Structure of native chromatin fibres revealed by Cryo-ET in situ
Abstract The structure of chromatin plays pivotal roles in regulating gene transcription, DNA replication and repair, and chromosome segregation. This structure, however, remains elusive. Here, using cryo-FIB and cryo-ET, we delineate the 3D architecture of native chromatin fibres in intact interpha...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-42072-1 |
| _version_ | 1797557876364935168 |
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| author | Zhen Hou Frank Nightingale Yanan Zhu Craig MacGregor-Chatwin Peijun Zhang |
| author_facet | Zhen Hou Frank Nightingale Yanan Zhu Craig MacGregor-Chatwin Peijun Zhang |
| author_sort | Zhen Hou |
| collection | DOAJ |
| description | Abstract The structure of chromatin plays pivotal roles in regulating gene transcription, DNA replication and repair, and chromosome segregation. This structure, however, remains elusive. Here, using cryo-FIB and cryo-ET, we delineate the 3D architecture of native chromatin fibres in intact interphase human T-lymphoblasts and determine the in situ structures of nucleosomes in different conformations. These chromatin fibres are not structured as uniform 30 nm one-start or two-start filaments but are composed of relaxed, variable zigzag organizations of nucleosomes connected by straight linker DNA. Nucleosomes with little H1 and linker DNA density are distributed randomly without any spatial preference. This work will inspire future high-resolution investigations on native chromatin structures in situ at both a single-nucleosome level and a population level under many different cellular conditions in health and disease. |
| first_indexed | 2024-03-10T17:22:31Z |
| format | Article |
| id | doaj.art-7bf6990435a04087b22089f9ed313e33 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:22:31Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-7bf6990435a04087b22089f9ed313e332023-11-20T10:17:17ZengNature PortfolioNature Communications2041-17232023-10-011411710.1038/s41467-023-42072-1Structure of native chromatin fibres revealed by Cryo-ET in situZhen Hou0Frank Nightingale1Yanan Zhu2Craig MacGregor-Chatwin3Peijun Zhang4Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of OxfordDivision of Structural Biology, Wellcome Trust Centre for Human Genetics, University of OxfordDivision of Structural Biology, Wellcome Trust Centre for Human Genetics, University of OxfordDiamond Light Source, Harwell Science and Innovation CampusDivision of Structural Biology, Wellcome Trust Centre for Human Genetics, University of OxfordAbstract The structure of chromatin plays pivotal roles in regulating gene transcription, DNA replication and repair, and chromosome segregation. This structure, however, remains elusive. Here, using cryo-FIB and cryo-ET, we delineate the 3D architecture of native chromatin fibres in intact interphase human T-lymphoblasts and determine the in situ structures of nucleosomes in different conformations. These chromatin fibres are not structured as uniform 30 nm one-start or two-start filaments but are composed of relaxed, variable zigzag organizations of nucleosomes connected by straight linker DNA. Nucleosomes with little H1 and linker DNA density are distributed randomly without any spatial preference. This work will inspire future high-resolution investigations on native chromatin structures in situ at both a single-nucleosome level and a population level under many different cellular conditions in health and disease.https://doi.org/10.1038/s41467-023-42072-1 |
| spellingShingle | Zhen Hou Frank Nightingale Yanan Zhu Craig MacGregor-Chatwin Peijun Zhang Structure of native chromatin fibres revealed by Cryo-ET in situ Nature Communications |
| title | Structure of native chromatin fibres revealed by Cryo-ET in situ |
| title_full | Structure of native chromatin fibres revealed by Cryo-ET in situ |
| title_fullStr | Structure of native chromatin fibres revealed by Cryo-ET in situ |
| title_full_unstemmed | Structure of native chromatin fibres revealed by Cryo-ET in situ |
| title_short | Structure of native chromatin fibres revealed by Cryo-ET in situ |
| title_sort | structure of native chromatin fibres revealed by cryo et in situ |
| url | https://doi.org/10.1038/s41467-023-42072-1 |
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