Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies
Abstract This study introduces a novel diagnostic modality for the detection of feline panleukopenia virus (FPV) antibodies in feline serum by using fluorescent microsphere immunochromatographic test strips (FM-ICTS). Leveraging the inherent specificity of antigen-antibody interactions, the FM-ICTS...
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| Format: | Article |
| Language: | English |
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BMC
2024-09-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12896-024-00900-2 |
| _version_ | 1827098614352052224 |
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| author | Jinyuan Shang Manping Yan Xiaohao Zhang Wei Liu Shun Wu Zhenjun Wang Li Yi Chunxia Wang Erkai Feng Yuening Cheng Guoliang Luo |
| author_facet | Jinyuan Shang Manping Yan Xiaohao Zhang Wei Liu Shun Wu Zhenjun Wang Li Yi Chunxia Wang Erkai Feng Yuening Cheng Guoliang Luo |
| author_sort | Jinyuan Shang |
| collection | DOAJ |
| description | Abstract This study introduces a novel diagnostic modality for the detection of feline panleukopenia virus (FPV) antibodies in feline serum by using fluorescent microsphere immunochromatographic test strips (FM-ICTS). Leveraging the inherent specificity of antigen-antibody interactions, the FM-ICTS approach demonstrates considerable potential for efficient and accurate FPV antibody detection within a short timeframe. The FM-ICTS method demonstrates strong diagnostic performance, with consistent accuracy and stability over time. PBS buffer dilution enables detection across the range of FPV antibody haemagglutination inhibition (HI) titres in both healthy and immunized or infected cats. A high correlation (R² = 0.9733) between the T/C ratio and FPV antibody titres confirms the method’s effectiveness in quantifying these titres. Clinical validation with 84 samples supports its reliability by matching results with HI assays. Additionally, stability tests show that the test strips maintain performance during storage, with a coefficient of variation (CV) below 12% over three months at 25℃. This innovative FM-ICTS framework emerges as a promising avenue for expedient and dependable disease diagnosis within the realm of veterinary science, offering implications for timely disease management and surveillance. |
| first_indexed | 2025-03-20T07:50:22Z |
| format | Article |
| id | doaj.art-7bf9b2c932cc4a60b96f4b39c29d60f1 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2025-03-20T07:50:22Z |
| publishDate | 2024-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-7bf9b2c932cc4a60b96f4b39c29d60f12024-09-29T11:29:17ZengBMCBMC Biotechnology1472-67502024-09-012411810.1186/s12896-024-00900-2Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodiesJinyuan Shang0Manping Yan1Xiaohao Zhang2Wei Liu3Shun Wu4Zhenjun Wang5Li Yi6Chunxia Wang7Erkai Feng8Yuening Cheng9Guoliang Luo10Key Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesDepartment of Cardiology, The Second Hospital of Jilin UniversityXinuo BioTechnology Co., LtdKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesKey Laboratory of Economic Animal Diseases, Ministry of Agriculture, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural SciencesAbstract This study introduces a novel diagnostic modality for the detection of feline panleukopenia virus (FPV) antibodies in feline serum by using fluorescent microsphere immunochromatographic test strips (FM-ICTS). Leveraging the inherent specificity of antigen-antibody interactions, the FM-ICTS approach demonstrates considerable potential for efficient and accurate FPV antibody detection within a short timeframe. The FM-ICTS method demonstrates strong diagnostic performance, with consistent accuracy and stability over time. PBS buffer dilution enables detection across the range of FPV antibody haemagglutination inhibition (HI) titres in both healthy and immunized or infected cats. A high correlation (R² = 0.9733) between the T/C ratio and FPV antibody titres confirms the method’s effectiveness in quantifying these titres. Clinical validation with 84 samples supports its reliability by matching results with HI assays. Additionally, stability tests show that the test strips maintain performance during storage, with a coefficient of variation (CV) below 12% over three months at 25℃. This innovative FM-ICTS framework emerges as a promising avenue for expedient and dependable disease diagnosis within the realm of veterinary science, offering implications for timely disease management and surveillance.https://doi.org/10.1186/s12896-024-00900-2Fluorescence microsphereSerodiagnosisAntibody assayFeline panleukopenia virusImmunochromatography |
| spellingShingle | Jinyuan Shang Manping Yan Xiaohao Zhang Wei Liu Shun Wu Zhenjun Wang Li Yi Chunxia Wang Erkai Feng Yuening Cheng Guoliang Luo Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies BMC Biotechnology Fluorescence microsphere Serodiagnosis Antibody assay Feline panleukopenia virus Immunochromatography |
| title | Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies |
| title_full | Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies |
| title_fullStr | Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies |
| title_full_unstemmed | Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies |
| title_short | Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies |
| title_sort | preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies |
| topic | Fluorescence microsphere Serodiagnosis Antibody assay Feline panleukopenia virus Immunochromatography |
| url | https://doi.org/10.1186/s12896-024-00900-2 |
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