Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress
Wheat cultivation makes an important contribution to human nutrition. Trehalose synthesis plays a role in the tolerance to drought stress. A bifunctional TPS-TPP enzyme gene from yeast was used to obtain transgenic wheat plants to increase trehalose synthesis. Mature wheat embryos were transformed u...
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MDPI AG
2023-06-01
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| Online Access: | https://www.mdpi.com/2076-3417/13/12/7267 |
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| author | Andrea Romero-Reyes Juan Pablo Valenzuela-Avendaño Ciria G. Figueroa-Soto José O. Mascorro-Gallardo Gabriel Iturriaga Alejandro Castellanos-Villegas Marisela Rivera-Domínguez Elisa M. Valenzuela-Soto |
| author_facet | Andrea Romero-Reyes Juan Pablo Valenzuela-Avendaño Ciria G. Figueroa-Soto José O. Mascorro-Gallardo Gabriel Iturriaga Alejandro Castellanos-Villegas Marisela Rivera-Domínguez Elisa M. Valenzuela-Soto |
| author_sort | Andrea Romero-Reyes |
| collection | DOAJ |
| description | Wheat cultivation makes an important contribution to human nutrition. Trehalose synthesis plays a role in the tolerance to drought stress. A bifunctional TPS-TPP enzyme gene from yeast was used to obtain transgenic wheat plants to increase trehalose synthesis. Mature wheat embryos were transformed using pGreen rd29A::TPS1-TPS2 or pGreen 35S::TPS1-TPS2 constructs. The transgene presence in mature leaves of T3 plants was confirmed by sequencing a PCR fragment of the inserted transgene. Transgenic and NT plants were submitted to drought stress for eight days. Transformed wheat lines retained a higher relative water content than NT plants during drought stress, and the Rubisco activity was unaffected. Plants transformed with the 35S construct showed a lower photosynthetic rate and lower fructose 1–6-bisphosphatase (FBPase) activity during drought, suggesting that constitutive trehalose and sucrose synthesis caused a reduced ribulose 1,5-bisphosphate (RuBP) regeneration. Lines transformed with the rd29A promoter showed a higher photosynthetic rate after eight days of drought, as the RuBP regeneration was unaffected. Transgenic wheat plants had higher biomass and grain weight than NT plants after drought. These results suggest that trehalose synthesis improves photosynthesis during stress and induces changes in the activity of some Calvin-cycle enzymes, reflected in plant metabolism and growth. |
| first_indexed | 2024-03-11T02:47:47Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T02:47:47Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-7bf50067b3de44a0adfe71fa0364b2d62023-11-18T09:11:29ZengMDPI AGApplied Sciences2076-34172023-06-011312726710.3390/app13127267Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought StressAndrea Romero-Reyes0Juan Pablo Valenzuela-Avendaño1Ciria G. Figueroa-Soto2José O. Mascorro-Gallardo3Gabriel Iturriaga4Alejandro Castellanos-Villegas5Marisela Rivera-Domínguez6Elisa M. Valenzuela-Soto7Centro de Investigación en Alimentación y Desarrollo A.C., Hermosillo 83304, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo A.C., Hermosillo 83304, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo A.C., Hermosillo 83304, Sonora, MexicoDepartamento de Fitotecnia, Universidad Autónoma Chapingo, Km 38.5 Carretera México-Texcoco, Texcoco 56230, Mexico, MexicoTecnológico Nacional de México/Campus Roque, Km 8 Carretera Celaya-Juventino Rosas, Roque Celaya, Guanajuato 38110, Guanajuato, MexicoDepartamento de Investigaciones Científicas y Tecnológicas, Universidad de Sonora, Reforma y L.D. Colosio, Hermosillo 83000, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo A.C., Hermosillo 83304, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo A.C., Hermosillo 83304, Sonora, MexicoWheat cultivation makes an important contribution to human nutrition. Trehalose synthesis plays a role in the tolerance to drought stress. A bifunctional TPS-TPP enzyme gene from yeast was used to obtain transgenic wheat plants to increase trehalose synthesis. Mature wheat embryos were transformed using pGreen rd29A::TPS1-TPS2 or pGreen 35S::TPS1-TPS2 constructs. The transgene presence in mature leaves of T3 plants was confirmed by sequencing a PCR fragment of the inserted transgene. Transgenic and NT plants were submitted to drought stress for eight days. Transformed wheat lines retained a higher relative water content than NT plants during drought stress, and the Rubisco activity was unaffected. Plants transformed with the 35S construct showed a lower photosynthetic rate and lower fructose 1–6-bisphosphatase (FBPase) activity during drought, suggesting that constitutive trehalose and sucrose synthesis caused a reduced ribulose 1,5-bisphosphate (RuBP) regeneration. Lines transformed with the rd29A promoter showed a higher photosynthetic rate after eight days of drought, as the RuBP regeneration was unaffected. Transgenic wheat plants had higher biomass and grain weight than NT plants after drought. These results suggest that trehalose synthesis improves photosynthesis during stress and induces changes in the activity of some Calvin-cycle enzymes, reflected in plant metabolism and growth.https://www.mdpi.com/2076-3417/13/12/7267carbohydratedroughtphotosynthesistrehalosewheat transformation |
| spellingShingle | Andrea Romero-Reyes Juan Pablo Valenzuela-Avendaño Ciria G. Figueroa-Soto José O. Mascorro-Gallardo Gabriel Iturriaga Alejandro Castellanos-Villegas Marisela Rivera-Domínguez Elisa M. Valenzuela-Soto Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress Applied Sciences carbohydrate drought photosynthesis trehalose wheat transformation |
| title | Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress |
| title_full | Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress |
| title_fullStr | Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress |
| title_full_unstemmed | Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress |
| title_short | Wheat Transformation with <i>ScTPS1-TPS2</i> Bifunctional Enzyme for Trehalose Biosynthesis Protects Photosynthesis during Drought Stress |
| title_sort | wheat transformation with i sctps1 tps2 i bifunctional enzyme for trehalose biosynthesis protects photosynthesis during drought stress |
| topic | carbohydrate drought photosynthesis trehalose wheat transformation |
| url | https://www.mdpi.com/2076-3417/13/12/7267 |
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