Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method
The production of bioethanol as a clean liquid fuel in a cost-effective way is highly desired by global energetics. Sugar beet molasses is a renewable and cheap substrate for the production of biotechnological products. Therefore, the aim of the current study was the optimization of an industrial me...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-01-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/5/1/14 |
| _version_ | 1818924817679646720 |
|---|---|
| author | Farshad Darvishi Nooshin Abolhasan Moghaddami |
| author_facet | Farshad Darvishi Nooshin Abolhasan Moghaddami |
| author_sort | Farshad Darvishi |
| collection | DOAJ |
| description | The production of bioethanol as a clean liquid fuel in a cost-effective way is highly desired by global energetics. Sugar beet molasses is a renewable and cheap substrate for the production of biotechnological products. Therefore, the aim of the current study was the optimization of an industrial medium from molasses for bioethanol production using the Taguchi statistical experimental-design method. First, the growth rate of yeast cells and the amount of ethanol produced by the <i>Saccharomyces cerevisiae</i> strain sahand 101 were investigated in aerobic and aerobic⁻anaerobic conditions. The yeast strain produced 8% (<i>v</i>/<i>v</i>) bioethanol in a medium containing molasses with 18% Brix in aerobic⁻anaerobic conditions. The main factors of the medium, including molasses, ammonium sulfate, urea, and pH, were optimized for the increase of bioethanol production by the Taguchi method. Bioethanol production reached 10% (<i>v</i>/<i>v</i>) after optimization of the medium in flask culture. The yeast strain produced 11% (<i>v</i>/<i>v</i>) bioethanol in the bioreactor culture containing the optimized medium, which is an acceptable amount of bioethanol produced from molasses at the industrial scale. The results showed that the Taguchi method is an effective method for the design of experiments aiming to optimize the medium for bioethanol production by reducing the number of experiments and time. |
| first_indexed | 2024-12-20T02:31:22Z |
| format | Article |
| id | doaj.art-7bf3c0cc5a6d4de3af6b8539ecfd1d60 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-12-20T02:31:22Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-7bf3c0cc5a6d4de3af6b8539ecfd1d602022-12-21T19:56:34ZengMDPI AGFermentation2311-56372019-01-01511410.3390/fermentation5010014fermentation5010014Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design MethodFarshad Darvishi0Nooshin Abolhasan Moghaddami1Microbial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh 55181-83111, IranMicrobial Biotechnology and Bioprocess Engineering (MBBE) Group, Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh 55181-83111, IranThe production of bioethanol as a clean liquid fuel in a cost-effective way is highly desired by global energetics. Sugar beet molasses is a renewable and cheap substrate for the production of biotechnological products. Therefore, the aim of the current study was the optimization of an industrial medium from molasses for bioethanol production using the Taguchi statistical experimental-design method. First, the growth rate of yeast cells and the amount of ethanol produced by the <i>Saccharomyces cerevisiae</i> strain sahand 101 were investigated in aerobic and aerobic⁻anaerobic conditions. The yeast strain produced 8% (<i>v</i>/<i>v</i>) bioethanol in a medium containing molasses with 18% Brix in aerobic⁻anaerobic conditions. The main factors of the medium, including molasses, ammonium sulfate, urea, and pH, were optimized for the increase of bioethanol production by the Taguchi method. Bioethanol production reached 10% (<i>v</i>/<i>v</i>) after optimization of the medium in flask culture. The yeast strain produced 11% (<i>v</i>/<i>v</i>) bioethanol in the bioreactor culture containing the optimized medium, which is an acceptable amount of bioethanol produced from molasses at the industrial scale. The results showed that the Taguchi method is an effective method for the design of experiments aiming to optimize the medium for bioethanol production by reducing the number of experiments and time.https://www.mdpi.com/2311-5637/5/1/14bioethanolyeast<i>Saccharomyces cerevisiae</i>optimizationTaguchi method |
| spellingShingle | Farshad Darvishi Nooshin Abolhasan Moghaddami Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method Fermentation bioethanol yeast <i>Saccharomyces cerevisiae</i> optimization Taguchi method |
| title | Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method |
| title_full | Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method |
| title_fullStr | Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method |
| title_full_unstemmed | Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method |
| title_short | Optimization of an Industrial Medium from Molasses for Bioethanol Production Using the Taguchi Statistical Experimental-Design Method |
| title_sort | optimization of an industrial medium from molasses for bioethanol production using the taguchi statistical experimental design method |
| topic | bioethanol yeast <i>Saccharomyces cerevisiae</i> optimization Taguchi method |
| url | https://www.mdpi.com/2311-5637/5/1/14 |
| work_keys_str_mv | AT farshaddarvishi optimizationofanindustrialmediumfrommolassesforbioethanolproductionusingthetaguchistatisticalexperimentaldesignmethod AT nooshinabolhasanmoghaddami optimizationofanindustrialmediumfrommolassesforbioethanolproductionusingthetaguchistatisticalexperimentaldesignmethod |