Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut Seeds
Resveratrol (trans-3,4′,5-trihydroxystilbene) is a natural stilbene phytoalexin which is also found to be good for human health. Cultivated peanut (Arachis hypogaea L.), a worldwide important legume crop, is one of the few sources of human's dietary intake of resveratrol. Although the variation...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.644402/full |
| _version_ | 1818623417449971712 |
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| author | Huaiyong Luo Jianbin Guo Bolun Yu Weigang Chen Huan Zhang Xiaojing Zhou Yuning Chen Li Huang Nian Liu Xiaoping Ren Liying Yan Dongxin Huai Yong Lei Boshou Liao Huifang Jiang |
| author_facet | Huaiyong Luo Jianbin Guo Bolun Yu Weigang Chen Huan Zhang Xiaojing Zhou Yuning Chen Li Huang Nian Liu Xiaoping Ren Liying Yan Dongxin Huai Yong Lei Boshou Liao Huifang Jiang |
| author_sort | Huaiyong Luo |
| collection | DOAJ |
| description | Resveratrol (trans-3,4′,5-trihydroxystilbene) is a natural stilbene phytoalexin which is also found to be good for human health. Cultivated peanut (Arachis hypogaea L.), a worldwide important legume crop, is one of the few sources of human's dietary intake of resveratrol. Although the variations of resveratrol contents among peanut varieties were observed, the variations across environments and its underlying genetic basis were poorly investigated. In this study, the resveratrol content in seeds of a recombination inbred line (RIL) population (Zhonghua 6 × Xuhua 13, 186 progenies) were quantified by high performance liquid chromatography (HPLC) method across four environments. Genotypes, environments and genotype × environment interactions significantly influenced the resveratrol contents in the RIL population. A total of 8,114 high-quality single nucleotide polymorphisms (SNPs) were identified based on double-digest restriction-site-associated DNA sequencing (ddRADseq) reads. These SNPs were clustered into bins using a reference-based method, which facilitated the construction of high-density genetic map (2,183 loci with a total length of 2,063.55 cM) and the discovery of several chromosome translocations. Through composite interval mapping (CIM), nine additive quantitative trait loci (QTL) for resveratrol contents were identified on chromosomes A01, A07, A08, B04, B05, B06, B07, and B10 with 5.07–8.19% phenotypic variations explained (PVE). Putative genes within their confidential intervals might play roles in diverse primary and secondary metabolic processes. These results laid a foundation for the further genetic dissection of resveratrol content as well as the breeding and production of high-resveratrol peanuts. |
| first_indexed | 2024-12-16T18:40:44Z |
| format | Article |
| id | doaj.art-7bf7f3a9c1614d5da7324958d40fc533 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T18:40:44Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-7bf7f3a9c1614d5da7324958d40fc5332022-12-21T22:21:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-03-011210.3389/fpls.2021.644402644402Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut SeedsHuaiyong LuoJianbin GuoBolun YuWeigang ChenHuan ZhangXiaojing ZhouYuning ChenLi HuangNian LiuXiaoping RenLiying YanDongxin HuaiYong LeiBoshou LiaoHuifang JiangResveratrol (trans-3,4′,5-trihydroxystilbene) is a natural stilbene phytoalexin which is also found to be good for human health. Cultivated peanut (Arachis hypogaea L.), a worldwide important legume crop, is one of the few sources of human's dietary intake of resveratrol. Although the variations of resveratrol contents among peanut varieties were observed, the variations across environments and its underlying genetic basis were poorly investigated. In this study, the resveratrol content in seeds of a recombination inbred line (RIL) population (Zhonghua 6 × Xuhua 13, 186 progenies) were quantified by high performance liquid chromatography (HPLC) method across four environments. Genotypes, environments and genotype × environment interactions significantly influenced the resveratrol contents in the RIL population. A total of 8,114 high-quality single nucleotide polymorphisms (SNPs) were identified based on double-digest restriction-site-associated DNA sequencing (ddRADseq) reads. These SNPs were clustered into bins using a reference-based method, which facilitated the construction of high-density genetic map (2,183 loci with a total length of 2,063.55 cM) and the discovery of several chromosome translocations. Through composite interval mapping (CIM), nine additive quantitative trait loci (QTL) for resveratrol contents were identified on chromosomes A01, A07, A08, B04, B05, B06, B07, and B10 with 5.07–8.19% phenotypic variations explained (PVE). Putative genes within their confidential intervals might play roles in diverse primary and secondary metabolic processes. These results laid a foundation for the further genetic dissection of resveratrol content as well as the breeding and production of high-resveratrol peanuts.https://www.frontiersin.org/articles/10.3389/fpls.2021.644402/fullpeanutresveratrolhigh-density genetic mapQTLssingle nucleotide polymorphisms |
| spellingShingle | Huaiyong Luo Jianbin Guo Bolun Yu Weigang Chen Huan Zhang Xiaojing Zhou Yuning Chen Li Huang Nian Liu Xiaoping Ren Liying Yan Dongxin Huai Yong Lei Boshou Liao Huifang Jiang Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut Seeds Frontiers in Plant Science peanut resveratrol high-density genetic map QTLs single nucleotide polymorphisms |
| title | Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut Seeds |
| title_full | Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut Seeds |
| title_fullStr | Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut Seeds |
| title_full_unstemmed | Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut Seeds |
| title_short | Construction of ddRADseq-Based High-Density Genetic Map and Identification of Quantitative Trait Loci for Trans-resveratrol Content in Peanut Seeds |
| title_sort | construction of ddradseq based high density genetic map and identification of quantitative trait loci for trans resveratrol content in peanut seeds |
| topic | peanut resveratrol high-density genetic map QTLs single nucleotide polymorphisms |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.644402/full |
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