tilapia</i>. By comparing and analyzing the changes in the sex steroid hormones and <i>GtHR</i> genes in each experimental group, it is speculated that different oxytocin injections could affect the expression of <i>FSHR</i> and <i>LHR</i> genes in <i>Nile tilapia</i>, and the combined effect of oxytocin was better than single oxytocin; the optimum temperature for the reproduction of <i>Nile tilapia</i> is between 28–32 °C. This study provides a theoretical basis for further elucidating the physiological functions and molecular mechanisms of FSHR and LHR and also provides a reference for the research of reproductive regulation in <i>Nile tilapia</i>.https://www.mdpi.com/2410-3888/7/6/316<i>Nile tilapia</i>luteinizing hormone receptorfollicle-stimulating hormone receptoroxytocintemperaturegene expression |
| spellingShingle | Jie Yu Dayu Li Jinglin Zhu Zhiying Zou Wei Xiao Binglin Chen Hong Yang Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene Expression Fishes <i>Nile tilapia</i> luteinizing hormone receptor follicle-stimulating hormone receptor oxytocin temperature gene expression |
| title | Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene Expression |
| title_full | Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene Expression |
| title_fullStr | Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene Expression |
| title_full_unstemmed | Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene Expression |
| title_short | Effects of Different Oxytocin and Temperature on Reproductive Activity in <i>Nile tilapia</i> (<i>Oreochromis niloticus</i>): Based on Sex Steroid Hormone and GtHR Gene Expression |
| title_sort | effects of different oxytocin and temperature on reproductive activity in i nile tilapia i i oreochromis niloticus i based on sex steroid hormone and gthr gene expression |
| topic | <i>Nile tilapia</i> luteinizing hormone receptor follicle-stimulating hormone receptor oxytocin temperature gene expression |
| url | https://www.mdpi.com/2410-3888/7/6/316 |
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