Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers
Epithelial Ovarian Cancer (EOC) is a leading cause of cancer-related deaths among women, mainly due to a lack of early detection and screening methods. Advanced immunoassay techniques, such as Luminex and proximity extension assay (PEA) technology, show promise in improving EOC detection by utilizin...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/23/5607 |
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| author | Vikneswary Ravi Kumar Nirmala Chandralega Kampan Nor Haslinda Abd Aziz Chew Kah Teik Mohamad Nasir Shafiee P. Susthitha Menon |
| author_facet | Vikneswary Ravi Kumar Nirmala Chandralega Kampan Nor Haslinda Abd Aziz Chew Kah Teik Mohamad Nasir Shafiee P. Susthitha Menon |
| author_sort | Vikneswary Ravi Kumar |
| collection | DOAJ |
| description | Epithelial Ovarian Cancer (EOC) is a leading cause of cancer-related deaths among women, mainly due to a lack of early detection and screening methods. Advanced immunoassay techniques, such as Luminex and proximity extension assay (PEA) technology, show promise in improving EOC detection by utilizing highly sensitive and specific multiplex panels to detect multiple combinations of biomarkers. However, these advanced immunoassay techniques have certain limitations, especially in validating the performance characteristics such as specificity, sensitivity, limit of detection (LOD), and dynamic range for each EOC biomarker within the panel. Implementing multiplexing in point-of-care (POC) biosensors can enhance EOC biomarker detection, with Surface Plasmon Resonance (SPR) being a versatile option among optical biosensors. There is no study on multiplex SPR biosensors specifically tailored for diagnosing EOC. Recent studies have shown promising results in the single detection of EOC biomarkers using SPR, with LOD for cancer antigen 125 (CA125) at 0.01 U/mL<sup>−1</sup> and human epididymis protein 4 (HE4) at 1pM. This study proposes a potential roadmap for scientists and engineers in academia and industry to develop a cost effective yet highly efficient SPR biosensor platform for detecting EOC. |
| first_indexed | 2024-03-09T01:53:54Z |
| format | Article |
| id | doaj.art-7bf95d6955924a20847f020e0548b07f |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T01:53:54Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-7bf95d6955924a20847f020e0548b07f2023-12-08T15:12:41ZengMDPI AGCancers2072-66942023-11-011523560710.3390/cancers15235607Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer BiomarkersVikneswary Ravi Kumar0Nirmala Chandralega Kampan1Nor Haslinda Abd Aziz2Chew Kah Teik3Mohamad Nasir Shafiee4P. Susthitha Menon5Department of Obstetrics and Gynaecology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaDepartment of Obstetrics and Gynaecology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaDepartment of Obstetrics and Gynaecology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaDepartment of Obstetrics and Gynaecology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaDepartment of Obstetrics and Gynaecology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur 56000, MalaysiaInstitute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia (UKM), Bangi 43600, MalaysiaEpithelial Ovarian Cancer (EOC) is a leading cause of cancer-related deaths among women, mainly due to a lack of early detection and screening methods. Advanced immunoassay techniques, such as Luminex and proximity extension assay (PEA) technology, show promise in improving EOC detection by utilizing highly sensitive and specific multiplex panels to detect multiple combinations of biomarkers. However, these advanced immunoassay techniques have certain limitations, especially in validating the performance characteristics such as specificity, sensitivity, limit of detection (LOD), and dynamic range for each EOC biomarker within the panel. Implementing multiplexing in point-of-care (POC) biosensors can enhance EOC biomarker detection, with Surface Plasmon Resonance (SPR) being a versatile option among optical biosensors. There is no study on multiplex SPR biosensors specifically tailored for diagnosing EOC. Recent studies have shown promising results in the single detection of EOC biomarkers using SPR, with LOD for cancer antigen 125 (CA125) at 0.01 U/mL<sup>−1</sup> and human epididymis protein 4 (HE4) at 1pM. This study proposes a potential roadmap for scientists and engineers in academia and industry to develop a cost effective yet highly efficient SPR biosensor platform for detecting EOC.https://www.mdpi.com/2072-6694/15/23/5607epithelial ovarian cancer (EOC)biomarkerscancer antigen 125 (CA125)human epididymis protein 4 (HE4)immunoassaymultiplex |
| spellingShingle | Vikneswary Ravi Kumar Nirmala Chandralega Kampan Nor Haslinda Abd Aziz Chew Kah Teik Mohamad Nasir Shafiee P. Susthitha Menon Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers Cancers epithelial ovarian cancer (EOC) biomarkers cancer antigen 125 (CA125) human epididymis protein 4 (HE4) immunoassay multiplex |
| title | Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers |
| title_full | Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers |
| title_fullStr | Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers |
| title_full_unstemmed | Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers |
| title_short | Recent Advances in Surface Plasmon Resonance (SPR) Technology for Detecting Ovarian Cancer Biomarkers |
| title_sort | recent advances in surface plasmon resonance spr technology for detecting ovarian cancer biomarkers |
| topic | epithelial ovarian cancer (EOC) biomarkers cancer antigen 125 (CA125) human epididymis protein 4 (HE4) immunoassay multiplex |
| url | https://www.mdpi.com/2072-6694/15/23/5607 |
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